FlyBase Allele Report: Scer\GAL4[ems.HRE]

General Information

Symbol

Scer\GAL4^ems.HRE

Species

S. cerevisiae

Name

FlyBase ID

FBal0141437

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ems-GAL4.HRE}

Also Known As

ems-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Merabet et al., 2002)

Progenitor genotype

Carried in construct

P{ems-GAL4.HRE}

Cytology

Description

The 1.2kb ems Hox response element (HRE) regulates expression of a GAL4 driver.

(Merabet et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

ems

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12

spiracular chamber

(Krattinger et al., 2007)

embryonic stage 12 -- 15

posterior spiracle primordium | restricted

(Merabet et al., 2002)

embryonic stage 15

spiracular chamber

(Tsikala et al., 2014, Merabet et al., 2002)

Additional Information

Statement

Reference

ScerGAL4^ems.HRE first drives expression during germ-band retraction, and is restricted to the cells that will form the filzkorper.

(Merabet et al., 2002)

Marker for

Reflects expression of

ems

(Tsikala et al., 2014, Krattinger et al., 2007, Merabet et al., 2002)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cortical actin cytoskeleton & embryonic posterior spiracle, with Rho1^N19.UAS

(Simões et al., 2006)

embryonic/larval spiracle, with crb^{T6AT9AS11AS13A.intra.UAS}

(Lovegrove et al., 2006)

embryonic/larval spiracle primordium, with Rac1^L89.UAS

(Lovegrove et al., 2006)

embryonic/larval spiracle primordium, with Rac1^V12.UAS

(Lovegrove et al., 2006)

embryonic/larval spiracle primordium, with Rho1^N19.UAS

(Lovegrove et al., 2006)

embryonic/larval spiracle primordium, with Rho1^V14.UAS

(Lovegrove et al., 2006)

filzkorper, with ci^N:Zn.UAS

(Merabet et al., 2005)

filzkorper, with Egfr^DN.UAS.cBa

(Merabet et al., 2005)

filzkorper, with pan^ΔN.UAS

(Merabet et al., 2005)

filzkorper, with Rho1^N19.UAS

(Simões et al., 2006)

filzkorper, with Rho1^V14.UAS

(Simões et al., 2006)

filzkorper, with spi^s.UAS

(Merabet et al., 2005)

filzkorper, with wg^UAS.cLb

(Merabet et al., 2005)

posterior spiracle primordium, with cv-c^UAS.cDa

(Simões et al., 2006)

posterior spiracle primordium, with Rho1^N19.UAS

(Simões et al., 2006)

posterior spiracle primordium, with Rho1^V14.UAS

(Simões et al., 2006)

stigmatophore, with spi^s.UAS

(Merabet et al., 2005)

stigmatophore, with wg^UAS.cLb

(Merabet et al., 2005)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Rho1^N19.UAS, Scer\GAL4^ems.HRE has posterior spiracle primordium phenotype, suppressible by RhoGEF64C^UAS.cBa, Scer\GAL4^ems.HRE

(Simões et al., 2006)

NOT suppressed by

Statement

Reference

Rho1^N19.UAS, Scer\GAL4^ems.HRE has posterior spiracle primordium phenotype, non-suppressible by RhoGEF64C^ΔC.UAS, Scer\GAL4^ems.HRE

(Simões et al., 2006)

Suppressor of

Statement

Reference

RhoGEF64C^UAS.cBa, Scer\GAL4^ems.HRE is a suppressor of posterior spiracle primordium phenotype of Rho1^N19.UAS, Scer\GAL4^ems.HRE

(Simões et al., 2006)

NOT Suppressor of

Statement

Reference

RhoGEF64C^ΔC.UAS, Scer\GAL4^ems.HRE is a non-suppressor of posterior spiracle primordium phenotype of Rho1^N19.UAS, Scer\GAL4^ems.HRE

(Simões et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Btk^UAS.RC/Scer\GAL4^ems.HRE fails to rescue Btk^k00206

(Tsikala et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^ems.HRE

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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