Allele Dmel\dap^{Scer\UAS.cdNa}

General Information
Symbol	Dmel\dap^{Scer\UAS.cdNa}	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of de Nooij	FlyBase ID	FBal0141198
Feature type	allele	Associated gene	Dmel\dap

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Tseng and Hariharan, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of dap. (Tseng and Hariharan, 2002)
Carried in construct	P{UAS-dap.dN} (Tseng and Hariharan, 2002, Brumby and Richardson, 2003, Reis and Edgar, 2004, Shcherbata et al., 2004, Thomer et al., 2004, Parker, 2006, Wang and Kalderon, 2009, Morris et al., 2006, Khurana et al., 2006, Fan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell death \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) lethal, with Scer\GAL4^ap-md544 (Morris et al., 2006) lethal, with Scer\GAL4^Dll-md23 (Morris et al., 2006) lethal, with Scer\GAL4^T80 (Morris et al., 2006) lethal, with Scer\GAL4^αTub84B.PL (Morris et al., 2006) mitotic cell cycle defective, with Scer\GAL4^Act5C.PP (Reis and Edgar, 2004) mitotic cell cycle defective, with Scer\GAL4^unspecified (Reis and Edgar, 2004) mitotic cell cycle defective \| embryonic stage, with Scer\GAL4^en-e16E (Parker, 2006) mitotic cell cycle defective \| somatic clone, with Scer\GAL4^Act5C.PP (Shcherbata et al., 2004) partially lethal - majority die, with Scer\GAL4^32B (Morris et al., 2006) visible, with Scer\GAL4^en-e16E (Tseng and Hariharan, 2002, Morris et al., 2006) visible, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002, Morris et al., 2006) visible, with Scer\GAL4^GMR.PF (Morris et al., 2006)
Phenotype Manifest In
	embryonic segment \| posterior compartment, with Scer\GAL4^en-e16E (Parker, 2006) eye, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002, Morris et al., 2006) eye, with Scer\GAL4^GMR.PF (Morris et al., 2006) eye disc, with Scer\GAL4^ey.PH (Tseng and Hariharan, 2002) follicle cell & nucleus \| somatic clone, with Scer\GAL4^Act5C.PP (Shcherbata et al., 2004) follicle stem cell \| somatic clone (Wang and Kalderon, 2009) mitotic cell cycle, with Scer\GAL4^Act5C.PP (Reis and Edgar, 2004) mitotic cell cycle, with Scer\GAL4^unspecified (Reis and Edgar, 2004) wing \| posterior compartment, with Scer\GAL4^en-e16E (Tseng and Hariharan, 2002) wing cell, with Scer\GAL4^en-e16E (Morris et al., 2006)
Detailed Description
	Statement Reference Very few ovarioles accumulate more than one follicle stem cell (FSC) expressing Scer\GAL4[tub]-driven dap[Scer\UAS.cdNa], indicating a modest FSC defect. (Wang and Kalderon, 2009) Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^en-e16E in the posterior compartment cells of embryos causes these cells to arrest in G1 and prevents their death through apoptosis. (Parker, 2006) Cells expressing dap^{Scer\UAS.cdNa} have longer G1 phases than wild-type, though cell doubling times are not significantly different from wild-type, suggesting their G2 and S phases are shorter. Cells expressing dap^{Scer\UAS.cdNa} driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, divide more slowly than controls. (Reis and Edgar, 2004) Ovarian follicle cells in somatic clones of dap^{Scer\UAS.cdNa}; Scer\GAL4^Act5C.PP after stage 6 of oogenesis have small nuclei and do not incorporate BrdU indicating a lack of S phases. (Shcherbata et al., 2004) Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^ey.PH results in smaller third larval instar eye discs than normal and eyes which are severely reduced in size. Flies expressing dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^hs.2sev have wild-type eyes. Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^en-e16E results in reduced cell division in the posterior compartment of the wing. (Tseng and Hariharan, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference dap^{Scer\UAS.cdNa}/cdc2^{E51Q.Scer\UAS}, Scer\GAL4^elav.PLu is a suppressor of increased cell death \| conditional phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of increased cell death \| conditional phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of increased cell death \| third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of size defective phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of visible phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) dap^{Scer\UAS.cdNa} is a suppressor of tumorigenic \| somatic clone phenotype of Ras85D^V12.Scer\UAS, Scer\GAL4^αTub84B.PL, scrib¹ (Brumby and Richardson, 2003)
NOT Suppressor of
Statement Reference dap^{Scer\UAS.cdNa}, Scer\GAL4^Act88F.PD is a non-suppressor of visible phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Morris et al., 2006)
Other
Statement Reference Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^{Scer\UAS.cdNa} has mitotic cell cycle defective phenotype (Reis and Edgar, 2004, Reis and Edgar, 2004)
Phenotype Manifest In
Enhancer of
Statement Reference dap^{Scer\UAS.cdNa}/Scer\GAL4^tub.PU is an enhancer of follicle stem cell \| somatic clone phenotype of CycE^WX (Wang and Kalderon, 2009)
Suppressor of
Statement Reference dap^{Scer\UAS.cdNa}, Scer\GAL4^GMR.PF, Rbf^Scer\UAS.cDa is a suppressor of eye \| conditional phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/cdc2^{E51Q.Scer\UAS}, Scer\GAL4^elav.PLu is a suppressor of adult brain \| conditional phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/cdc2^{E51Q.Scer\UAS}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of adult brain \| conditional phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of lamina \| conditional phenotype of Hsap\MAPT^{R406W.Scer\UAS}, Scer\GAL4^elav.PLu (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^E14.Scer\UAS, Scer\GAL4^GMR.PF (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Khurana et al., 2006) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of eye disc \| posterior \| third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) dap^{Scer\UAS.cdNa}/Scer\GAL4^GMR.PU is a suppressor \| partially of photoreceptor cell R7 \| third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB (Fan et al., 2010) Scer\GAL4^GMR.PF, dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa is a suppressor of eye phenotype of Hsap\MAPT^{V337M.Scer\UAS}, Rheb^AV4, Scer\GAL4^GMR.PF (Khurana et al., 2006)
NOT Suppressor of
Statement Reference dap^{Scer\UAS.cdNa}, Scer\GAL4^Act88F.PD is a non-suppressor of wing blade phenotype of E2f^Scer\UAS.cNa, Scer\GAL4^Act88F.PD (Morris et al., 2006) dap^{Scer\UAS.cdNa}/Rbf^Scer\UAS.cDa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Khurana et al., 2006)
Other
Statement Reference Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^{Scer\UAS.cdNa} has mitotic cell cycle phenotype (Reis and Edgar, 2004, Reis and Edgar, 2004)
Additional Comments
Genetic Interactions
Statement Reference Expression of Scer\GAL4[tub]-driven dap[Scer\UAS.cdNa] completely suppresses the formation of large transient CycE[WX] follicle cell clones. (Wang and Kalderon, 2009) The addition of dap^{Scer\UAS.cdNa} suppresses the overproliferation phenotype seen in scrib¹, Ras85D^V12.Scer\UAS, Scer\GAL4^αTub84B.PL clones. (Brumby and Richardson, 2003)
Xenogenetic Interactions
Statement Reference Expression of dap[Scer\UAS.cdNa] partially rescues the cell death and R7 photoreceptor loss seen when p53[GUS.PB] is expressed in third instar larval eye discs under the control of Scer\GAL4[GMR.PU]. The adult eye ablation phenotype is also partially rescued. (Fan et al., 2010) The rough-eye phenotype observed in animals expressing Hsap\MAPT[E14.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is suppressed by co-expression of dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. Co-expression of dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa] in adult brains expressing Hsap\MAPT[R406W.Scer\UAS] under the control of Scer\GAL4[elav.PLu] significantly reduces the number of dying cells observed. Vacuolar degeneration of the lamina is rescued in these animals. Co-expression of dap[Scer\UAS.cdNa] and cdc2[E51Q.Scer\UAS] in adult brains expressing Hsap\MAPT[R406W.Scer\UAS] under the control of Scer\GAL4[elav.PLu] significantly reduces the number of dying cells observed. The rough-eye phenotype observed in animals expressing Hsap\MAPT[V337M.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is suppressed by co-expression of dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. The rough-eye phenotype observed in animals expressing Hsap\MAPT[V337M.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is suppressed by co-expression of dap[Scer\UAS.cdNa] and cdc2[E51Q.Scer\UAS]. The rough-eye phenotype observed in animals expressing Hsap\MAPT[V337M.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is enhanced by co-expression of Rheb[AV4]. This enhancement is blocked by co-expressing dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. The rough-eye phenotype observed in animals expressing Hsap\MAPT[Scer\UAS.cWa] under the control of Scer\GAL4[GMR.PF] is suppressed by co-expression of dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. The rough-eye phenotype observed in animals expressing Hsap\MJD[tr.Q78.Scer\UAS.T:Ivir\HA1] in the eye under the control of Scer\GAL4[GMR.PF] is not modified by co-expression of dap[Scer\UAS.cdNa] and Rbf[Scer\UAS.cDa]. (Khurana et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	J. de Nooij M.G. Wang I.K. Hariharan

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dap^{Scer\UAS.cdNa}
Name Synonym	Saccharomyces cerevisiae UAS construct a of de Nooij (Tseng and Hariharan, 2002)
Secondary FlyBase IDs

References ( 10 )
Research paper	Fan et al., 2010, Cell Death Differ. 17(6): 912--921 Dual roles of Drosophila p53 in cell death and cell differentiation. [FBrf0210741] Wang and Kalderon, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(51): 21701--21706 Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. [FBrf0209757] Khurana et al., 2006, Curr. Biol. 16(3): 230--241 TOR-mediated cell-cycle activation causes neurodegeneration in a Drosophila tauopathy model. [FBrf0190026] Morris et al., 2006, PLoS Genet. 2(11): e147 Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo. [FBrf0194141] Parker, 2006, Curr. Biol. 16(20): 2058--2065 Control of compartment size by an EGF ligand from neighboring cells. [FBrf0193620] Reis and Edgar, 2004, Cell 117(2): 253--264 Negative regulation of dE2F1 by cyclin-dependent kinases controls cell cycle timing. [FBrf0174447] Shcherbata et al., 2004, Development 131(13): 3169--3181 The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. [FBrf0179442] Thomer et al., 2004, Development 131(19): 4807--4818 Drosophila double-parked is sufficient to induce re-replication during development and is regulated by cyclin E/CDK2. [FBrf0180151] Brumby and Richardson, 2003, EMBO J. 22(21): 5769--5779 scribble mutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth in Drosophila. [FBrf0167548] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035]

Allele Dmel\dapScer\UAS.cdNa

Allele Dmel\dap^{Scer\UAS.cdNa}