FlyBase Allele Report: Dmel\Lkb1[4B1-11]

General Information

Symbol

Dmel\Lkb1^4B1-11

Species

D. melanogaster

Name

FlyBase ID

FBal0141053

Feature type

allele

Associated gene

Dmel\Lkb1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Martin and St. Johnston, 2003, Martin et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q98term.

(Martin and St. Johnston, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:13,677,407..13,677,407 [+]

Nucleotide change:

C13677407T

Amino acid change:

Reported amino acid change:

Q98term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator.

(Martin and St. Johnston, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage | somatic clone

(Herzmann et al., 2017)

lethal (with Lkb1^4A4-2)

(Martin and St. Johnston, 2003, Martin et al., 2003)

lethal | embryonic stage | germline clone | maternal effect

(Martin and St. Johnston, 2003)

lethal | recessive

(Martin and St. Johnston, 2003)

Phenotype Manifest In

border follicle cell

(McDonald et al., 2008)

dendrite | pupal stage | somatic clone

(Herzmann et al., 2017)

follicle cell (with Lkb1^4A4-2)

(Martin and St. Johnston, 2003)

larval multidendritic class IV neuron | pupal stage | somatic clone

(Herzmann et al., 2017)

oocyte & microtubule | germ-line clone

(Martin and St. Johnston, 2003)

oocyte | germline clone

(Martin and St. Johnston, 2003)

Detailed Description

Statement

Reference

Lkb1^4B1-11 mutant class IV dendritic arborizing neuron MARCM clones display mild pruning defects at 18 h after pupariation.

(Herzmann et al., 2017)

Mutant third instar larvae show normal clearance of dyed food from the gut.

(Bland et al., 2010)

Heterozygous females have a mild border follicle cell migration defect.

(McDonald et al., 2008)

lkb1^4B1-11 germ line clones cause loss of anterior-posterior polarisation of the oocyte. bcd and osk mRNA localisation fails. Mutant oocytes show loss of anterior-posterior microtubule gradient, with a high density of microtubules round the cortex and the lowest concentration in the centre. The progeny of these females are developmentally arrested before cellularization. A proportion of the mutant oocytes exit meiosis and adopt a nurse cell fate. The penetrance of this phenotype increases with age. This may be due to perdurance of the lkb1 gene product in germ-line clones.

(Martin and St. Johnston, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Lkb1^4B1-11/lkb1[+] is a suppressor of lethal | pupal stage phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^elav-C155

(Schulte et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Lkb1^4B1-11/lkb1[+] is an enhancer of border follicle cell phenotype of Scer\GAL4^slbo.2.6, par-1^UAS.C*.GFP

(McDonald et al., 2008)

Lkb1^4B1-11 is an enhancer of embryonic abdominal segment | maternal effect phenotype of par-1^k06821b/par-1^W3

(Martin and St Johnston, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

lkb1^4B1-11 heterozygous animals expressing Hsap\HTT^{Q138.Scer\UAS.T:Disc\RFP-mRFP} pan-neuronally (under the control of Scer\GAL4^elav-C155) exhibit an adult escaper frequency of 1.8% and 3.7% respectively. These animals are viable and have a relatively normal walking ability, although they do not inflate their wings.

(Schulte et al., 2011)

lkb1^4B1-11/+ significantly enhances the border follicle cell migration defects caused by expression of par-1^{Scer\UAS.C*.T:Avic\GFP} under the control of Scer\GAL4^slbo.2.6.

(McDonald et al., 2008)

The maternal effect embryonic phenotype of par-1^k06821b/par-1^W3 is enhanced by heterozygosity for lkb1^4B1-11.

(Martin and St Johnston, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lkb1^4A4-2

(Martin and St. Johnston, 2003)

Rescued by

Lkb1^4B1-11/Lkb1^4A4-2 is rescued by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^arm.PS

(Martin and St. Johnston, 2003)

Partially rescued by

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{S535A.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Scer\GAL4^arm.PS/Lkb1^{S535A.UASp.GFP}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{C537A.UASp.GFP}/Scer\GAL4^arm.PS

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{C537A.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Scer\GAL4^arm.PS/Lkb1^UASp.GFP

(Martin and St. Johnston, 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80973

w^*; Lkb1^4B1-11/TM6B, Tb¹

Notes on Origin

Discoverer

lkb1^4B1-11 was identified in a genetic screen for germ-line clone mutants that disrupt the localization of the product of the transgene stau^{αTub67C.T:Avic\GFP-m6} in the oocyte.

(Martin and St. Johnston, 2003)

Selected as: a mutant that results in defective localisation of an Avic\GFP-stau marker in living oocytes in females containing homozygous germ-line clones.

(Martin et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lkb1^4B1-11

(Rackley et al., 2021)

lkb1^4B1-11

(Schulte et al., 2011, Bland et al., 2010, McDonald et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close