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General Information
Symbol
Dmel\Notum3
Species
D. melanogaster
Name
FlyBase ID
FBal0138446
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP-Notum
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C16030778T

Amino acid change:

Q485term | Notum-PA

Reported amino acid change:

Q485term

Comment:

The Notum3 mutation was induced on the NotumEPg31259 chromosome which carries a P{EPg} insertion approximately 1kb upstream of Notum. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{EPg}NotumEPg31259 insertion is also still present on the chromosome.

Amino acid replacement: Q485term.

Insertion components
P{EPg}NotumEPg31259
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Clones of Notum3 in pupal eyes show an increase in the number of peripheral ommatidia that undergo apoptosis and a corresponding expansion of the pigment rim.

Homozygous embryos derived from females containing homozygous germline clones have a range of severe defects. Most of these embryos have abnormal morphology; 43% have a posterior end that is not completely retracted, 12% are U-shaped and 34% are shaped like a ball. Of those embryos where the ventral cuticle can be reliably analysed, nearly all have thinner denticle belts in the abdomen than normal, and many have either patches of naked cuticle within the denticle belts, or missing denticle belts. Paternally rescued embryos derived from females containing homozygous germline clones hatch and do not show any morphological defects.

Notum3/NotumI6 larval wing discs show defects ranging from duplication of the wing pouch and hinge associated with a reduced thorax region to almost complete loss of the thorax region associated with a severely abnormal wing duplication. Large clones in the wing disc produce additional rows of sensory organ precursor cells, resulting in extra mechanosensory bristles in the anterior wing margin. These defects only occur when the clone affects both sides of the dorsoventral boundary. Some of the ectopic bristles are derived from wild-type cells neighbouring the clone.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

dlp2 +/+ Notum3 transheterozygotes show increased bristle density at the anterior wing margin and ectopic bristles positioned further from the wing margin than in wild type. Neither dlp2/+ or Notum3/+ single mutant flies show this phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)