Open Close
General Information
Symbol
Dmel\perUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Blanchardon
FlyBase ID
FBal0140982
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-per, uas-per16
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 3.9 kb per cDNA (beginning 46 bp upstream from the ATG and ending at position 4230 in the mRNA, in the 3' untranslated region).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

per01 animals expressing perScer\UAS.cBa under the control of Scer\GAL4P2.4.Pdf or Scer\GAL4Mz520 have a normal morning activity peak during LD conditions but do not have a normal evening activity peak. In DD conditions, show an activity bout in the subjective day that is about 6 hours before the one seen in wild-type animals. per01 animals expressing perScer\UAS.cBa under the control of Scer\GAL4Mai179 display a robust anticipation of both lights-on and lights off transitions. In DD conditions, per01 animals expressing perScer\UAS.cBa under the control of either Scer\GAL4P2.4.Pdf or Scer\GAL4Mz520 and Scer\GAL4Mai179, exhibit an extended activity bout in the subjective day that starts about 6 hours before wild-type and continuing towards the end of the subjective day.

Scer\GAL41118-driven perScer\UAS.cBa expression abolishes activity and eclosion rhythms. Scer\GAL41118-driven perScer\UAS.cBa expression suppresses activity and eclosion rhythms in a perScer\UAS.cBa dose dependent manner, as the number of arrhythmic flies strongly increases between the heterozygous Scer\GAL41118 perScer\UAS.cBa/+ and the homozygous Scer\GAL41118 perScer\UAS.cBa genotypes. However, anticipation of the lights-off period transition can be be observed in light-dark conditions at a varying degree. No phase advance is observed for the anticipation of of lights-off. In the same line, no weak short-period rhythm could be detected in constant darkness.

No weak short-period weak rhythm can be detected in Scer\GAL41118-driven perScer\UAS.cBa expressing lines, even during the first days in constant darkness.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The aberrant long-period circadian locomotor rhythm under free-running constant dark conditions characteristic for Lsm12Δ6 mutant adults is partially rescued by Scer\GAL4cry.PE-driven expression of perScer\UAS.cBa.

The weakened rhythmicity seen in tyfΔG14151 and tyfe00614 flies under constant darkness conditions is suppressed by expression of perScer\UAS.cBa under the control of Scer\GAL4P2.4.Pdf.

The length of the circadian cycle is increased when perScer\UAS.cBa is expressed in homozygous cwoPB9 flies with one copy of per01 under the control of either a Scer\GAL4tim.PE or a Scer\GAL4cry.PE driver.

In contrast to wild-type animals, per01; ; cryb double mutants expressing Scer\GAL80Pdf.PS and Scer\GAL4Mai179 driven perScer\UAS.cBa display robust locomotor activity rhythms, with a long 25.7h period under constant light conditions.

Xenogenetic Interactions
Statement
Reference

per01 mutant files co-expressing perScer\UAS.cBa and Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4Mai179 display morning anticipation in their locomotor activity, but they completely lose evening anticipation, compared with controls with Ctet\TeTxLCIMPTNT-V.Scer\UAS expression.

Anticipatory activity is absent in the morning and almost completely lost in the evening in per01 mutants co-expressing perScer\UAS.cBa and Ctet\TeTxLCTNT.Scer\UAS under the control of both Scer\GAL4Mai179 and Scer\GAL80Pdf.PS.

Complementation and Rescue Data
Comments

Expression of perScer\UAS.cBa under the control of Scer\GAL4Clk.1.5 partially rescues the circadian locomotor activity defects seen in per01 flies under light-dark conditions; the rescued flies show a robust anticipation of the lights-on transition (morning peak), but evening anticipation is extremely weak and difficult to distinguish from an increase in locomotor activity also observed in control per01 flies at the end of the day.

Expression of perScer\UAS.cBa under the control of Scer\GAL4Clk.1.5 in per01 flies tested under a 12 hours 29[o]C/12 hours 20[o]C thermal cycle in constant darkness rescues the evening activity peak.

Expression of perScer\UAS.cBa under the control of Scer\GAL4tim.PE (per expression in all clock neurons) in per01 flies results in animals that show a similar locomotor activity to wild-type flies, except that the rescued flies have a later evening peak under 12:12 hour light-dark conditions and a longer period under constant darkness conditions.

per01 flies that are expressing perScer\UAS.cBa under the control of Scer\GAL4Mai179 (per expression in the majority of the lateral neurons) show bimodal locomotor activity patterns under 12:12 hour light-dark conditions. The flies show an advanced morning peak of activity. These flies show morning and evening peaks of locomotor activity that shift into night under 12:12 hour light moonlight conditions (under these conditions, a dim light of quarter-moonlight intensity, 0.03 lux, is switched on during the dark phase), although the morning peak is strongly reduced and is not present in all flies. When it is present, it occurs 1.4 hours earlier than in controls. Under constant moonlight conditions, the morning peak is further reduced and is only visible in 50% of the rescued flies. Most of the rescued flies remain rhythmic under constant moonlight or constant darkness conditions.

per01 flies that are expressing perScer\UAS.cBa under the control of Scer\GAL4Mai179 in the presence of Scer\GAL80Pdf.PS (this restricts expression of per to the 5th s-LNv and the 3 cry-positive LNd lateral neurons) have a prominent lights-on peak of locomotor activity. The morning peak occurs before lights-on and appears suppressed in most flies, and the average activity increase that occurs before lights-on is not different from non-rescued per01 flies (although it is possible to determine the phase of the morning peak in individual rescued flies). Under 12:12 hour light moonlight conditions (under these conditions, a dim light of quarter-moonlight intensity, 0.03 lux, is switched on during the dark phase) the rescued flies show morning and evening peaks of locomotor activity that are shifted into the night. Under constant moonlight conditions approximately 50% of the rescued flies remain rhythmic with a wide phase relationship between morning and evening peaks. Under constant darkness conditions, all of the rescued flies become arrhythmic.

In contrast to wild-type animals, per01 mutants expressing Scer\GAL4P2.4.Pdf driven perScer\UAS.cBa do display locomotor rhythms under constant darkness.

Similarly to wild-type animals, per01 mutants expressing Scer\GAL80Pdf.PS and Scer\GAL4Mai179 or Scer\GAL4cry.PE driven perScer\UAS.cBa do not display locomotor acivity rhythms under constant darkness.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
perScer\UAS.cBa
perUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Blanchardon
Secondary FlyBase IDs
    References (14)