FB2022_02, released March 29, 2022

Allele: Dmel\TorTED.UAS

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General Information
Symbol
Dmel\TorTED.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141342
Feature type
allele
Associated gene
Dmel\Tor
Associated Insertion(s)
Carried in Construct
P{UAS-Tor.TED}
Also Known As
UAS-TORTED, UAS-dTORTED, UAS-Tor-DN, TORTED, UAS-Tor TED, UAS-Tor.TED, TOR DN
Key Links
Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Rusten et al., 2004)
Mutagen
in vitro construct - regulatory fusion
(Hennig and Neufeld, 2002)
in vitro construct - coding region fusion
(Hennig and Neufeld, 2002)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Tor.TED}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of Tor amino acids 1228-1947.

      (Hennig and Neufeld, 2002)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Tor
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal developmental rate, with Scer\GAL4Bx-MS1096

      (Hennig and Neufeld, 2002)

      abnormal developmental rate, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      abnormal developmental rate, with Scer\GAL4ppl.PP

      (Colombani et al., 2003)

      abnormal neuroanatomy, with Scer\GAL4elav-C155

      (Deivasigamani et al., 2014)

      abnormal neuroanatomy | larval stage | somatic clone, with Scer\GAL4ppk.PG

      (Lin et al., 2015)

      abnormal size, with Scer\GAL4en.PU

      (Resnik-Docampo and de Celis, 2011)

      abnormal size, with Scer\GAL4MD-638

      (Resnik-Docampo and de Celis, 2011)

      abnormal size, with Scer\GAL4nub-AC-62

      (Resnik-Docampo and de Celis, 2011)

      decreased body size, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      decreased body size | nutrition conditional, with Scer\GAL4ppl.PP

      (Kamakura, 2011)

      decreased cell number, with Scer\GAL4nub-AC-62

      (Resnik-Docampo and de Celis, 2011)

      decreased cell size, with Scer\GAL4nub-AC-62

      (Resnik-Docampo and de Celis, 2011)

      decreased cell size | somatic clone, with Scer\GAL4Act.PU

      (Mukherjee and Duttaroy, 2013)

      increased cell death, with Scer\GAL4en.PU

      (Resnik-Docampo and de Celis, 2011)

      increased occurrence of cell division | larval stage, with Scer\GAL4en.PU

      (Resnik-Docampo and de Celis, 2011)

      lethal, with Scer\GAL4Act5C.PI

      (Hennig and Neufeld, 2002)

      lethal, with Scer\GAL4ap-md544

      (Hennig and Neufeld, 2002)

      lethal, with Scer\GAL4da.G32

      (Kapahi et al., 2004)

      lethal, with Scer\GAL4ey.PH

      (Hennig and Neufeld, 2002)

      visible, with Scer\GAL4Bx-MS1096

      (Mukherjee and Duttaroy, 2013, Hennig and Neufeld, 2002)

      visible, with Scer\GAL4en-e16E

      (Hennig and Neufeld, 2002)

      wild-type, with Scer\GAL4GMR.PF

      (Hennig and Neufeld, 2002)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Late pupae expressing TorTED.Scer\UAS under the control of Scer\GAL4ninaE.PU and reared in constant light conditions do not present any obvious defects in rhabdomere structure or in endomembranes in photoreceptors, as compared to controls.

      (Kamalesh et al., 2017)

      Scer\GAL4bs.PM-mediated expression of TorTED.Scer\UAS results in decreased terminal cell branching and induction of long, branched autocellular, seamed tubes extend well beyond the terminal cell nucleus.

      (Francis and Ghabrial, 2015)

      TorTED.Scer\UAS-expressing clones (generated using MARCM and Scer\GAL4ppk.PG) show relatively mild dendrite growth defects in the large C4da dendritic arbors.

      (Lin et al., 2015)

      Expression of snamaNIG.3231R under the control of Scer\GAL4elav-C155 marginally increases bouton size compared to controls.

      (Deivasigamani et al., 2014)

      Cells in clones expressing TorTED.Scer\UAS under the control of Scer\GAL4Act.PU in the fat body are much smaller in size than wild-type cells and have tiny nuclei.

      Expression of TorTED.Scer\UAS under the control of Scer\GAL4Bx-MS1096 results in shortened, misshaped wings.

      (Mukherjee and Duttaroy, 2013)

      In contrast to wild-type, transgenic flies expressing Scer\GAL4ppl.PP>TorTED.Scer\UAS and reared on medium supplemented with Apis mellifera royal jelly do not show increased body size. However, similarly to wild-type flies in response to royal jelly, they display shortened developmental time compared with flies reared on control medium.

      (Kamakura, 2011)

      Expression of TorTED.Scer\UAS using Scer\GAL4en.PU results in a significant increase in the fraction of mitotic cells in the posterior compartment of the wing disc.

      Expression of TorWT.Scer\UAS.T:Zzzz\FLAG using Scer\GAL4en.PUinduces only very low levels of apoptosis in the the wing disc.

      Expression of TorTED.Scer\UAS using Scer\GAL4nub-AC-62 results in abnormally small wings in which cell size and cell number are decreased.

      Expression of TorTED.Scer\UAS using Scer\GAL4nub-AC-62, Scer\GAL4en.PU or Scer\GAL4MD-638 results in a severe reduction in wing size.

      (Resnik-Docampo and de Celis, 2011)

      Expression of TorTED.Scer\UAS under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

      (Birse et al., 2010)

      Expression of TorTED.Scer\UAS under the control of Scer\GAL4Lsp2.PH induces autolysosome formation in the fat body.

      (Haberman et al., 2010)

      Expression of TorTED.Scer\UAS in the eye under the control of Scer\GAL4GMR.PU results in retinal degeneration. At 14 days old, degenerating photoreceptor cells display large autophagosomes.

      (Nisoli et al., 2010)

      Animals expressing TorTED.Scer\UAS under the control of Scer\GAL4da.G32 do not eclose to adulthood.

      (Kapahi et al., 2004)

      When expression is driven by Scer\GAL4Lsp2.PH, in the larval fat body, autolysosomes show a marked increase in size (up to 10 microns), a 30% increase in autophagic area, a reduction of cytoplasmic area and disappearance of structures labelled with Hsap\MLP3BScer\UAS.T:Avic\GFP-EGFP alone. Autolysosomal structures are less acidic than in wild type. Fat droplets accumulate, abnormally.

      (Rusten et al., 2004)

      When TorTED.Scer\UAS is driven by Scer\GAL4ppl.PP in the fat body vesicle aggregation and restricted endoreduplication is seen. This is associated with a developmental delay and a weak growth defect.

      (Colombani et al., 2003)

      Expression of TorTED.Scer\UAS under the control of Scer\GAL4Bx-MS1096 results in smaller wings than normal which show an upward curvature. The flies show a developmental delay. Expression of TorTED.Scer\UAS under the control of Scer\GAL4en-e16E results in a developmental delay and a reduced posterior wing.

      (Hennig and Neufeld, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4nub-AC-62/TorTED.UAS is an enhancer of wing phenotype of Scer\GAL4nub-AC-62, hppyUAS.cMa

      (Resnik-Docampo and de Celis, 2011)

      TorTED.UAS, Scer\GAL4GMR.PU is an enhancer of autophagosome phenotype of Gug75QN.UAS, Scer\GAL4GMR.PU

      (Nisoli et al., 2010)

      TorTED.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug75QN.UAS, Scer\GAL4GMR.PU

      (Nisoli et al., 2010)

      TorTED.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of Gug66QC.UAS, Scer\GAL4GMR.PU

      (Nisoli et al., 2010)

      TorTED.UAS, Scer\GAL4GMR.PU is an enhancer of retina | temperature conditional phenotype of GugUAS.cCa, Scer\GAL4GMR.PU

      (Nisoli et al., 2010)
      NOT Enhancer of
      Statement
      Reference

      Scer\GAL4elav-C155/TorTED.UAS is a non-enhancer of eye phenotype of cathD1

      (Kuronen et al., 2009)
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of TorTED.Scer\UAS suppresses the increased midgut cell size resulting from the expression of Atg9HMS01246 under the control of Scer\GAL4Myo31DF-NP0001.

      (Wen et al., 2017)

      Clonal expression of TorTED.Scer\UAS under the control of Scer\GAL4tub.PU fails to suppress the formation of ectopic neuroblasts in larval optic lobe nerfin-1159/nerfin-1159 mutant clones.

      (Xu et al., 2017)

      The late pupal lethality observed in animals expressing AMPKαJF01951 under the control of Scer\GAL4tub.PU can be partially rescued by co-expression of TorTED.Scer\UAS.

      The pupal-stage lethality of either hemizygous AMPKα1 or AMPKα3 males cannot be rescued by expression of TorTED.Scer\UAS under the control of Scer\GAL4tub.PU.

      (Onyenwoke et al., 2015)

      Expression of TorTED.Scer\UAS suppresses the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

      Expression of TorTED.Scer\UAS does not suppress the small bouton phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4elav-C155.

      (Deivasigamani et al., 2014)

      Co-expression of srlEY05931 significantly increases cell size (both nuclear area and cytoplasmic area) in clones in the fat body expressing TorTED.Scer\UAS under the control of Scer\GAL4Act.PU.

      Co-expression of srlEY05931 suppresses the wing defects caused by expression of TorTED.Scer\UAS under the control of Scer\GAL4Bx-MS1096.

      (Mukherjee and Duttaroy, 2013)

      Expression of TorTED.Scer\UAS suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

      (Wong et al., 2013)

      Co-expression of hppyScer\UAS.cMa and TorTED.Scer\UAS using Scer\GAL4nub-AC-62 results in a strong synergistic phenotype of reduced wing size and reduced cell size and number.

      Co-expression of hppyGD12129 significantly rescues the reduced wing size caused by expression of TorTED.Scer\UAS using Scer\GAL4nub-AC-62 or Scer\GAL4MD-638.

      (Resnik-Docampo and de Celis, 2011)

      Expression of TorTED.Scer\UAS under the control of Scer\GAL4Lsp2.PH cannot induce autolysosome formation in the fat body in a Acn1/Acn27 background.

      (Haberman et al., 2010)

      Expression of dominant-negative TorTED.Scer\UAS partially rescues the lethality cause by global expression (Scer\GAL4αTub84B.PL) of SNF1AdsRNA.Scer\UAS.

      (Johnson et al., 2010)

      Co-expression of TorTED.Scer\UAS and Gug75QN.Scer\UAS in the eye under the control of Scer\GAL4GMR.PU results in the formation of gigantic autophagolysosomes.

      Expression of Gug75QN.Scer\UAS under the control of Scer\GAL4GMR.PU in Atg1Δ3D mutant eyes fails to rescue cell degeneration.

      Expression of GugScer\UAS.cCa in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of TorTED.Scer\UAS.

      Expression of Gug66QC.Scer\UAS in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of TorTED.Scer\UAS.

      Expression of Gug75QN.Scer\UAS in the adult eye under the control of Scer\GAL4GMR.PU, aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of TorTED.Scer\UAS.

      (Nisoli et al., 2010)

      Expression of TorTED.Scer\UAS partially suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.

      (Read et al., 2009)

      Co-expression of TorTED.Scer\UAS partially suppresses the salivary gland overgrowth phenotype that is seen in animals expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4fkh.PH and at 24 hours after puparium formation the salivary glands are predominantly degraded in the double mutant animals.

      Co-expression of TorTED.Scer\UAS suppresses the salivary gland overgrowth phenotype that is seen in animals expressing Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4fkh.PH and at 24 hours after puparium formation the salivary glands are predominantly degraded in the double mutant animals.

      (Berry and Baehrecke, 2007)

      The reduced wing phenotype caused by expression of TorTED.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is partially suppressed by dmScer\UAS.cZa.

      (Hennig and Neufeld, 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TorTED.Scer\UAS
      TorTED.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (43)