FlyBase Allele Report: Dmel\Lkb1[4A4-2]

General Information

Symbol

Dmel\Lkb1^4A4-2

Species

D. melanogaster

Name

FlyBase ID

FBal0141054

Feature type

allele

Associated gene

Dmel\Lkb1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Martin and St. Johnston, 2003, Martin et al., 2003)

Progenitor genotype

Cytology

Description

Deletion of M1 to Y145.

(Martin and St. Johnston, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:13,676,960..13,677,549 [+]

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement: lkb^14A4-2 contains a 589-bp deletion (associated with a 12-bp insertion) removing 150 bp of the 5' untranslated region, the start codon and the beginning of the ORF

(Martin and St. Johnston, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA with Ras85D^V12.UAS

(Rackley et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Schulte et al., 2011)

exacerbates carcinoma

modeled by Lkb1^4A4-2, Ras85D^V12.UAS

(Rackley et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell | somatic clone

(McDonald et al., 2008)

centrosome

(Andersen et al., 2012)

dendrite | pupal stage | somatic clone

(Herzmann et al., 2017)

follicle cell (with Lkb1^4B1-11)

(Martin and St. Johnston, 2003)

follicle cell | somatic clone

(Martin and St. Johnston, 2003)

larval brain

(Lee et al., 2006)

larval multidendritic class IV neuron | pupal stage | somatic clone

(Herzmann et al., 2017)

larval ventral nerve cord

(Lee et al., 2006)

neuroblast

(Andersen et al., 2012)

oocyte & microtubule | germ-line clone

(Martin and St. Johnston, 2003)

oocyte | germline clone

(Martin and St. Johnston, 2003)

spindle

(Andersen et al., 2012)

Detailed Description

Statement

Reference

Lkb1^4A4-2 mutant class IV dendritic arborizing neuron MARCM clones display mild pruning defects at 18 h after pupariation.

(Herzmann et al., 2017)

lkb1^4A4-2 mutants exhibit defects in neuroblast apical polarity (in baz and raps localisation), although scrib localisation is as in wild-type. lkb1^4A4-2 mutants exhibit slower cell cycle progression, cytokinesis failure, polyploidy, multiple centrosomes, and malformed mitotic spindles.

(Andersen et al., 2012)

Border follicle cell detachment and migration are disrupted in mosaic egg chambers containing homozygous follicle cell clones; either no border cell migration is seen, or it is incomplete. When the border cells do not complete their migration, border cells and anterior stretched follicle cells are mutant. Mutant border cells are closely associated with mutant anterior stretched follicle cells when the clusters do not detach from the anterior of the egg chamber.

(McDonald et al., 2008)

lkb1^4A4-2 mutants die before the mid-pupal stage.

Homozygous lkb1^4A4-2 mutant larvae exhibit dramatically enlarged brain hemispheres and ventral ganglion, compared to wild-type.

(Lee et al., 2006)

lkb1^4A4-2 germ line clones cause loss of anterior-posterior polarisation of the oocyte. bcd and osk mRNA localisation fails. Mutant oocytes show loss of anterior-posterior microtubule gradient, with a high density of microtubules round the cortex and the lowest concentration in the centre. The progeny of these females are developmentally arrested before cellularization. A proportion of the mutant oocytes exit meiosis and adopt a nurse cell fate. The penetrance of this phenotype increases with age, suggesting perdurance of the lkb1 gene product in germ-line clones. Somatic clones in the ovarian follicle cells that are mutant for lkb1^4A4-2 show defects in polarity. In severely affected clones, the follicular monolayer is disorganized, with mutant cells rounding up and sorting out of the epithelium. This phenotype is penetrant in large stem-cell clones but not in small clones.

(Martin and St. Johnston, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

lkb1[+]/Lkb1^4A4-2 is a non-enhancer of abnormal neuroanatomy phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Suppressor of

Statement

Reference

lkb1[+]/Lkb1^4A4-2 is a suppressor of lethal | pupal stage phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^elav-C155

(Schulte et al., 2011)

lkb1[+]/Lkb1^4A4-2 is a suppressor of abnormal locomotor rhythm phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^elav-C155

(Schulte et al., 2011)

NOT Suppressor of

Statement

Reference

lkb1[+]/Lkb1^4A4-2 is a non-suppressor of abnormal neuroanatomy phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Lkb1^4A4-2 is an enhancer of embryonic abdominal segment | maternal effect phenotype of par-1^k06821b/par-1^W3

(Martin and St Johnston, 2003)

NOT Enhancer of

Statement

Reference

lkb1[+]/Lkb1^4A4-2 is a non-enhancer of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

NOT Suppressor of

Statement

Reference

lkb1[+]/Lkb1^4A4-2 is a non-suppressor of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

lkb1^4A4-2 heterozygous animals expressing Hsap\HTT^{Q138.Scer\UAS.T:Disc\RFP-mRFP} pan-neuronally (under the control of Scer\GAL4^elav-C155) exhibit an adult escaper frequency of 1.8% and 3.7% respectively. These animals are viable and have a relatively normal walking ability, although they do not inflate their wings.

The introduction of an lkb1^4A4-2 heterozygous background enhances the climbing ability of flies expressing Hsap\HTT^{Q138.Scer\UAS.T:Disc\RFP-mRFP} under the control of Scer\GAL4^elav-C155. However, this has no effect on controls.

(Schulte et al., 2011)

Heterozygosity for lkb1^4A4-2 has no effect on the dopaminergic neuronal phenotypes in animals expressing Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^ple.PF.

(Gehrke et al., 2010)

The maternal effect embryonic phenotype of par-1^k06821b/par-1^W3 is enhanced by heterozygosity for lkb1^4A4-2.

(Martin and St. Johnston, 2003, Martin and St Johnston, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lkb1^4B1-11

(Martin and St. Johnston, 2003)

Rescued by

Lkb1^4A4-2 is rescued by Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is rescued by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^arm.PS

(Martin and St. Johnston, 2003)

Lkb1^4A4-2 is rescued by Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4A4-2 is rescued by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^arm.PS

(Martin and St. Johnston, 2003)

Lkb1^4A4-2 is rescued by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4A4-2 is rescued by Scer\GAL4^arm.PS/Lkb1^UASp.GFP

(Martin and St. Johnston, 2003)

Partially rescued by

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{S535A.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Scer\GAL4^arm.PS/Lkb1^{S535A.UASp.GFP}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{C537A.UASp.GFP}/Scer\GAL4^arm.PS

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Lkb1^{C537A.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Lkb1^4B1-11/Lkb1^4A4-2 is partially rescued by Scer\GAL4^arm.PS/Lkb1^UASp.GFP

(Martin and St. Johnston, 2003)

Not rescued by

Lkb1^4A4-2 is not rescued by Lkb1^{K147M.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St Johnston, 2003)

Lkb1^4A4-2 is not rescued by Lkb1^{K147M.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St. Johnston, 2003)

Comments

lkb1^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the loss of anterior-posterior polarity seen in lkb1^4A4-2 germ line clone oocytes. lkb1^{K147M.Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not rescue the loss of anterior-posterior polarity seen in lkb1^4A4-2 germ line clone oocytes.

(Martin and St Johnston, 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80971

w^*; Lkb1^4A4-2/TM6B, Tb¹

Notes on Origin

Discoverer

lkb1^4A4-2 was identified in a genetic screen for germ-line clone mutants that disrupt the localization of the product of the transgene stau^{αTub67C.T:Avic\GFP-m6} in the oocyte.

(Martin and St. Johnston, 2003)

Selected as: a mutant that results in defective localisation of an Avic\GFP-stau marker in living oocytes in females containing homozygous germ-line clones.

(Martin et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

LKB1^4A4-2

(Lee et al., 2006)

Lkb1^4A4-2

(Rackley et al., 2021, Couderc et al., 2017)

lkb1^4A4-2

(Herzmann et al., 2017, Gailite et al., 2015, Andersen et al., 2012, Schulte et al., 2011, McDonald et al., 2008)

lkb1^4A

(Gehrke et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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