Allele Dmel\f::nec^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\f::nec^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0141118
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\f::nec

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| RNAi, in vitro construct \| coding region fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Reichhart et al., 2002) in vitro construct \| RNAi (Reichhart et al., 2002) in vitro construct \| coding region fusion (Reichhart et al., 2002)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a 601bp antisense f fragment followed by a 566bp antisense nec fragment, followed by mub^816.i, an 816bp spacer fragment derived from an intron of mub, followed by a 566bp sense nec fragment followed by a 601bp sense f fragment. (Reichhart et al., 2002)
Assay mode	In transgenic Drosophila (intraspecific) (Reichhart et al., 2002)
Carried in construct	P{UAS-f::nec-dsRNA} (Reichhart et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	melanotic necrosis, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002)
Phenotype Manifest In
	wing hair, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002) chaeta, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002) chaeta, with Scer\GAL4^ptc-559.1 (Reichhart et al., 2002) chaeta, with Scer\GAL4^VNC.ap (Reichhart et al., 2002) chaeta, with Scer\GAL4^Dll-md23 (Reichhart et al., 2002) chaeta, with Scer\GAL4^dpp.blk1 (Reichhart et al., 2002)
Detailed Description
	Statement Reference Expression of f::nec^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI gives a null f and a null nec mutant phenotype. Expression of f::nec^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1, Scer\GAL4^VNC.ap, Scer\GAL4^Dll-md23 or Scer\GAL4^dpp.blk1 does not give a nec mutant phenotype, but does result in a f mutant phenotype in bristles and hairs. (Reichhart et al., 2002)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	6775 P{UAS-f::nec-dsRNA}1, w¹¹¹⁸ 6774 w¹¹¹⁸; P{UAS-f::nec-dsRNA}6; P{UAS-f::nec-dsRNA}7
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	f::nec^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Reichhart et al., 2002, genesis 34(1-2): 160--164 Splice-activated UAS hairpin vector gives complete RNAi knockout of single or double target transcripts in Drosophila melanogaster. [FBrf0152370]

Allele Dmel\f::necdsRNA.Scer\UAS

Allele Dmel\f::nec^{dsRNA.Scer\UAS}