FlyBase Allele Report: Dmel\S6k[STDETE.UAS]

General Information

Symbol

Dmel\S6k^STDETE.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0141552

Feature type

allele

Associated gene

Dmel\S6k

Associated Insertion(s)

Carried in Construct

P{UAS-S6k.STDETE}

Also Known As

UAS-S6K^STDETE, UAS-S6K^CA, S6K^STDETE, UAS-dS6K^STDETE, UAS-S6k.STDETE, UAS-S6K-STDETE

Key Links

Transgenic product class

missense_variant

(Barcelo and Stewart, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Barcelo and Stewart, 2002)

Mutagen

in vitro construct

(Barcelo and Stewart, 2002)

Progenitor genotype

Carried in construct

P{UAS-S6k.STDETE}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of S6k (carries the amino acid replacements T398E, S418D and T422E).

(Barcelo and Stewart, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

S6k

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 6

modeled by Pink1^RNAi.UAS.cYa

(Liu and Lu, 2010)

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^UAS.cLa

(Cheng et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size, with Scer\GAL4^AB1

(Georgiev et al., 2010)

increased cell size | somatic clone, with Scer\GAL4^Act.PU

(Mukherjee and Duttaroy, 2013)

short lived, with Scer\GAL4^da.G32

(Kapahi et al., 2004)

visible, with Scer\GAL4^ap-md544

(Lee et al., 2010, Barcelo and Stewart, 2002)

Phenotype Manifest In

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PA

(Bakopoulos et al., 2023)

salivary gland, with Scer\GAL4^AB1

(Georgiev et al., 2010)

wing, with Scer\GAL4^ap-md544

(Barcelo and Stewart, 2002)

wing blade, with Scer\GAL4^ap-md544

(Lee et al., 2010)

Detailed Description

Statement

Reference

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^elav-C155 has no effect on bouton size.

(Deivasigamani et al., 2014)

Expressing S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^da.G32 suppresses the increase in sleep duration seen in wild type flies in response to rapamycin.

(Metaxakis et al., 2014)

Cells in clones in the fat body expressing S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^Act.PU are overgrown.

(Mukherjee and Duttaroy, 2013)

The expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} during the last 48 hours of larval development has no effect on quantal content at the larval neuromuscular junction.

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371 has no effect on bouton size or number at the larval neuromuscular junction.

(Cheng et al., 2011)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^AB1 results in a 135% increase average cell size in the salivary gland.

(Georgiev et al., 2010)

Expression of S6k^{STDETE.Scer\UAS} driven by Scer\GAL4^ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

(Lee et al., 2010)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^elav.PLu has little effect on neuromuscular junction growth.

(Shen and Ganetzky, 2009)

Scer\GAL4^GMR.long>S6k^{STDETE.Scer\UAS} mutants show no defects in opsin regulation.

(Mikeladze-Dvali et al., 2005)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^da.G32 at 29^oC results in a decrease in mean life span of 34% compared to control flies.

(Kapahi et al., 2004)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in wings which bend down (the wings bend down more than in flies expressing S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544).

(Barcelo and Stewart, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^Act.PU has increased cell size | somatic clone phenotype, suppressible | somatic clone by srl^RNAi.UAS.cUa, Scer\GAL4^Act.PU

(Mukherjee and Duttaroy, 2013)

S6k^STDETE.UAS, Scer\GAL4^AB1 has increased cell size phenotype, suppressible by Trpm²⁸

(Georgiev et al., 2010)

NOT suppressed by

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^ap-md544 has visible phenotype, non-suppressible by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

NOT Enhancer of

Statement

Reference

Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS is a non-enhancer of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

Suppressor of

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^pHCl-2.PR is a suppressor of abnormal developmental rate phenotype of Scer\GAL4^pHCl-2.PR, pHCl-2^KK106835

(Redhai et al., 2020)

S6k^STDETE.UAS, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

S6k^STDETE.UAS, Scer\GAL4^salm.EPv is a suppressor of visible phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Scer\GAL4^VGlut1-OK371/S6k^STDETE.UAS is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Pdk1³³

(Cheng et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS is a non-suppressor of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

S6k^STDETE.UAS, Scer\GAL4^109(2)80 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

S6k^STDETE.UAS, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

S6k^STDETE.UAS, Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

S6k^STDETE.UAS, Scer\GAL4^elav.PLu is a non-suppressor of increased cell growth rate phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^AB1 has salivary gland phenotype, suppressible by Trpm²⁸

(Georgiev et al., 2010)

NOT suppressed by

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^ap-md544 has wing blade phenotype, non-suppressible by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

Suppressor of

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^elav-C155 is a suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

S6k^STDETE.UAS, Scer\GAL4^salm.EPv is a suppressor of first posterior wing cell phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

S6k^STDETE.UAS, Scer\GAL4^Act5C.PU is a suppressor | somatic clone of wing disc | somatic clone | third instar larval stage phenotype of Scer\GAL4^Act5C.PU, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

S6k^STDETE.UAS, Scer\GAL4^Act5C.PU is a suppressor | somatic clone of embryonic/larval fat body | somatic clone | third instar larval stage phenotype of Scer\GAL4^Act5C.PU, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Scer\GAL4^VGlut1-OK371/S6k^STDETE.UAS is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Pdk1³³

(Cheng et al., 2011)

NOT Suppressor of

Statement

Reference

S6k^STDETE.UAS, Scer\GAL4^109(2)80 is a non-suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

S6k^STDETE.UAS, Scer\GAL4^109(2)80 is a non-suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Scer\GAL4^bs.PM/S6k^STDETE.UAS is a non-suppressor of terminal tracheal cell | somatic clone | third instar larval stage phenotype of Vha13^cnj-356

(Francis and Ghabrial, 2015)

S6k^STDETE.UAS, Scer\GAL4^elav-C155 is a non-suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

S6k^STDETE.UAS, Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

S6k^STDETE.UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Other

Statement

Reference

Pdk1³³, S6k^STDETE.UAS, Scer\GAL4^VGlut1-OK371 has NMJ bouton | third instar larval stage phenotype

(Cheng et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 is not significantly affected by co-expression of S6k^{STDETE.Scer\UAS}.

(Tsuyama et al., 2017)

Expression of S6k^{STDETE.Scer\UAS} does not suppress the enlarged bouton phenotype seen when Vap-33A^{P58S.cRa.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of S6k^{STDETE.Scer\UAS} suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

The reduction in the distance between wing veins 3 and 4 and the reduction in cell size in the wing that is seen in animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv is suppressed if they are also co-expressing S6k^{STDETE.Scer\UAS}.

Co-expression of S6k^{STDETE.Scer\UAS} rescues the reduced size of clones in third larval instar wing disc and fat body expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU.

(Ibar et al., 2013)

Co-expression of srl^{dsRNA.Scer\UAS.cUa} suppresses the cell overgrowth phenotype seen in cells in clones in the fat body expressing S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^Act.PU.

(Mukherjee and Duttaroy, 2013)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371 partially rescues the reduction in bouton size at the neuromuscular junction which is seen in Pdk1³³ larvae. In addition, the number of boutons at the neuromuscular junction is increased compared to wild type.

(Cheng et al., 2011)

Expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^AB1 in a trpm²⁸ background does not result in an increase in cell size in the larval salivary gland.

(Georgiev et al., 2010)

Co-expression of Sesn^d04539 with S6k^STDETE.UAS via Scer\GAL4^ap-md544 fails to suppress the S6k^STDETE.UAS hyperplastic wing phenotype.

Df(2R)Sesn-8A11 does not enhance the Scer\GAL4^ap-md544-driven S6k^STDETE.UAS-stimulated cell growth in the wing.

(Lee et al., 2010)

Co-expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^elav.PLu, fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1^Scer\UAS.cSa.

(Shen and Ganetzky, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/S6k^STDETE.UAS rescues Df(3L)CH18/S6k^l-1

(Cheng et al., 2011)

Comments

The decrease in bouton size which is seen at the neuromuscular junction in S6k^l-1/Df(3L)CH18 third instar larvae is rescued by expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^elav-C155 and the boutons are actually increased in size compared to wild type in the rescued animals.

The reduced active zone number and reduced reduced total synaptic area seen at the neuromuscular junction of S6k^l-1/Df(3L)CH18 third instar larvae is also rescued by expression of S6k^{STDETE.Scer\UAS} under the control of Scer\GAL4^elav-C155 (the numbers are actually increased in size compared to wild type in the rescued animals).

(Cheng et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6914

w¹¹¹⁸; P{UAS-S6k.STDETE}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

S6K^STDETE

(Mikeladze-Dvali et al., 2005)

S6k^{STDETE.Scer\UAS}

S6k^STDETE.UAS

S6k[STDETE]

(Tsuyama et al., 2017)

dS6K^STDETE

(Georgiev et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (34)

Report Sections

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