Allele Dmel\S6k^TE.Scer\UAS

General Information
Symbol	Dmel\S6k^TE.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0141551
Feature type	allele	Associated gene	Dmel\S6k

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Barcelo and Stewart, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: T398E. Scer\UAS regulatory sequences drive expression of a mutated form of S6k. (Barcelo and Stewart, 2002)
Carried in construct	P{UAS-S6k.TE} (Barcelo and Stewart, 2002, Scott et al., 2004, Scott et al., 2004, Kockel et al., 2010, Wang et al., 2009, Resnik-Docampo and de Celis, 2011, Shen and Ganetzky, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell size, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) size defective, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) visible, with Scer\GAL4^ap-md544 (Barcelo and Stewart, 2002)
Phenotype Manifest In
	wing, with Scer\GAL4^ap-md544 (Barcelo and Stewart, 2002) wing, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Detailed Description
	Statement Reference Expression of S6k[TE.Scer\UAS] using Scer\GAL4[nub-AC-62] results in abnormally large wings in which cell size is increased. (Resnik-Docampo and de Celis, 2011) Expression of S6k[TE.Scer\UAS] under the control of Scer\GAL4[elav.PLu] has little effect on neuromuscular junction growth. (Shen and Ganetzky, 2009) After 30 days of light/dark cycling, photoreceptors are preserved normally in Scer\GAL4[GMR.PU] driven S6k[TE.Scer\UAS] flies. (Wang et al., 2009) Expression of S6k^TE.Scer\UAS under the control of Scer\GAL4^ap-md544 results in wings which bend down (the wings bend down more than in flies expressing S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544). (Barcelo and Stewart, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference S6k^TE.Scer\UAS, Scer\GAL4^nub-AC-62 is a suppressor of decreased cell size phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011) S6k^TE.Scer\UAS, Scer\GAL4^nub-AC-62 is a suppressor of size defective phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011) Scer\GAL4^αTub84B.PL/S6k^TE.Scer\UAS is a suppressor of cell size defective phenotype of Tor^ΔP (Scott et al., 2004)
NOT Suppressor of
Statement Reference S6k^TE.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of increased cell growth phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) S6k^TE.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuroanatomy defective phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009)
Phenotype Manifest In
Suppressor of
Statement Reference S6k^TE.Scer\UAS, Scer\GAL4^nub-AC-62 is a suppressor of wing phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011)
NOT Suppressor of
Statement Reference S6k^TE.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) S6k^TE.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of S6k[TE.Scer\UAS] diminishes the small wing and decreased wing cell size phenotypes seen in hppy[Scer\UAS.cMa], Scer\GAL4[nub-AC-62] wings. (Resnik-Docampo and de Celis, 2011) Co-expression of S6k[TE.Scer\UAS] under the control of Scer\GAL4[elav.PLu], fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1[Scer\UAS.cSa]. (Shen and Ganetzky, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	6912 w¹¹¹⁸; P{UAS-S6k.TE}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	S6k^TE.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911] Kockel et al., 2010, PLoS Genet. 6(6): e1000990 Dynamic Switch of Negative Feedback Regulation in Drosophila Akt-TOR Signaling. [FBrf0211124] Shen and Ganetzky, 2009, J. Cell Biol. 187(1): 71--79 Autophagy promotes synapse development in Drosophila. [FBrf0208921] Wang et al., 2009, J. Cell Biol. 186(5): 703--711 TOR-mediated autophagy regulates cell death in Drosophila neurodegenerative disease. [FBrf0208794] Scott et al., 2004, Dev. Cell 7(2): 167--178 Role and regulation of starvation-induced autophagy in the Drosophila fat body. [FBrf0180117] Barcelo and Stewart, 2002, genesis 34(1-2): 83--85 Altering Drosophila S6 kinase activity is consistent with a role for S6 kinase in growth. [FBrf0152384]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440]

Allele Dmel\S6kTE.Scer\UAS

Allele Dmel\S6k^TE.Scer\UAS