Open Close
General Information
Symbol
Dmel\Sirt14.5
Species
D. melanogaster
Name
FlyBase ID
FBal0141417
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dSir24.5, Sir24.5
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    deletion
    Comment:
    A deletion resulting from the imprecise excision of P{PZ}Sirt105327a, removes sequences from -16 to +759 with respect to the Sirt1 transcription start. The start site was mapped to a base that corresponds to the 5' end of EST GB:LD07439.
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Imprecise excision of the P{PZ} element, resulting in a deletion of Sirt1 sequences from -16 to +759 (coding sequences of the adjacent gene, DnaJ-H, are intact).
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    Sirt14.5/Sirt12A-7-11 adult males show significantly decreased survival under starvation conditions at 3 weeks (but not 2 weeks) old, compared to controls (relative feeding rate is similar to controls at 1 or 2 weeks old). Sirt14.5/Sirt12A-7-11 males fed ad libitum show significantly higher levels of glucose and glycogen (at 1 and 2 weeks old) and triglycerides (at 2 but not 1 weeks old) compared to wild type. In fasting conditions, mutants show significantly higher glucose levels (at 2 but not 1 week old), compared to wild type. Sirt14.5/Sirt12A-7-11 mutants are insulin resistant by 2 weeks of age.
    The median lifespan of Sir24.5 flies (mutant line generated by outcrossing the mutation into the Canton S background) responds in the same way as that of Canton S controls to a range of diets (from dietary restriction to fully-fed conditions).
    Sir24.5/Sir25.26 males and females have normal lifespans.
    Unlike wild-type flies, the lifespan of Sirt14.5/Sirt15.26 flies is not extended by a diet of low-calorie food.
    Sir24.5/Sir25.26 flies are phenotypically normal. Under nonstress environmental conditions, the average median life span of Sir24.5/Sir25.26 males is slightly, but significantly less than that of heterozygous controls. The average life span is not significantly different from controls. Under stress conditions, the mean life span of Sir24.5/Sir25.26 males is not significantly different from controls. The median life span of Sir24.5/Sir25.26 males is significantly longer than that of controls.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    NOT Suppressor of
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Two copies of Hnf4T:Avic\GFP-SF,T:Zzzz\FLAG restores normal insulin signaling responses but does not rescue hyperglycemia and elevated glycogen levels in Sirt14.5/Sirt12A-7-11 flies.
    The increase in lifespan caused by expression of NaamScer\UAS.T:SV5\V5 under the control of Scer\GAL4elav.PLu is completely suppressed by Sir24.5/Sir25.26.
    Xenogenetic Interactions
    Statement
    Reference
    Sir24.5/Sir25.26 does not suppress the protective effect on axotomised olfactory receptor neurons in flies expressing Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.8197. No change is seen in the amount of Wallerian degeneration 15 days after injury.
    Complementation and Rescue Data
    Comments
    Expression of Sirt1Scer\UAS.cPa driven in the fat body by Scer\GAL4r4 (but not when driven by Scer\GAL4Mef2.PR or Scer\GAL4Ilp2.215-1) rescues insulin signaling in peripheral tissues of Sirt14.5/Sirt12A-7-11 flies.
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    References (8)