FlyBase Allele Report: Dmel\Traf4[EP578]

General Information

Symbol

Dmel\Traf4^EP578

Species

D. melanogaster

Name

FlyBase ID

FBal0141340

Feature type

allele

Associated gene

Dmel\Traf4

Associated Insertion(s)

P{EP}Traf4^EP578

Carried in Construct

Also Known As

Traf1^EP578, EP(2)0578, EP(2)578, EP0578

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kuranaga et al., 2002, Tseng and Hariharan, 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}Traf4^EP578

Cytology

Description

A P{EP} element is inserted in the 5' flanking region of Traf1.

(Cha et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:4,371,955..4,371,955 [+]

(Kavi et al., 2015, Mallik and Lakhotia, 2009, Mathew et al., 2009, Maybeck and Röper, 2009, Tiwari and Roy, 2009, Vicente-Crespo et al., 2008, Wang et al., 2006, Cha et al., 2005, Cha et al., 2003, Kuranaga et al., 2002, Tseng and Hariharan, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Berkey et al., 2009)

increased cell death, with Scer\GAL4^GMR.PF

(Cha et al., 2003)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009, Tiwari and Roy, 2009, Cha et al., 2003)

eye disc, with Scer\GAL4^GMR.PF

(Cha et al., 2003)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Cha et al., 2003)

ommatidium, with Scer\GAL4^GMR.PF

(Cha et al., 2003)

Detailed Description

Statement

Reference

Homozygous flies are resistant to V. cholerae infection compared to controls.

(Berkey et al., 2009)

Scer\GAL4^GMR.PF-mediated expression of Traf4^EP578 results in rough eyes with disorganized arrays of ommatidia.

(Mallik and Lakhotia, 2009)

Homozygous Traf4^EP578 mutant embryos appear normal.

(Mathew et al., 2009)

Expression via Scer\GAL4^fkh.PH produces a very strongly penetrant phenotype in the embryonic salivary gland of fewer cells in the glands.

(Maybeck and Röper, 2009)

Expression of Traf4^EP578 under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Tiwari and Roy, 2009)

Overexpression of Traf1^EP578, under the regulation of Scer\GAL4^ap-md544 has no effect on thorax closure.

(Cha et al., 2005)

Expression of Traf1^EP578 under the control of Scer\GAL4^GMR.PF leads to a rough eye phenotype. Eyes have a reduced number of ommatidia, abnormally-shaped photoreceptor cells and an overall reduction in the size of the compound eye. This phenotype is more severe when two copies of Traf1^EP578 are expressed.

The eye imaginal discs from transgenic flies overexpressing Traf1^EP578 under the control of Scer\GAL4^GMR.PF contain a highly increased number of apoptotic cells in the region posterior to the morphogenetic furrow in a gene dosage-dependent manner.

(Cha et al., 2003)

Expression of Traf1^EP578 under the control of Scer\GAL4^ey.PH results in eyes that are somewhat reduced in size. Flies expressing Traf1^EP578 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing Traf1^EP578 under the control of Scer\GAL4^en-e16E have wild-type wings.

(Tseng and Hariharan, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Traf4^EP578 has increased cell death phenotype, suppressible by hep¹

(Cha et al., 2003)

Scer\GAL4^ey.PH, Traf4^EP578 has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

Traf4^EP578/Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Rala^35d

(Balakireva et al., 2006)

Traf4^EP578 is an enhancer of visible phenotype of Rala^G20V.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, enhanceable by bsk^UAS.cBa/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, enhanceable by Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, enhanceable by Tak1^UAS.cTa/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, enhanceable by Tak1^UAS.cTa/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, enhanceable by Tak1^UAS.cTa/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, enhanceable by Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, enhanceable by Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, non-enhanceable by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-enhanceable by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, non-enhanceable by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Traf4^EP578 has mesothoracic cleft | ectopic phenotype, suppressible by Scer\GAL4^ap-md544/park[+]/park^UAS.Tag:MYC

(Cha et al., 2005)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, suppressible by hep¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, suppressible by hep¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, suppressible by hep¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, suppressible by Tak1¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, suppressible by Tak1¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, suppressible by Tak1¹

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye disc phenotype, suppressible by hep¹

(Cha et al., 2003)

Scer\GAL4^ey.PH, Traf4^EP578 has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-suppressible by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye photoreceptor cell phenotype, non-suppressible by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has ommatidium phenotype, non-suppressible by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 has eye phenotype, non-suppressible by Traf6^EP1516/Scer\GAL4^GMR.PF

(Cha et al., 2003)

Enhancer of

Statement

Reference

Traf4^EP578, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Rab11^N124I.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Traf4^EP578/Scer\GAL4^sca-537.4 is an enhancer of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

Traf4^EP578/Scer\GAL4^sca-537.4 is an enhancer of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

Traf4^EP578 is an enhancer of macrochaeta phenotype of Rala^G20V.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Scer\GAL4^GMR.PF, Traf4^EP578 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Scer\GAL4^GMR.PF, Traf4^EP578 is an enhancer of eye photoreceptor cell phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

Suppressor of

Statement

Reference

Traf4^EP578 is a suppressor of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Traf4^EP578 is a suppressor of adult thorax phenotype of Scer\GAL4^ap-md544, park^UAS.Tag:MYC

(Cha et al., 2005)

Traf4^EP578/Scer\GAL4^ap-md544 is a suppressor of mesothoracic cleft | ectopic phenotype of Scer\GAL4^ap-md544, park^UAS.Tag:MYC

(Cha et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced eye phenotype caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Traf4^EP578.

(Tiwari and Roy, 2009)

A Traf4^EP578 mutant background suppresses the moderately rough eye phenotype observed upon expression of mbl^C.Scer\UAS under the control of Scer\GAL4^hs.2sev.

(Vicente-Crespo et al., 2008)

The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of Traf1^EP578.

(Cha et al., 2005)

Coexpression of bsk^Scer\UAS.cBa with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, leads to an increase in the disturbance of the ommatidial array in the compound eye in comparison to the eye phenotype resulting from one copy overexpression of Traf1^EP578. When hep^Scer\UAS.cBa is coexpressed with one copy of Traf1^EP578, driven by Scer\GAL4^GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1^EP578 are expressed. Expression of Traf1^EP578, under the control of Scer\GAL4^GMR.PF, in a hep¹/Y background, results in the recovery of an eye of wild-type appearance. The number of apoptotic cells in eye dsics of this genotype are decreased compared to Scer\GAL4^GMR.PF>Traf1^EP578 discs.

Coexpression of Tak1^Scer\UAS.cTa, with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, an increased eye roughness and reduced eye size compared to expression of just Traf1^EP578. Scer\GAL4^GMR.PF>Traf1^EP578 expression in a Tak1¹ background block the rough-eye phenotype caused by Traf1^EP578 overexpression.

Coexpression of Traf2^EP1516 with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, does not alter the rough-eye phenotype caused by sole overexpression of Traf1^EP578.

(Cha et al., 2003)

The rpr^GMR.PW eye phenotypes are significantly suppressed by heterozygosity for Traf1^EP578.

(Kuranaga et al., 2002)

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of Traf1^EP578 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17285

w¹¹¹⁸; P{EP}Traf4^EP578

Notes on Origin

Discoverer

Comments

Overexpression of Traf1^EP578 under the control of Scer\GAL4^hs.PB following a heat shock leads to an increase in Traf2 transcript levels.

(Cha et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

TRAF1^EP578

(Balakireva et al., 2006)

Traf1^EP578

(Vicente-Crespo et al., 2008)

Traf4^EP578

traf1^EP578

(Berkey et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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