Allele Dmel\nos^{αTub84B.3'UTR.nos+2.Scer\UAS}

General Information
Symbol	Dmel\nos^{αTub84B.3'UTR.nos+2.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0141014
Feature type	allele	Associated gene	Dmel\nos

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Clark et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	nos^{αTub84B.3'UTR.Scer\UAS} (Clark et al., 2002)
Nature of the lesion	Statement Reference Construct: A 180bp nucleotide region of the nos 3'UTR (the "+2 element") has been inserted into a BstEII site within the αTub84B 3'UTR sequences. (Clark et al., 2002)
Carried in construct	P{UAS-nos-tub:nos+2} (Duchow et al., 2005, Clark et al., 2002, Ye et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^h-1J3 (Clark et al., 2002) lethal, with Scer\GAL4^how-24B (Clark et al., 2002) lethal \| partially (Clark et al., 2002) lethal \| partially, with Scer\GAL4^elav.PLu (Clark et al., 2002) neuroanatomy defective, with Scer\GAL4^8-123 (Ye et al., 2004) partially lethal - majority die, with Scer\GAL4^HD34A (Duchow et al., 2005) visible, with Scer\GAL4^GMR.PF (Clark et al., 2002) visible, with Scer\GAL4^hs.2sev (Clark et al., 2002)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4^Eh.2.4 (Clark et al., 2002) adult cuticle, with Scer\GAL4^HD23A (Clark et al., 2002) adult cuticle, with Scer\GAL4^HD44A (Clark et al., 2002) adult cuticle, with Scer\GAL4^hs.2sev (Clark et al., 2002) adult cuticle, with Scer\GAL4^sca-109-68 (Clark et al., 2002) dendritic arborising neuron & dendritic tree, with Scer\GAL4^8-123 (Ye et al., 2004) ommatidium, with Scer\GAL4^GMR.PF (Clark et al., 2002) ommatidium, with Scer\GAL4^hs.2sev (Clark et al., 2002) wing, with Scer\GAL4^Eh.2.4 (Clark et al., 2002) wing, with Scer\GAL4^HD23A (Clark et al., 2002) wing, with Scer\GAL4^HD44A (Clark et al., 2002) wing, with Scer\GAL4^hs.2sev (Clark et al., 2002) wing, with Scer\GAL4^sca-109-68 (Clark et al., 2002)
Detailed Description
	Statement Reference Expression of nos^{αTub84B.3'UTR.nos+2.Scer\UAS} in the dorsal pouch epithelium, under the control of Scer\GAL4^HD34A, causes lethality in 25% of flies. (Duchow et al., 2005) Expression of nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^8-123 results in a reduction in the amount of high-order dendritic branches in class IV dendritic arborization neurons of third instar larvae, but the phenotype is significantly less severe than that seen when nos^{αTub84B.3'UTR.Scer\UAS} is expressed under the control of Scer\GAL4^8-123. (Ye et al., 2004) 100% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^GMR.PF have rough eyes. These flies have black spots. 100% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^hs.2sev have rough eyes. Flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^elav.PLu show 84% lethality. Flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4¹⁴⁰⁷ show 61% lethality. 24% of flies expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^hs.2sev show an "adolescent" phenotype; the wing expansion and body cuticle hardening that normally occurs shortly after eclosion fails to occur. The flies are fertile, but show reduced viability. A number of other Scer\GAL4 lines produce this "adolescent" phenotype in combination with nos^{αTub84B.3'UTR.nos+2.Scer\UAS}, they are: Scer\GAL4^sca-109-68 (23% penetrance), Scer\GAL4^HD23A (33% penetrance), Scer\GAL4^HD44A (24% penetrance) and Scer\GAL4^Eh.2.4 (75% penetrance). (Clark et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement Reference Scer\GAL4^sca-109-68, nos^{αTub84B.3'UTR.nos+2.Scer\UAS} has adult cuticle phenotype, non-suppressible by smg¹ (Clark et al., 2002) Scer\GAL4^sca-109-68, nos^{αTub84B.3'UTR.nos+2.Scer\UAS} has wing phenotype, non-suppressible by smg¹ (Clark et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The frequency of the adolescent phenotype in animals expressing nos^{αTub84B.3'UTR.nos+2.Scer\UAS} under the control of Scer\GAL4^sca-109-68 is not altered if they are also carrying smg¹. (Clark et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	nos^{αTub84B.3'UTR.nos+2.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Duchow et al., 2005, Dev. Biol. 282(1): 207--217 The nanos translational control element represses translation in somatic cells by a Bearded box-like motif. [FBrf0187383] Ye et al., 2004, Curr. Biol. 14(4): 314--321 Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons. [FBrf0174478] Clark et al., 2002, Development 129(14): 3325--3334 A common translational control mechanism functions in axial patterning and neuroendocrine signaling in Drosophila. [FBrf0151259]

Allele Dmel\nosαTub84B.3'UTR.nos+2.Scer\UAS

Allele Dmel\nos^{αTub84B.3'UTR.nos+2.Scer\UAS}