Sirt14.5/Sirt12A-7-11 adult males show significantly decreased survival under starvation conditions at 3 weeks (but not 2 weeks) old, compared to controls (relative feeding rate is similar to controls at 1 or 2 weeks old). Sirt14.5/Sirt12A-7-11 males fed ad libitum show significantly higher levels of glucose and glycogen (at 1 and 2 weeks old) and triglycerides (at 2 but not 1 weeks old) compared to wild type. In fasting conditions, mutants show significantly higher glucose levels (at 2 but not 1 week old), compared to wild type. Sirt14.5/Sirt12A-7-11 mutants are insulin resistant by 2 weeks of age.
The median lifespan of Sir24.5 flies (mutant line generated by outcrossing the mutation into the Canton S background) responds in the same way as that of Canton S controls to a range of diets (from dietary restriction to fully-fed conditions).
Unlike wild-type flies, the lifespan of Sirt14.5/Sirt15.26 flies is not extended by a diet of low-calorie food.
Sir24.5/Sir25.26 flies are phenotypically normal. Under nonstress environmental conditions, the average median life span of Sir24.5/Sir25.26 males is slightly, but significantly less than that of heterozygous controls. The average life span is not significantly different from controls. Under stress conditions, the mean life span of Sir24.5/Sir25.26 males is not significantly different from controls. The median life span of Sir24.5/Sir25.26 males is significantly longer than that of controls.