FlyBase Allele Report: Dmel\S6k[KQ.UAS]

General Information

Symbol

Dmel\S6k^KQ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0141554

Feature type

allele

Associated gene

Dmel\S6k

Associated Insertion(s)

Carried in Construct

P{UAS-S6k.KQ}

Also Known As

UAS-S6K^KQ, S6K^DN, UAS-S6k.KQ, UAS-S6K^DN, S6K^KQ, UAS-dS6k^KQ, UAS-dS6K^DN, UAS-S6K DN, UAS-S6K KQ

Key Links

Transgenic product class

missense_variant

(Barcelo and Stewart, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Barcelo and Stewart, 2002)

Mutagen

in vitro construct

(Barcelo and Stewart, 2002)

Progenitor genotype

Carried in construct

P{UAS-S6k.KQ}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of S6k (carries the amino acid replacement K109Q).

(Barcelo and Stewart, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

S6k

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

ameliorates congenital muscular dystrophy due to LMNA mutation

modeled by LamC^G489V.UAS

(Chandran et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | oogenesis, with Scer\GAL4^C587

(Su et al., 2018)

abnormal feeding behavior, with Scer\GAL4^Ilp2.PW

(Wu et al., 2005)

abnormal size, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

decreased body size | nutrition conditional, with Scer\GAL4^ppl.PP

(Kamakura, 2011)

decreased cell number, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

decreased cell number | oogenesis | progressive, with Scer\GAL4^C587

(Su et al., 2018)

decreased cell size | somatic clone, with Scer\GAL4^Act.PU

(Mukherjee and Duttaroy, 2013)

long lived, with Scer\GAL4^da.G32

(Kapahi et al., 2004)

long lived, with Scer\GAL4^da.PU

(Lin et al., 2014)

long lived, with Scer\GAL4^Lk6-DJ634

(Kapahi et al., 2004)

long lived, with Scer\GAL4^{Rab27-Gal4-KO}

(Lien et al., 2020)

long lived, with Scer\GAL4^VT014429

(Lien et al., 2020)

visible, with Scer\GAL4^ap-md544

(Mukherjee and Duttaroy, 2013, Lee et al., 2010, Barcelo and Stewart, 2002)

Phenotype Manifest In

escort cell, with Scer\GAL4^C587

(Su et al., 2018)

female germline cyst, with Scer\GAL4^C587

(Su et al., 2018)

female germline stem cell | progressive, with Scer\GAL4^C587

(Su et al., 2018)

wing, with Scer\GAL4^ap-md544

(Mukherjee and Duttaroy, 2013, Barcelo and Stewart, 2002)

wing, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

wing blade, with Scer\GAL4^ap-md544

(Lee et al., 2010)

Detailed Description

Statement

Reference

The adulthood-only expression of S6k^KQ.UAS under the combined control of Scer\GAL4^C587 and Gal80[ts] leads to a progressive decrease in the number of germline stem cells in the germarium, and leads to defective wrapping of germ cysts by escort cells, as compared to controls.

(Su et al., 2018)

Ectopic expression of S6k^KQ.Scer\UAS under the Scer\GAL4^elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

(Wong et al., 2015)

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^elav-C155 has no effect on bouton size.

(Deivasigamani et al., 2014)

Compared with wild-type, expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^da.PU increases mean lifespan.

(Lin et al., 2014)

Cells in clones in the fat body expressing S6k^KQ.Scer\UAS under the control of Scer\GAL4^Act.PU are reduced in size compared to wild-type cells.

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^ap-md544 results in wing bending upwards.

(Mukherjee and Duttaroy, 2013)

Overexpression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^GMR.PF, does not induce autophagy in developing eye discs.

(Shin et al., 2013)

In contrast to wild-type, transgenic flies expressing Scer\GAL4^ppl.PP>S6k^KQ.Scer\UAS and reared on medium supplemented with Apis mellifera royal jelly do not show increased body size. However, similarly to wild-type flies in response to royal jelly, they display shortened developmental time compared with flies reared on control medium.

(Kamakura, 2011)

Expression of S6k^KQ.Scer\UAS using Scer\GAL4^nub-AC-62 results in abnormally small wings in which cell number is decreased.

(Resnik-Docampo and de Celis, 2011)

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of S6k^KQ.Scer\UAS under the simultaneous control of both Scer\GAL4^Scer\UAS.cHa and Scer\GAL4^tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

(Birse et al., 2010)

Expression of S6k^KQ.Scer\UAS driven by Scer\GAL4^ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.

(Lee et al., 2010)

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^elav.PLu has little effect on neuromuscular junction growth.

(Shen and Ganetzky, 2009)

Scer\GAL4^GMR.long>S6k^KQ.Scer\UAS mutants show no defects in opsin regulation.

(Mikeladze-Dvali et al., 2005)

Expression of S6k^KQ.Scer\UAS, under the control of Scer\GAL4^Ilp2 results in a 50% increase in the rate of third instar larval liquid-food intake and a significant feeding on solid food. No significant difference was observed in the size or developmental rate of mutant third instar larvae to wild-type controls. When fed ad libitum, S6k^KQ.Scer\UAS (under the control of Scer\GAL4^NPFR1.6.6) third instar larvae display hyperactive feeding of solid food. However they don't display any increase in the ingestion rate of richer liquid food.

(Wu et al., 2005)

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^da.G32 at 29^oC results in an increase in mean life span of 22% compared to control flies. Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^Lk6-DJ634 at 29^oC results in an increase in mean life span of 29% compared to control flies.

(Kapahi et al., 2004)

Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^ap-md544 results in wings which bend up slightly.

(Barcelo and Stewart, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^nub-AC-62 has abnormal size phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

S6k^KQ.UAS, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

NOT Enhanced by

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^da.PU has long lived phenotype, non-enhanceable by inaE^UAS.cLd, Scer\GAL4^da.PU

(Lin et al., 2014)

Suppressed by

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^Act.PU has decreased cell size | somatic clone phenotype, suppressible | somatic clone by srl^EY05931, Scer\GAL4^Act.PU

(Mukherjee and Duttaroy, 2013)

S6k^KQ.UAS, Scer\GAL4^ap-md544 has visible phenotype, suppressible by srl^EY05931, Scer\GAL4^ap-md544

(Mukherjee and Duttaroy, 2013)

Enhancer of

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, Tak1^UAS.cTa

(Shin et al., 2013)

S6k^KQ.UAS, Scer\GAL4^nub-AC-62 is an enhancer of abnormal size phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

S6k^KQ.UAS, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell size phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ppk.PG/S6k^KQ.UAS is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

S6k^KQ.UAS, Scer\GAL4^da.PU is a non-enhancer of long lived phenotype of Scer\GAL4^da.PU, inaE^UAS.cLd

(Lin et al., 2014)

Suppressor of

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of flightless | progressive phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^NP0001 is a suppressor of increased cell size phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

S6k^KQ.UAS, Scer\GAL4^Tub.PU is a suppressor | partially of lethal - all die during P-stage phenotype of AMPKα^JF01951, Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Scer\GAL4^Tub.PU/S6k^KQ.UAS is a suppressor of lethal - all die during P-stage | recessive phenotype of AMPKα¹

(Onyenwoke et al., 2015)

Scer\GAL4^Tub.PU/S6k^KQ.UAS is a suppressor of lethal - all die during P-stage | recessive phenotype of AMPKα³

(Onyenwoke et al., 2015)

S6k^KQ.UAS, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

S6k^KQ.UAS, Scer\GAL4^ppk.PG is a suppressor | partially of abnormal neuroanatomy | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

S6k^KQ.UAS, Scer\GAL4^rho.PU is a suppressor of increased body size phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^rho.PU

(Kamakura, 2011)

S6k^KQ.UAS, Scer\GAL4^ppl.PP is a suppressor of increased body size phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^ppl.PP

(Kamakura, 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PU/S6k^KQ.UAS is a non-suppressor of abnormal neurophysiology | somatic clone | adult stage phenotype of Nrd1^Q376X

(Yoon et al., 2017)

S6k^KQ.UAS, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

S6k^KQ.UAS, Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

S6k^KQ.UAS, Scer\GAL4^elav.PLu is a non-suppressor of increased cell growth rate phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Other

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^GMR.PF, Tak1^UAS.cTa has lethal - all die before end of pupal stage phenotype

(Shin et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^nub-AC-62 has wing phenotype, enhanceable by hppy^UAS.cMa, Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

S6k^KQ.UAS, Scer\GAL4^ap-md544 has wing blade phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

Suppressed by

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^ap-md544 has wing phenotype, suppressible by srl^EY05931, Scer\GAL4^ap-md544

(Mukherjee and Duttaroy, 2013)

Enhancer of

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ppk.PG/S6k^KQ.UAS is a non-enhancer of larval multidendritic class IV neuron phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Suppressor of

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of indirect flight muscle cell | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of wing phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of nucleus | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of myofibril | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a suppressor of mitochondrion | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

S6k^KQ.UAS, Scer\GAL4^NP0001 is a suppressor of adult midgut epithelium phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

S6k^KQ.UAS, Scer\GAL4^elav-C155 is a suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

S6k^KQ.UAS, Scer\GAL4^ppk.PG is a suppressor | partially of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

NOT Suppressor of

Statement

Reference

S6k^KQ.UAS, Scer\GAL4^fln.IFM is a non-suppressor of indirect flight muscle cell | adult stage phenotype of LamC^R205W.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Scer\GAL4^ninaE.PU/S6k^KQ.UAS is a non-suppressor of retina | somatic clone | adult stage phenotype of Nrd1^Q376X

(Yoon et al., 2017)

S6k^KQ.UAS, Scer\GAL4^elav-C155 is a non-suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

S6k^KQ.UAS, Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

S6k^KQ.UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of LamC^UAS.cSa and S6k^KQ.UAS under the control of Scer\GAL4^fln.IFM under the control of Scer\GAL4^fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

(Chandran et al., 2019)

The co-expression of S6k^KQ.Scer\UAS suppresses the increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

The loss of synaptic transmission in photoreceptor cells (characterized by loss of on- and off-transients in electroretinogram readings) observed in aged adult flies with Nrd1^Q376X mutant retinal somatic clones is partially suppressed by expression of S6k^KQ.Scer\UAS controlled by the Scer\GAL4^ninaE.PU driver.

(Yoon et al., 2017)

The late pupal lethality observed in animals expressing AMPKα^JF01951 under the control of Scer\GAL4^tub.PU can be partially rescued by co-expression of S6k^KQ.Scer\UAS.

The pupal-stage lethality of either hemizygous AMPKα¹ or AMPKα³ males can be partially rescued by expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^tub.PU.

(Onyenwoke et al., 2015)

Expression of S6k^KQ.Scer\UAS suppresses the enlarged bouton phenotype seen when Vap-33A^{P58S.cRa.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of S6k^KQ.Scer\UAS does not suppress the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

Simultaneous expression of inaE^Scer\UAS.cLd and S6k^KQ.Scer\UAS both under the control of Scer\GAL4^da.PU does not further extend lifespan compared with the expression of either transgene alone.

(Lin et al., 2014)

Co-expression of srl^EY05931 suppresses the reduced cell size seen in cells in clones in the fat body expressing S6k^KQ.Scer\UAS under the control of Scer\GAL4^Act.PU.

Co-expression of srl^EY05931 almost completely rescues the bent upwards wing phenotype caused by expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^ap-md544.

(Mukherjee and Duttaroy, 2013)

The number of autolysosomes, indicating the level of autophagy, is increased upon co-expression of S6k^KQ.Scer\UAS and Tak1^Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4^GMR.PF, compared to expression of either transgene alone.

(Shin et al., 2013)

Expression of S6k^KQ.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19^GD9650 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

(Wong et al., 2013)

Co-expression of S6k^KQ.Scer\UAS and hppy^Scer\UAS.cMa using Scer\GAL4^nub-AC-62 results in a moderately synergistic phenotype of reduced wing size.

(Resnik-Docampo and de Celis, 2011)

Co-expression of Sesn^d04539 with S6k^KQ.UAS driven by Scer\GAL4^ap-md544 enhances the wing-bending phenotype of S6k^KQ.UAS.

Df(2R)Sesn-8A11 does not enhance the growth suppression resulting from Scer\GAL4^ap-md544-driven S6k^KQ.UAS expression in the wing.

(Lee et al., 2010)

Co-expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^elav.PLu, fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1^Scer\UAS.cSa.

(Shen and Ganetzky, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^elav-C155/S6k^KQ.UAS fails to rescue Df(3L)CH18/S6k^l-1

(Cheng et al., 2011)

Comments

The decrease in bouton size which is seen at the neuromuscular junction in S6k^l-1/Df(3L)CH18 third instar larvae is not rescued by expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^elav-C155.

(Cheng et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6911

w¹¹¹⁸; P{UAS-S6k.KQ}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

S6K^DN

(Birse et al., 2010)

S6K^KQ

(Mikeladze-Dvali et al., 2005)

S6k^DN

(Lee et al., 2021)

S6k^KQ.Scer\UAS

S6k^KQ.UAS

Name Synonyms

Secondary FlyBase IDs

References (35)

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