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General Information
Symbol
Dmel\S6kKQ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141554
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-S6KKQ, S6KKQ, S6KDN, UAS-dS6kKQ, UAS-S6k.KQ, UAS-S6KDN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated form of S6k (carries the amino acid replacement K109Q).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The adulthood-only expression of S6kKQ.UAS under the combined control of Scer\GAL4C587 and Gal80[ts] leads to a progressive decrease in the number of germline stem cells in the germarium, and leads to defective wrapping of germ cysts by escort cells, as compared to controls.

Ectopic expression of S6kKQ.Scer\UAS under the Scer\GAL4elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4elav-C155 has no effect on bouton size.

Compared with wild-type, expression of S6kKQ.Scer\UAS under the control of Scer\GAL4da.PU increases mean lifespan.

Cells in clones in the fat body expressing S6kKQ.Scer\UAS under the control of Scer\GAL4Act.PU are reduced in size compared to wild-type cells.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4ap-md544 results in wing bending upwards.

Overexpression of S6kKQ.Scer\UAS under the control of Scer\GAL4GMR.PF, does not induce autophagy in developing eye discs.

In contrast to wild-type, transgenic flies expressing Scer\GAL4ppl.PP>S6kKQ.Scer\UAS and reared on medium supplemented with Apis mellifera royal jelly do not show increased body size. However, similarly to wild-type flies in response to royal jelly, they display shortened developmental time compared with flies reared on control medium.

Expression of S6kKQ.Scer\UAS using Scer\GAL4nub-AC-62 results in abnormally small wings in which cell number is decreased.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of S6kKQ.Scer\UAS under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

Expression of S6kKQ.Scer\UAS driven by Scer\GAL4ap-md544 in dorsal cells of the wing causes a phenotype in which the wing bends upward.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4elav.PLu has little effect on neuromuscular junction growth.

Scer\GAL4GMR.long>S6kKQ.Scer\UAS mutants show no defects in opsin regulation.

Expression of S6kKQ.Scer\UAS, under the control of Scer\GAL4Ilp2 results in a 50% increase in the rate of third instar larval liquid-food intake and a significant feeding on solid food. No significant difference was observed in the size or developmental rate of mutant third instar larvae to wild-type controls. When fed ad libitum, S6kKQ.Scer\UAS (under the control of Scer\GAL4NPFR1.6.6) third instar larvae display hyperactive feeding of solid food. However they don't display any increase in the ingestion rate of richer liquid food.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4da.G32 at 29oC results in an increase in mean life span of 22% compared to control flies. Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4Lk6-DJ634 at 29oC results in an increase in mean life span of 29% compared to control flies.

Expression of S6kKQ.Scer\UAS under the control of Scer\GAL4ap-md544 results in wings which bend up slightly.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

S6kKQ.UAS, Scer\GAL4ap-md544 has wing blade phenotype, non-enhanceable by Sesn8A11

Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of LamCUAS.cSa and S6kKQ.UAS under the control of Scer\GAL4fln.IFM under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

The co-expression of S6kKQ.Scer\UAS suppresses the increased midgut cell size resulting from the expression of Atg9HMS01246 under the control of Scer\GAL4Myo31DF-NP0001.

The loss of synaptic transmission in photoreceptor cells (characterized by loss of on- and off-transients in electroretinogram readings) observed in aged adult flies with Nrd1Q376X mutant retinal somatic clones is partially suppressed by expression of S6kKQ.Scer\UAS controlled by the Scer\GAL4ninaE.PU driver.

The late pupal lethality observed in animals expressing AMPKαJF01951 under the control of Scer\GAL4tub.PU can be partially rescued by co-expression of S6kKQ.Scer\UAS.

The pupal-stage lethality of either hemizygous AMPKα1 or AMPKα3 males can be partially rescued by expression of S6kKQ.Scer\UAS under the control of Scer\GAL4tub.PU.

Expression of S6kKQ.Scer\UAS suppresses the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of S6kKQ.Scer\UAS does not suppress the small bouton phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4elav-C155.

Simultaneous expression of inaEScer\UAS.cLd and S6kKQ.Scer\UAS both under the control of Scer\GAL4da.PU does not further extend lifespan compared with the expression of either transgene alone.

Co-expression of srlEY05931 suppresses the reduced cell size seen in cells in clones in the fat body expressing S6kKQ.Scer\UAS under the control of Scer\GAL4Act.PU.

Co-expression of srlEY05931 almost completely rescues the bent upwards wing phenotype caused by expression of S6kKQ.Scer\UAS under the control of Scer\GAL4ap-md544.

The number of autolysosomes, indicating the level of autophagy, is increased upon co-expression of S6kKQ.Scer\UAS and Tak1Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4GMR.PF, compared to expression of either transgene alone.

Expression of S6kKQ.Scer\UAS dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Co-expression of S6kKQ.Scer\UAS and hppyScer\UAS.cMa using Scer\GAL4nub-AC-62 results in a moderately synergistic phenotype of reduced wing size.

Co-expression of Sesnd04539 with S6kKQ.Scer\UAS driven by Scer\GAL4ap-md544 enhances the wing-bending phenotype of S6kKQ.Scer\UAS.

Sesn8A11 does not enhance the growth suppression resulting from Scer\GAL4ap-md544-driven S6kKQ.Scer\UAS expression in the wing.

Co-expression of S6kKQ.Scer\UAS under the control of Scer\GAL4elav.PLu, fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1Scer\UAS.cSa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The decrease in bouton size which is seen at the neuromuscular junction in S6kl-1/Df(3L)CH18 third instar larvae is not rescued by expression of S6kKQ.Scer\UAS under the control of Scer\GAL4elav-C155.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
S6kKQ.Scer\UAS
S6kKQ.UAS
Name Synonyms
Secondary FlyBase IDs
    References (23)