FlyBase Allele Report: Dmel\Cul1[EX]

General Information

Symbol

Dmel\Cul1^EX

Species

D. melanogaster

Name

FlyBase ID

FBal0141058

Feature type

allele

Associated gene

Dmel\Cul1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Ou et al., 2002)

Mutagen

Progenitor genotype

Cytology

Description

Imprecise excision of a P-element insertion, resulting in a deletion that removes sequences encoding lin19 amino acids 1 to 90.

(Ou et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Cul1^EX/Cul1^EY11668 transheterozygosity leads to supernumerary type II neuroblasts in the larval optic lobe.

(Komori et al., 2018)

Adult flies carrying Cul1^EX mutant clones in the wings have missing wing veins.

(Suisse et al., 2017)

All lin19^EX mutant ddaC neuron clones exhibit severe dendrite pruning defects at 16 hours after puparium formation. No difference is seen at the white prepupal stage. lin19^EX mutant clones in ddaD/E neurons also fail to prune their larval dendrites at 18 hours after puparium formation. Unlike in wild type lin19^EX mutant ddaF clones survive until 16 hours after puparium formation.

lin19^EX mutant neuroblast clones result in mushroom body γ neurons retain many larval axons at 24 hours after puparium formation that are pruned in wild type. These axons persist into the adult brain. In wandering third instar larvae the dorsal and medial axons are projected normally but the axon branches are less dense than in wild type. All lin19^EX mutant neuroblast clones lack late-born α/β neurons.

(Wong et al., 2013)

lin19^EX mutant clones in the third instar larval wing disc are very small and rarely observed.

(Roberts et al., 2012)

Homozygotes and lin19^EX/Df(2R)CA53 animals die as second instar larvae.

(Ou et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cul1^EX/Cul1^EY11668 has increased cell number | larval stage phenotype, enhanceable by insb^exA45

(Komori et al., 2018)

NOT Enhanced by

Statement

Reference

Cul1^EX has abnormal neuroanatomy | pupal stage | somatic clone phenotype, non-enhanceable by Sox14^Δ13

(Wong et al., 2013)

Suppressed by

Statement

Reference

Cul1^EX has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Cul1^EX has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by Thor^{T37A.T46A.UAS}/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Enhancer of

Statement

Reference

Cul1^EX/Cul1[+] is an enhancer of abnormal neuroanatomy | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Cul1^EX/Cul1[+] is an enhancer of abnormal size | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

NOT Enhancer of

Statement

Reference

Cul1^EX is a non-enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of Sox14^Δ13

(Wong et al., 2013)

Suppressor of

Statement

Reference

Cul1^EX/Cul1[+] is a suppressor of decreased occurrence of cell division | somatic clone | larval stage phenotype of COX5A^tend

(Mandal et al., 2010)

Other

Statement

Reference

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has visible phenotype

(Kim et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cul1^EX/Cul1^EY11668 has type II neuroblast | larval stage | increased number phenotype, enhanceable by insb^exA45

(Komori et al., 2018)

Cul1^EX/Cul1^EY11668 has embryonic/larval optic lobe | larval stage phenotype, enhanceable by insb^exA45

(Komori et al., 2018)

NOT Enhanced by

Statement

Reference

Cul1^EX has larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype, non-enhanceable by Sox14^Δ13

(Wong et al., 2013)

Suppressed by

Statement

Reference

Cul1^EX has larval dorsal multidendritic neuron ddaC | somatic clone | pupal stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Cul1^EX has larval dorsal multidendritic neuron ddaC | somatic clone | pupal stage phenotype, suppressible by Thor^{T37A.T46A.UAS}/Scer\GAL4^ppk.PG

(Wong et al., 2013)

Enhancer of

Statement

Reference

Cul1^EX/Cul1[+] is an enhancer of dendritic tree | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

Cul1^EX/Cul1[+] is an enhancer of larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype of Df(3R)Exel7310/prd1^M56

(Zong et al., 2018)

NOT Enhancer of

Statement

Reference

Cul1^EX is a non-enhancer of larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone phenotype of Sox14^Δ13

(Wong et al., 2013)

Suppressor of

Statement

Reference

Cul1^EX/lin19[+] is a suppressor of eye | somatic clone phenotype of COX5A^tend

(Mandal et al., 2010)

Other

Statement

Reference

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has macrochaeta phenotype

(Kim et al., 2007)

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has scutellum phenotype

(Kim et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Cul1^EX heterozygosity does not significantly affect the ddaC neuron pruning defects observed in prd1^M56/Df(3R)Exel7310 transheterozygous at 16h after puparium formation, namely the length of the persisting dendrites.

(Zong et al., 2018)

Sox14^Δ13 lin19^EX double mutant ddaC neuron clones have similar dendrite pruning defects at 16 hours after puparium formation as are seen in either single mutant alone.

Expression of InR^{K1409A.Scer\UAS} under the control of Scer\GAL4^ppk.PG fully strongly suppresses the dendrite pruning defects seen in lin19^EX mutant ddaC neuron clones at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

Expression of Thor^{T37A.T46A.Scer\UAS} under the control of Scer\GAL4^ppk.PG fully strongly suppresses the dendrite pruning defects seen in lin19^EX mutant ddaC neuron clones at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

(Wong et al., 2013)

A lin19^EX heterozygous background does not suppress the disruptions in cell division seen in CoVa^tend mutant eye disc clones.

(Mandal et al., 2010)

Expression of one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 in a lin19^EX/+ background results in loss of thoracic microchaetae.

(Kim et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cul1^EX is rescued by Scer\GAL4^ppk.PG/Cul1^UAS.Tag:FLAG

(Wong et al., 2013)

Comments

Expression of lin19^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ppk.PG fully rescues the dendrite pruning defects seen in lin19^EX mutant ddaC neuron clones at 16 hours after puparium formation.

(Wong et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Cul1^EX

(Suisse et al., 2017, Rencus-Lazar et al., 2008, Wu et al., 2005, Ou et al., 2002)

Cul1^Ex

(Zong et al., 2018, Roberts et al., 2012, Kim et al., 2007)

cul-1^EX

(Christiansen et al., 2013)

cul1^EX

(Suisse and Treisman, 2019, Cho et al., 2015, Wong et al., 2013, Mandal et al., 2010)

cul1^Ex

(Komori et al., 2018)

lin19^EX

Name Synonyms

Secondary FlyBase IDs

References (13)

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