Allele Dmel\Fadd^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Fadd^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0138577
Feature type	allele	Associated gene	Dmel\Fadd

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Leulier et al., 2002) in vitro construct - RNAi (Leulier et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Nucleotides 1-500 of the BG4 cDNA has been inserted as an inverted repeat and driven by Scer\UAS sequences. (Leulier et al., 2002)
Carried in construct	P{UAS-BG4-dsRNA} (Kleino et al., 2005, Tang et al., 2006, Leulier et al., 2002, Kambris et al., 2006, Libert et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^c564 (Kleino et al., 2005) immune response defective, with Scer\GAL4^da.G32 (Tang et al., 2006) immune response defective, with Scer\GAL4^yolk (Leulier et al., 2002) wild-type, with Scer\GAL4^da.G32 (Leulier et al., 2002)
Phenotype Manifest In

Detailed Description
	Statement Reference Adult flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show a reduced survival rate compared to control flies after bacterial (E.carotovora) infection. (Tang et al., 2006) The immune response to infection with the Gram-negative bacteria P.carotovorum, as determined by induction of Dpt, is reduced when BG4^{dsRNA.Scer\UAS} expression is driven in the fat body by Scer\GAL4^c564. (Kleino et al., 2005) When BG4^{dsRNA.Scer\UAS} is driven by Scer\GAL4^yolk, mutant animals are highly susceptible to Gram-negative bacterial infection, exhibiting a dramatic loss of survival rate after infection, with almost all animals dying within 4 days. However, mutants appear resistant to fungal infection. Expression of BG4^{dsRNA.Scer\UAS} when driven by Scer\GAL4^da.G32 shows no detectable developmental defects. (Leulier et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Fadd^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 has immune response defective phenotype, enhanceable by MP1^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^da.G32 (Tang et al., 2006) Fadd^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 has immune response defective phenotype, enhanceable by Sp7^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^da.G32 (Tang et al., 2006)
Suppressor of
Statement Reference Fadd^{dsRNA.Scer\UAS}, Scer\GAL4^S106-GS is a suppressor \| partially of immune response defective phenotype of PGRP-LE^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^S106-GS (Libert et al., 2006)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Simultaneous expression of BG4[dsRNA.Scer\UAS] driven by Scer\GAL4[S106-GS] significantly suppresses the increased pathogen resistance phenotype of PGRP-LE[Scer\UAS.T:Zzzz\FLAG]-expressing flies. (Libert et al., 2006) Co-expression of either MP1^{dsRNA.Scer\UAS.WIZ} or Sp7^{dsRNA.Scer\UAS.WIZ} results in a moderate further decrease in the survival rate of flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 after bacterial (E.carotovora) infection. (Tang et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Transcription from the P{UAS-BG4-dsRNA} construct should produce BG4 dsRNA, resulting in dsRNA interference (RNAi) of the BG4 gene. (Leulier et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	BG4^{dsRNA.Scer\UAS} Fadd^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Kambris et al., 2006, Curr. Biol. 16(8): 808--813 Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. [FBrf0190023] Libert et al., 2006, Aging Cell 5(6): 533--543 Trade-offs between longevity and pathogen resistance in Drosophila melanogaster are mediated by NFkappaB signaling. [FBrf0192958] Tang et al., 2006, J. Biol. Chem. 281(38): 28097--28104 Two proteases defining a melanization cascade in the immune system of Drosophila. [FBrf0194554] Kleino et al., 2005, EMBO J. 24(19): 3423--3434 Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway. [FBrf0190362] Leulier et al., 2002, Curr. Biol. 12(12): 996--1000 Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults. [FBrf0151243]

Allele Dmel\FadddsRNA.Scer\UAS

Allele Dmel\Fadd^{dsRNA.Scer\UAS}