Allele Dmel\Sir2^4.5

General Information
Symbol	Dmel\Sir2^4.5	Species	D. melanogaster
Name		FlyBase ID	FBal0141417
Feature type	allele	Associated gene	Dmel\Sir2
Also Known As	dSir2^4.5

Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}k14513 (Newman et al., 2002)
Nature of the lesion	Statement Reference Imprecise excision of the P{lacW} element, resulting in a deletion of Sir2 sequences from -16 to +859 (coding sequences of the adjacent gene, DnaJ-H, are intact). (Newman et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (with Sir2^5.26) (Newman et al., 2002) suppressor of variegation (with Sir2^5.26), with In(1)w^m4 (Newman et al., 2002) viable (with Sir2^5.26) (Newman et al., 2002)
Phenotype Manifest In

Detailed Description
	Statement Reference The median lifespan of Sir2[4.5] flies (mutant line generated by outcrossing the mutation into the Canton S background) responds in the same way as that of Canton S controls to a range of diets (from dietary restriction to fully-fed conditions). (Burnett et al., 2011) Sir2[4.5]/Sir2[5.26] males and females have normal lifespans. (Balan et al., 2008) Unlike wild-type flies, the lifespan of Sir2^4.5/Sir2^5.26 flies is note extended by a diet of low-calorie food. (Rogina and Helfand, 2004) Sir2^4.5/Sir2^5.26 flies are phenotypically normal. Under nonstress environmental conditions, the average median life span of Sir2^4.5/Sir2^5.26 males is slightly, but significantly less than that of heterozygous controls. The average life span is not significantly different from controls. Under stress conditions, the mean life span of Sir2^4.5/Sir2^5.26 males is not significantly different from controls. The median life span of Sir2^4.5/Sir2^5.26 males is significantly longer than that of controls. (Newman et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Sir2^4.5/Sir2^5.26 is a suppressor of long lived phenotype of Naam^{Scer\UAS.T:SV5\V5}, Scer\GAL4^tub.PU (Balan et al., 2008)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The increase in lifespan caused by expression of Naam[Scer\UAS.T:SV5\V5] under the control of Scer\GAL4[elav.PLu] is completely suppressed by Sir2[4.5]/Sir2[5.26]. (Balan et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	32568 Sir2^4.5 cn¹/SM6b, P{eve-lacZ8.0}SB1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dSir2^4.5 (Rogina and Helfand, 2004, Bianchi-Frias et al., 2004, Newman et al., 2002, Burnett et al., 2011) l(2)K14513^4.5 (Newman et al., 2002) Sir2^4.5 (Wood et al., 2004, Balan et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Burnett et al., 2011, Nature 477(7365): 482--485 Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila. [FBrf0215823] Balan et al., 2008, J. Biol. Chem. 283(41): 27810--27819 Life span extension and neuronal cell protection by Drosophila nicotinamidase. [FBrf0207070] Bianchi-Frias et al., 2004, PLoS Biol. 2(7): e178 Hairy transcriptional repression targets and cofactor recruitment in Drosophila. [FBrf0179136] Rogina and Helfand, 2004, Proc. Natl. Acad. Sci. U.S.A. 101(45): 15998--16003 Sir2 mediates longevity in the fly through a pathway related to calorie restriction. [FBrf0180651] Wood et al., 2004, Nature 430(7000): 686--689 Sirtuin activators mimic caloric restriction and delay ageing in metazoans. [FBrf0180554] Newman et al., 2002, Genetics 162(4): 1675--1685 A Drosophila homologue of sir2 modifies position-effect variegation but does not affect life span. [FBrf0155506]

Allele Dmel\Sir24.5

Allele Dmel\Sir2^4.5