Open Close
General Information
Symbol
Dmel\dapUAS.cdNa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of de Nooij
FlyBase ID
FBal0141198
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dap, UAS-dacapo, UAS:Dacapo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of dap.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Scer\GAL4en.PU-mediated expression increases the fraction of wing disc cells in G1 phase and concomitantly decreases the fraction in G2.
Very few ovarioles accumulate more than one follicle stem cell (FSC) expressing Scer\GAL4tub-driven dapScer\UAS.cdNa, indicating a modest FSC defect.
Expression of dapScer\UAS.cdNa under the control of Scer\GAL4en-e16E in the posterior compartment cells of embryos causes these cells to arrest in G1 and prevents their death through apoptosis.
Cells expressing dapScer\UAS.cdNa have longer G1 phases than wild-type, though cell doubling times are not significantly different from wild-type, suggesting their G2 and S phases are shorter.
Ovarian follicle cells in somatic clones of dapScer\UAS.cdNa; Scer\GAL4Act5C.PP after stage 6 of oogenesis have small nuclei and do not incorporate BrdU indicating a lack of S phases.
Expression of dapScer\UAS.cdNa under the control of Scer\GAL4ey.PH results in smaller third larval instar eye discs than normal and eyes which are severely reduced in size. Flies expressing dapScer\UAS.cdNa under the control of Scer\GAL4hs.2sev have wild-type eyes. Expression of dapScer\UAS.cdNa under the control of Scer\GAL4en-e16E results in reduced cell division in the posterior compartment of the wing.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Expression of dapScer\UAS.cdNa completely suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The gross neural morphogenesis defects are also suppressed.
Expression of Scer\GAL4tub-driven dapScer\UAS.cdNa completely suppresses the formation of large transient CycEWX follicle cell clones.
Cells expressing dapScer\UAS.cdNa driven by Scer\GAL4Act5C.PP in a Dpa1/Dpa2 background, divide more slowly than controls.
The addition of dapScer\UAS.cdNa suppresses the overproliferation phenotype seen in scrib1, Ras85DV12.Scer\UAS, Scer\GAL4αTub84B.PL clones.
Xenogenetic Interactions
Statement
Reference
The Scer\GAL4en.PU-driven co-expression of BacA\p35UAS.cHa and dapUAS.cdNa leads to a longer sustained DNA damage response to ionizing ration (assessed by phospho-H2Av immunostaining) in the wing disc posterior compartment as compared to the BacA\p35UAS.cHa single expression or wild-type backgrounds.
Expression of dapScer\UAS.cdNa partially rescues the cell death and R7 photoreceptor loss seen when p53GUS.PB is expressed in third instar larval eye discs under the control of Scer\GAL4GMR.PU. The adult eye ablation phenotype is also partially rescued.
Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa substantially suppresses the Scer\GAL4GMR.PF>Hsap\MAPTS396A.S404A.Scer\UAS-induced eye phenotype. Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa substantially suppresses the Scer\GAL4GMR.PF>Hsap\MAPTT231A.S235A.Scer\UAS-induced eye phenotype. Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa substantially suppresses the Scer\GAL4GMR.PF>Hsap\MAPTS202A.T205A.Scer\UAS-induced eye phenotype. Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa substantially suppresses the Scer\GAL4GMR.PF>Hsap\MAPTScer\UAS.cWa-induced eye phenotype.
The rough-eye phenotype observed in animals expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa. Co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa in adult brains expressing Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav.PLu significantly reduces the number of dying cells observed. Vacuolar degeneration of the lamina is rescued in these animals. Co-expression of dapScer\UAS.cdNa and cdc2E51Q.Scer\UAS in adult brains expressing Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav.PLu significantly reduces the number of dying cells observed. The rough-eye phenotype observed in animals expressing Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa. The rough-eye phenotype observed in animals expressing Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of dapScer\UAS.cdNa and cdc2E51Q.Scer\UAS. The rough-eye phenotype observed in animals expressing Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by co-expression of RhebAV4. This enhancement is blocked by co-expressing dapScer\UAS.cdNa and RbfScer\UAS.cDa. The rough-eye phenotype observed in animals expressing Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4GMR.PF is suppressed by co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa. The rough-eye phenotype observed in animals expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye under the control of Scer\GAL4GMR.PF is not modified by co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
J. de Nooij M.G. Wang I.K. Hariharan
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dapScer\UAS.cdNa
dapUAS.cdNa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of de Nooij
Secondary FlyBase IDs
    References (16)