Allele Dmel\slam^waldo1

General Information
Symbol	Dmel\slam^waldo1	Species	D. melanogaster
Name		FlyBase ID	FBal0140930
Feature type	allele	Associated gene	Dmel\slam

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Stein et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:6,375,806..6,375,806 reported_pr_change=Q722@ comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Annotated protein is 23 amino acids longer than the reported protein accounting for the difference in reported and annotated mutation location. evidence=experimental na_change=C6375806T pr_change=Q745@\|slam-PA (Stein et al., 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q722@. (Stein et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	cycle 14 embryo (with slam^waldo2) (Stein et al., 2002) germline cell (Stein et al., 2002)
Detailed Description
	Statement Reference Mutant embryos have defects in germ cell migration; about 50% of the germ cells are lost by the end of embryogenesis. slam^waldo1/slam^waldo2 embryos derived from homozygous slam^waldo1 female germline clones (embryos lack both maternal and zygotic slam function) fail to cellularise during nuclear cycle 14. Germ cells form normally during cycle 10 in these embryos, and are later found distributed throughout an otherwise unstructured embryo. Nuclei reach the periphery normally in mutant precellularisation embryos, the primordial germ cells bud normally and the somatic nuclei continue to divide until cycle 14. The first difference from wild-type is seen during the slow phase of cellularisation; membrane invagination is delayed in the mutant embryos, so that at the time when membranes normally enclose each nucleus basally, the incompletely invaginated membranes of the mutant embryos entrap the nuclei as they pinch off basally. The mutant embryos do attempt to gastrulate, but this disrupts the incompletely formed somatic cells and the embryos appear to fall apart and fail to develop further. (Stein et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference slam^waldo1 is a non-suppressor of germline cell phenotype of Hmgcr^{Scer\UAS.cvDa}, Scer\GAL4^elav-C155 (Stein et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Expression of Hmgcr^{Scer\UAS.cvDa} under the control of Scer\GAL4^elav-C155 can attract germ cells in embryos. This phenotype is still seen in a slam^waldo1 mutant background. The germ cell migration defects of slam^waldo1 Hmgcr^clb1 double mutants are stronger than either single mutant; no germ cells move off the midgut in double mutant embryos. (Stein et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	slam^waldo1 waldo¹ (Stein et al., 2002)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Stein et al., 2002, Development 129(16): 3925--3934 Slow as Molasses is required for polarized membrane growth and germ cell migration in Drosophila. [FBrf0151273]

Allele Dmel\slamwaldo1

Allele Dmel\slam^waldo1