Allele Dmel\neur^Scer\UAS.cPa

General Information
Symbol	Dmel\neur^Scer\UAS.cPa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Pavlopoulos	FlyBase ID	FBal0141022
Feature type	allele	Associated gene	Dmel\neur

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Klueg et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of neur. (Klueg et al., 2002) Expression of nucleotides 247-2637 of the neur cDNA (includes 30 bp of 5'UTR and 95 bp of 3'UTR) is under the control of Scer\UAS sequences. (Pavlopoulos et al., 2001)
Carried in construct	P{UAS-neur.P} (Pitsouli and Delidakis, 2005, Pavlopoulos et al., 2001, Le Borgne et al., 2005, Parks and Muskavitch, 2009.2.3, Babaoglan et al., 2009, Nguyen et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference Scer\GAL4^pros.PMG, Dl^Scer\UAS.cLa, neur^Scer\UAS.cPa is a non-suppressor of neuroanatomy defective phenotype of spdo^G104 (Babaoglan et al., 2009)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^Ser.PGF/neur^Scer\UAS.cPa is a suppressor of wing disc phenotype of mib1^EY97600/mib1³ (Le Borgne et al., 2005) Scer\GAL4^twi.PG, Dl^Scer\UAS.cLa, neur^Scer\UAS.cPa is a suppressor of embryonic pericardial cell phenotype of spdo^G104 (Babaoglan et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^kirre-rP298/neur^Scer\UAS.cPa is a non-suppressor of embryonic/larval somatic muscle \| embryonic stage 16 phenotype of mib2¹ (Nguyen et al., 2007) Scer\GAL4^pros.PMG, Dl^Scer\UAS.cLa, neur^Scer\UAS.cPa is a non-suppressor of dMP2 neuron \| ectopic phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^pros.PMG, Dl^Scer\UAS.cLa, neur^Scer\UAS.cPa is a non-suppressor of embryonic pericardial cell phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^pros.PMG, Dl^Scer\UAS.cLa, neur^Scer\UAS.cPa is a non-suppressor of vMP2 neuron phenotype of spdo^G104 (Babaoglan et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of neur[Scer\UAS.cPa] and Dl[Scer\UAS.cLa] under the control of Scer\GAL4[twi.PG] has no effect on eve-positive pericardial cell numbers. Expression of neur[Scer\UAS.cPa] and Dl[Scer\UAS.cLa] under the control of Scer\GAL4[twi.PG] in a spdo[G104] mutant background does not alter the number of eve-positive pericardial cells compared to spdo[G104] mutant embryos. Co-expression of neur[Scer\UAS.cPa] and Dl[Scer\UAS.cLa] under the control of Scer\GAL4[twi.PG] in a spdo[G104] mutant background partially restore svp-positive pericardial cell development by promoting more asymmetric divisions of progenitor cells. (Babaoglan et al., 2009) The muscle defects seen in stage 16 homozygous mib2[1] embryos are not rescued by expression of neur[Scer\UAS.cPa] under the control of Scer\GAL4[kirre-rP298]. (Nguyen et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	26688 y¹ w^67c23; P{UAS-neur.P}2
Notes on Origin
Discoverer

Comments
	Carried in plasmid "pUAST:NeurWT", transfected into S2 cells and used in cell-cell aggregation assays. (Klueg et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	neur^Scer\UAS.cPa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Pavlopoulos (Klueg et al., 2002)
Secondary FlyBase IDs

References ( 7 )
Research paper	Babaoglan et al., 2009, Development 136(24): 4089--4098 Sanpodo: a context-dependent activator and inhibitor of Notch signaling during asymmetric divisions. [FBrf0209326] Nguyen et al., 2007, J. Cell Biol. 179(2): 219--227 Drosophila mind bomb2 is required for maintaining muscle integrity and survival. [FBrf0201389] Le Borgne et al., 2005, PLoS Biol. 3(4): e96 Two distinct E3 ubiquitin ligases have complementary functions in the regulation of Delta and Serrate signaling in Drosophila. [FBrf0184230] Pitsouli and Delidakis, 2005, Development 132(18): 4041--4050 The interplay between DSL proteins and ubiquitin ligases in Notch signaling. [FBrf0187479] Klueg et al., 2002, genesis 34(1-2): 119--122 Creation of a GAL4/UAS-coupled inducible gene expression system for use in Drosophila cultured cell lines. [FBrf0152360] Pavlopoulos et al., 2001, Dev. Cell 1(6): 807--816 neuralized encodes a peripheral membrane protein involved in delta signaling and endocytosis. [FBrf0144791]
Personal communication to FlyBase	Parks and Muskavitch, 2009.2.3, P{UAS-neur.P}2 and P{UAS-neur.DeltaRING::EGFP}8A. P{UAS-neur.P}2 and P{UAS-neur.DeltaRING::EGFP}8A. [FBrf0206636]

Allele Dmel\neurScer\UAS.cPa

Allele Dmel\neur^Scer\UAS.cPa