FlyBase Allele Report: Dmel\fne[UAS.cCa]

General Information

Symbol

Dmel\fne^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chalvet

FlyBase ID

FBal0141115

Feature type

allele

Associated gene

Dmel\fne

Associated Insertion(s)

Carried in Construct

P{UAS-fne.C}

Key Links

Transgenic product class

wild_type

(Chalvet and Samson, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chalvet and Samson, 2002)

Mutagen

in vitro construct

(Chalvet and Samson, 2002)

Progenitor genotype

Carried in construct

P{UAS-fne.C}

Cytology

Description

UASt regulatory sequences drive expression of a 1.5kb EcoRI fragment containing the fne open reading frame plus a 58bp 5' UTR and a 313bp 3' UTR.

(Chalvet and Samson, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

fne

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\MAPT^V337M.UAS

(Shulman et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | oogenesis, with Scer\GAL4^NP1624

(Alizzi et al., 2020)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

increased cell death | oogenesis, with Scer\GAL4^NP1624

(Alizzi et al., 2020)

lethal, with Scer\GAL4^elav-C155

(Chalvet and Samson, 2002)

lethal | larval stage, with Scer\GAL4^elav.PLu

(Samson and Chalvet, 2003)

lethal | larval stage, with Scer\GAL4^elav-C155

(Samson and Chalvet, 2003)

lethal | pupal stage, with Scer\GAL4^elav.PLu

(Samson and Chalvet, 2003)

lethal | pupal stage, with Scer\GAL4^elav-C155

(Samson and Chalvet, 2003)

Phenotype Manifest In

dendritic branch | larval stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

dendritic branch | pupal stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

dendritic tree | larval stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

dendritic tree | pupal stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

follicle cell, with Scer\GAL4^NP1624

(Alizzi et al., 2020)

larval multidendritic class IV neuron | larval stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

larval multidendritic class IV neuron | pupal stage, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

microtubule, with Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

nurse cell, with Scer\GAL4^NP1624

(Alizzi et al., 2020)

prothoracic leg | ectopic, with Scer\GAL4^elav-C155

(Chalvet and Samson, 2002)

wing vein | ectopic, with Scer\GAL4^elav-C155

(Chalvet and Samson, 2002)

Detailed Description

Statement

Reference

In individuals expressing fne^UAS.cCa under the control of Scer\GAL4^ppk.PG, L3 class IV da neurons show decreased terminal branch length, reduced branching proximal to the soma, and increased arbor empty space. The dendritic arbor is delayed at 16h after puparium formation. Arbors show an increase in stable microtubules compared to controls.

The expression of fne^UAS.cCa under the control of Scer\GAL4^NP1624 results in nurse cell death at later stages of egg chamber development; and results in the typically cuboidal or columnar follicle cells to flatten, becoming more squamous in shape.

(Alizzi et al., 2020)

Expression of fne^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PU has no effect on adult eye appearance.

(Shulman et al., 2011)

fne^Scer\UAS.cCa; Scer\GAL4^elav.PLu animals have varying degrees of lethality, and of arrested development at pupal stages, depending on the insertions used: 48% of P{UAS-fne.C}4-10B; P{GAL4-elav.L}G1-10 embryos hatch and 63% of first instar larvae reach adulthood. 38% of P{UAS-fne.C}3-7A; P{GAL4-elav.L}G1-9 embryos hatch, but all arrest at second instar and then survive for a further 10-12 days without additional growth or molting. 30% of P{UAS-fne.C}4-10B; P{GAL4-elav.L}G1-9 embryos hatch, but all of these die as larvae, but all arrest at second instar and then survive for a further 10-12 days without additional growth or molting. 45% of P{UAS-fne.C}3-7A; P{GAL4-elav.L}G1-10 embryos hatch, of which 39% pupate and 36% survive to adulthood. 45% of P{UAS-fne.C}4-10B; Scer\GAL4^elav-C155 embryos hatch, of which 43% pupate and 17% survive to adulthood. All these adults are female. 35% of P{UAS-fne.C}3-7A; Scer\GAL4^elav-C155 embryos hatch, of which only 10% pupate and only 2% survive to adulthood. All these adults are female.

(Samson and Chalvet, 2003)

Expression of fne^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 results in extremely decreased viability. Most of the survivors have extra wing veins and one or two additional partial or full prothoracic legs.

(Chalvet and Samson, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, fne^UAS.cCa has abnormal neuroanatomy | larval stage phenotype, suppressible by Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Enhancer of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

NOT Enhancer of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

NOT Suppressor of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, fne^UAS.cCa has larval multidendritic class IV neuron | larval stage phenotype, suppressible by Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Scer\GAL4^ppk.PG, fne^UAS.cCa has dendritic branch | larval stage phenotype, suppressible by Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Scer\GAL4^ppk.PG, fne^UAS.cCa has dendritic tree | larval stage phenotype, suppressible by Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Scer\GAL4^ppk.PG, fne^UAS.cCa has microtubule phenotype, suppressible | partially by Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Enhancer of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

NOT Enhancer of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-enhancer of dendritic branch | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-enhancer of dendritic tree | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Suppressor of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^ppk.PG is a suppressor of microtubule | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

NOT Suppressor of

Statement

Reference

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-suppressor of larval multidendritic class IV neuron | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-suppressor of dendritic branch | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

fne^UAS.cCa, Scer\GAL4^ppk.PG is a non-suppressor of dendritic tree | larval stage phenotype of Bsg^HMC03195, Scer\GAL4^ppk.PG

(Alizzi et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4^GMR.PU under the control of Hsap\MAPT^{V337M.Scer\UAS} is significantly worsened by co-expression of fne^Scer\UAS.cCa, exacerbating the rough appearance.

(Shulman et al., 2011)

Complementation and Rescue Data

Rescues

fne^UAS.cCa/Scer\GAL4^ppk.PG rescues fne^KOZ40

(Alizzi et al., 2020)

Fails to rescue

fne^UAS.cCa/Scer\GAL4^ppk.PG fails to rescue fne^HMC03569

(Alizzi et al., 2020)

Comments

Images (0)

Mutant

Wild-type