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General Information
Symbol
Dmel\S6kSTDETE.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141552
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-S6KSTDETE, S6KSTDETE, UAS-S6KCA, UAS-dS6KSTDETE, UAS-S6k.STDETE, UAS-S6K-STDETE
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated form of S6k (carries the amino acid replacements T398E, S418D and T422E).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav-C155 has no effect on bouton size.

Expressing S6kSTDETE.Scer\UAS under the control of Scer\GAL4da.G32 suppresses the increase in sleep duration seen in wild type flies in response to rapamycin.

Cells in clones in the fat body expressing S6kSTDETE.Scer\UAS under the control of Scer\GAL4Act.PU are overgrown.

The expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav.Switch.PO during the last 48 hours of larval development has no effect on quantal content at the larval neuromuscular junction.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4VGlut-OK371 has no effect on bouton size or number at the larval neuromuscular junction.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4AB1 results in a 135% increase average cell size in the salivary gland.

Expression of S6kSTDETE.Scer\UAS driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav.PLu has little effect on neuromuscular junction growth.

Scer\GAL4GMR.long>S6kSTDETE.Scer\UAS mutants show no defects in opsin regulation.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4da.G32 at 29oC results in a decrease in mean life span of 34% compared to control flies.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4ap-md544 results in wings which bend down (the wings bend down more than in flies expressing S6kScer\UAS.cMa under the control of Scer\GAL4ap-md544).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Phenotype Manifest In
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prelScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4109(2)80 is not significantly affected by co-expression of S6kSTDETE.Scer\UAS.

Expression of S6kSTDETE.Scer\UAS does not suppress the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of S6kSTDETE.Scer\UAS suppresses the small bouton phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4elav-C155.

The reduction in the distance between wing veins 3 and 4 and the reduction in cell size in the wing that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is suppressed if they are also co-expressing S6kSTDETE.Scer\UAS.

Co-expression of S6kSTDETE.Scer\UAS rescues the reduced size of clones in third larval instar wing disc and fat body expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4Act5C.PU.

Co-expression of srldsRNA.Scer\UAS.cUa suppresses the cell overgrowth phenotype seen in cells in clones in the fat body expressing S6kSTDETE.Scer\UAS under the control of Scer\GAL4Act.PU.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4VGlut-OK371 partially rescues the reduction in bouton size at the neuromuscular junction which is seen in Pdk133 larvae. In addition, the number of boutons at the neuromuscular junction is increased compared to wild type.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4AB1 in a trpm28 background does not result in an increase in cell size in the larval salivary gland.

Co-expression of Sesnd04539 with S6kSTDETE.Scer\UAS via Scer\GAL4ap-md544 fails to suppress the S6kSTDETE.Scer\UAS hyperplastic wing phenotype.

Sesn8A11 does not enhance the Scer\GAL4ap-md544-driven S6kSTDETE.Scer\UAS-stimulated cell growth in the wing.

Co-expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav.PLu, fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1Scer\UAS.cSa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The decrease in bouton size which is seen at the neuromuscular junction in S6kl-1/Df(3L)CH18 third instar larvae is rescued by expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav-C155 and the boutons are actually increased in size compared to wild type in the rescued animals.

The reduced active zone number and reduced reduced total synaptic area seen at the neuromuscular junction of S6kl-1/Df(3L)CH18 third instar larvae is also rescued by expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4elav-C155 (the numbers are actually increased in size compared to wild type in the rescued animals).

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
S6kSTDETE.Scer\UAS
S6kSTDETE.UAS
Name Synonyms
Secondary FlyBase IDs
    References (20)