Allele Dmel\Gp150³

General Information
Symbol	Dmel\Gp150³	Species	D. melanogaster
Name		FlyBase ID	FBal0138496
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Gp150

Allele class	amorph, loss of function
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	amorph (Fetchko et al., 2002) loss of function (Melnattur et al., 2002)
Mutagen	ethyl methanesulfonate (Fetchko et al., 2002)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Gp150^k11120b) (Fetchko et al., 2002) semi-lethal (Melnattur et al., 2002) semi-lethal (with Gp150^52A) (Melnattur et al., 2002) visible (Fetchko et al., 2002) female sterile (with Gp150^52A) (Melnattur et al., 2002)
Phenotype Manifest In
	eye (Fetchko et al., 2002) ommatidium (Fetchko et al., 2002) wing margin bristle (Fetchko et al., 2002) wing margin bristle \| ectopic (Fetchko et al., 2002) wing vein L3 \| ectopic (Fetchko et al., 2002) ommatidium \| somatic clone (Li et al., 2003) eye photoreceptor cell \| somatic clone (Li et al., 2003) anterior scutellar bristle (with Gp150^k11120b) (Fetchko et al., 2002) photoreceptor cell R8 (with Gp150^k11120b) (Fetchko et al., 2002) photoreceptor cell R1 (with Gp150^k11120b) (Fetchko et al., 2002) photoreceptor cell R6 (with Gp150^k11120b) (Fetchko et al., 2002) ommatidium (with Gp150^k11120b) (Fetchko et al., 2002) eye (with Gp150^k11120b) (Fetchko et al., 2002) eye (with Df(2R)02311) (Fetchko et al., 2002) ommatidium (with Df(2R)02311) (Fetchko et al., 2002) eye photoreceptor cell (with Gp150⁴) (Li et al., 2003) ommatidium (with Gp150⁴) (Li et al., 2003) eye (with Gp150⁴) (Li et al., 2003)
Detailed Description
	Statement Reference 45% of ommatidia are mutant in Gp150^k11120b/Gp150³ animals, 29% in Gp150³/Df(2R)02311. Over 60% of Gp150^k11120b/Gp150³ flies fail to develop to adulthood with most of them dying during pupal development. 2/3 of the anterior scutellars in surviving adults, exhibit no shaft and no socket, 1/4 have double sockets, 3% have one shaft and two sockets and another 3% have two shafts and one socket. 45% of ommatidia are mutant in Gp150^k11120b/Gp150³ animals. Developing ommatidial clusters are often irregularly spaced and the morphogenetic furrow appears broader as the cells within the region are no longer constricted in a narrow stripe. Multiple R8 precursor cells are often closely located, about 10% of ommatidia contain multiple R8 precursors that become R8 cells. Some oversized ommatidia are seen at early stages of ommatidia assembly. Some ommatidia contain additional or fewer R1/R6 cells. (Fetchko et al., 2002) Approximately 80% of Gp150²/Gp150^52A transheterozygotes survive to adulthood. Females are sterile and fail to lay eggs. (Melnattur et al., 2002) In Gp150³/Gp150⁴ animals the spacing of ommatidia is abnormal and some ommatidia have fewer or more photoreceptor cells. Mutants have 'rough' eyes. When homozygous clones are made in the eye mutant ommatidia are seen. No specific photoreceptor cell type is found to be mutant at a higher proportion than the others in these clones. (Li et al., 2003)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement Reference Gp150⁴/Gp150³ is a non-enhancer of eye phenotype of sca^BP2 (Li et al., 2003) Gp150⁴/Gp150³ is a non-enhancer of ommatidium phenotype of sca^BP2 (Li et al., 2003)
Suppressor of
Statement Reference Gp150⁴/Gp150³ is a suppressor of wing margin phenotype of Scer\GAL4^dpp.blk1, sca^Scer\UAS.cEa (Li et al., 2003)
NOT Suppressor of
Statement Reference Gp150⁴/Gp150³ is a non-suppressor of ommatidium phenotype of sca^BP2 (Li et al., 2003) Gp150⁴/Gp150³ is a non-suppressor of eye phenotype of sca^BP2 (Li et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The addition of Gp150³/Gp150⁴ has no effect on the sca^BP2 eye phenotype When sca^Scer\UAS.cEa is driven by Scer\GAL4^dpp.blk1 in a Gp150⁴/Gp150⁴ background, no wing margin loss is seen.. (Li et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	defu³ (Li et al., 2003) gp150³ (Melnattur et al., 2002) Gp150³ gp1503 (Li et al., 2004)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Li et al., 2003, Development 130(13): 2819--2827 Scabrous and Gp150 are endosomal proteins that regulate Notch activity. [FBrf0161465] Fetchko et al., 2002, EMBO J. 21(5): 1074--1083 Drosophila Gp150 is required for early ommatidial development through modulation of Notch signaling. [FBrf0144849] Melnattur et al., 2002, genesis 34(1-2): 29--33 P(52A-GAL4) is an insertion in the Drosophila gp150 gene. [FBrf0151713]
Erratum	Li et al., 2004, Development 131(20): 5213 Scabrous and Gp150 are endosomal proteins that regulate Notch activity. [FBrf0188446]

Allele Dmel\Gp1503

Allele Dmel\Gp150³