Allele Dmel\S6k^KQ.Scer\UAS

General Information
Symbol	Dmel\S6k^KQ.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0141554
Feature type	allele	Associated gene	Dmel\S6k

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Barcelo and Stewart, 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: K109Q. Scer\UAS regulatory sequences drive expression of a mutated form of S6k. (Barcelo and Stewart, 2002)
Carried in construct	P{UAS-S6k.KQ} (Wu et al., 2005, Barcelo and Stewart, 2002, Kapahi et al., 2004, Mikeladze-Dvali et al., 2005, Cheng et al., 2011, Lee et al., 2010, Resnik-Docampo and de Celis, 2011, Shen and Ganetzky, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) feeding behavior defective, with Scer\GAL4^Ilp2.PW (Wu et al., 2005) long lived, with Scer\GAL4^da.G32 (Kapahi et al., 2004) long lived, with Scer\GAL4^Lk6-DJ634 (Kapahi et al., 2004) size defective, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) visible, with Scer\GAL4^ap-md544 (Barcelo and Stewart, 2002, Lee et al., 2010)
Phenotype Manifest In
	wing, with Scer\GAL4^ap-md544 (Barcelo and Stewart, 2002) wing, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) wing blade, with Scer\GAL4^ap-md544 (Lee et al., 2010)
Detailed Description
	Statement Reference Expression of S6k[KQ.Scer\UAS] using Scer\GAL4[nub-AC-62] results in abnormally small wings in which cell number is decreased. (Resnik-Docampo and de Celis, 2011) Expression of S6k[KQ.Scer\UAS] under the control of Scer\GAL4[Lsp2.PH] rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet. Expression of S6k[KQ.Scer\UAS] under the simultaneous control of both Scer\GAL4[Scer\UAS.cHa] and Scer\GAL4[tin.cBa] does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals. (Birse et al., 2010) Expression of S6k[KQ.Scer\UAS] driven by Scer\GAL4[ap-md544] in dorsal cells of the wing causes a phenotype in which the wing bends upward. (Lee et al., 2010) Expression of S6k[KQ.Scer\UAS] under the control of Scer\GAL4[elav.PLu] has little effect on neuromuscular junction growth. (Shen and Ganetzky, 2009) Scer\GAL4^GMR.long>S6k^KQ.Scer\UAS mutants show no defects in opsin regulation. (Mikeladze-Dvali et al., 2005) Expression of S6k^KQ.Scer\UAS, under the control of Scer\GAL4^Ilp2 results in a 50% increase in the rate of third instar larval liquid-food intake and a significant feeding on solid food. No significant difference was observed in the size or developmental rate of mutant third instar larvae to wild-type controls. When fed ad libitum, S6k^KQ.Scer\UAS (under the control of Scer\GAL4^NPFR1.6.6) third instar larvae display hyperactive feeding of solid food. However they don't display any increase in the ingestion rate of richer liquid food. (Wu et al., 2005) Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^da.G32 at 29^oC results in an increase in mean life span of 22% compared to control flies. Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^Lk6-DJ634 at 29^oC results in an increase in mean life span of 29% compared to control flies. (Kapahi et al., 2004) Expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^ap-md544 results in wings which bend up slightly. (Barcelo and Stewart, 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544 (Lee et al., 2010) S6k^KQ.Scer\UAS, Scer\GAL4^nub-AC-62 has size defective phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
NOT Enhanced by
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^ap-md544 has visible phenotype, non-enhanceable by Sesn^8A11 (Lee et al., 2010)
Enhancer of
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell size phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011) S6k^KQ.Scer\UAS, Scer\GAL4^nub-AC-62 is an enhancer of size defective phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011)
NOT Suppressor of
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of increased cell growth phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) S6k^KQ.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuroanatomy defective phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009)
Phenotype Manifest In
Enhanced by
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^ap-md544 has wing blade phenotype, enhanceable by Sesn^d04539, Scer\GAL4^ap-md544 (Lee et al., 2010) S6k^KQ.Scer\UAS, Scer\GAL4^nub-AC-62 has wing phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
NOT Enhanced by
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^ap-md544 has wing blade phenotype, non-enhanceable by Sesn^8A11 (Lee et al., 2010)
Enhancer of
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011)
NOT Suppressor of
Statement Reference S6k^KQ.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of neuromuscular junction phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009) S6k^KQ.Scer\UAS, Scer\GAL4^elav.PLu is a non-suppressor of NMJ bouton phenotype of Atg1^Scer\UAS.cSa, Scer\GAL4^elav.PLu (Shen and Ganetzky, 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of S6k[KQ.Scer\UAS] and hppy[Scer\UAS.cMa] using Scer\GAL4[nub-AC-62] results in a moderately synergistic phenotype of reduced wing size. (Resnik-Docampo and de Celis, 2011) Co-expression of Sesn[d04539] with S6k[KQ.Scer\UAS] driven by Scer\GAL4[ap-md544] enhances the wing-bending phenotype of S6k[KQ.Scer\UAS]. Sesn[8A11] does not enhance the growth suppression resulting from Scer\GAL4[ap-md544]-driven S6k[KQ.Scer\UAS] expression in the wing. (Lee et al., 2010) Co-expression of S6k[KQ.Scer\UAS] under the control of Scer\GAL4[elav.PLu], fails to suppress the neuromuscular junction overgrowth phenotype found upon expression of Atg1[Scer\UAS.cSa]. (Shen and Ganetzky, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	Scer\GAL4^elav-C155/S6k^KQ.Scer\UAS fails to rescue Df(3L)CH18/S6k^l-1 (Cheng et al., 2011)
Comments	The decrease in bouton size which is seen at the neuromuscular junction in S6k[l-1]/Df(3L)CH18 third instar larvae is not rescued by expression of S6k[KQ.Scer\UAS] under the control of Scer\GAL4[elav-C155]. (Cheng et al., 2011)
Stocks ( 1 )
Bloomington	6911 w¹¹¹⁸; P{UAS-S6k.KQ}2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	S6K^DN (Birse et al., 2010) S6k^KQ.Scer\UAS S6K^KQ (Mikeladze-Dvali et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Cheng et al., 2011, J. Cell Biol. 194(6): 921--935 S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development. [FBrf0216911] Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911] Birse et al., 2010, Cell Metab. 12(5): 533--544 High-fat-diet-induced obesity and heart dysfunction are regulated by the TOR pathway in Drosophila. [FBrf0212220] Lee et al., 2010, Science 327(5970): 1223--1228 Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. [FBrf0210161] Shen and Ganetzky, 2009, J. Cell Biol. 187(1): 71--79 Autophagy promotes synapse development in Drosophila. [FBrf0208921] Mikeladze-Dvali et al., 2005, Cell 122(5): 775--787 The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors. [FBrf0187233] Wu et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(37): 13289--13294 Regulation of hunger-driven behaviors by neural ribosomal S6 kinase in Drosophila. [FBrf0188283] Kapahi et al., 2004, Curr. Biol. 14(10): 885--890 Regulation of lifespan in Drosophila by modulation of genes in the TOR signaling pathway. [FBrf0179269] Barcelo and Stewart, 2002, genesis 34(1-2): 83--85 Altering Drosophila S6 kinase activity is consistent with a role for S6 kinase in growth. [FBrf0152384]

Allele Dmel\S6kKQ.Scer\UAS

Allele Dmel\S6k^KQ.Scer\UAS