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General Information
Symbol
Dmel\Prosβ21.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141583
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DTS7, UAS-Prosβ21
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Prosβ21.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.long leads to rough eye phenotype due to ommatidial degeneration in adult flies maintained at 28[o]C, the phenotype is more severe when two copies of the transgene are used.

Expression of temperature-sensitive Prosβ2 under the control of Scer\GAL4GMR.PF results in rough eye phenotype at the non-permissive temperature of 28[o]C, at the permissive temperature (22[o]C) the eyes appear normal.

Co-expression of Prosβ21.Scer\UAS and Prosβ61.B.Scer\UAS under the control of Scer\GAL4wor.PA does not induce larval neuroblast loss.

Expression of Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF has no effect on eye pigmentation.

Flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF have normal eye morphology at 22oC, but at 28oC, substantial retinal degeneration is seen, resulting in a rough eye phenotype.

Photoreceptor neurons show a significant increase in morphological features of autophagy in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC; there is an increase in the number of autophagic vacuoles and in the number of multilamellar bodies/multivesicular bodies.

Expression of Prosβ21.Scer\UAS at 29oC under the control of Scer\GAL4GMR.PF results in severely disrupted eyes, with fused facets and small spots of black, presumably necrotic, cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Flies co-expressing Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4elav.PU at the non-permissive temperature 30[o]C are lethal at the third instar larval stage. When the flies are raised at 18[o]C and then incubated at 30[o]C for 4 weeks and exposed to the pesticides paraquat and/or maneb no dopaminergic neuron loss is observed.

Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS in the salivary gland under the control of Scer\GAL4fkh.PH causes aberrant morphology of some salivary gland nuclei.

Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PU at 29[o]C results in a rough eye phenotype. This phenotype is enhanced if the animals are also co-expressing either TnksGD11407 or CG9588HM05013.

Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PU at 29[o]C results in a rough eye phenotype. This phenotype is enhanced in females if they are also co-expressing CG9588GD14259.

Co-expression of Prosβ61.B.Scer\UAS, Prosβ21.Scer\UAS and TnksKK100530 under the control of Scer\GAL4GMR.PU at 29[o]C results in lethality.

Co-expression of Prosβ61.B.Scer\UAS, Prosβ21.Scer\UAS and CG9588GD14259 under the control of Scer\GAL4GMR.PU at 29[o]C results in male lethality.

Expression of both Pros261.B.Scer\UAS and Prosβ21.Scer\UAS using Scer\GAL4GMR.PU at 29[o]C causes a rough eye phenotype.

Co-expression of PI31Scer\UAS.T:Ivir\HA1 suppresses the rough eye seen in flies expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS using Scer\GAL4GMR.PU at 29[o]C.

Whereas Scer\GAL4GMR.PU-mediated expression of either Prosβ21.Scer\UAS or PI31GD13764 alone gives no detectable eye phenotype at 25[o]C, their co-expression causes a strong eye phenotype. At 29[o]C, this combination is lethal.

Flies co-expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 25[o]C appear near normal. Additional co-expression of P{Sym-UAS-Hsrω} does not alter the external eye morphology. However, the pseudopupil images of flies expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS reveal severe retinal damage, which is substantially rescued by additional co-expression of one copy of P{Sym-UAS-Hsrω}.

Expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS in the developing tracheal system under the control of Scer\GAL4btl.PS is sufficient to cause dorsal trunk breaks due to down-regulation of trh in embryonic fusion cells.

The retinal degeneration seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of HDAC6Scer\UAS.T:SV5\V5.

The rough eye phenotype seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is enhanced by co-expression of Atg6dsRNA.Scer\UAS or Atg12dsRNA.Scer\UAS.

The ability of HDAC6Scer\UAS.T:SV5\V5 to suppress the rough eye phenotype seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of Atg12dsRNA.Scer\UAS.

Xenogenetic Interactions
Statement
Reference

The eye degeneration phenotype observed in adult flies expressing either Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP or Zzzz\CGG100.5'UTR.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.long (and maintained at 28[o]C) is significantly enhanced by co-expression of Prosβ21.Scer\UAS (which on its own also induces rough eye phenotype), the eye degeneration is most severe when two copies of the Prosβ21.Scer\UAS transgene are used. Similarly, co-expression of Prosβ21.Scer\UAS also enhances the formation of inclusions in the ommatidia of Zzzz\CGG100.5'UTR.Scer\UAS.T:Avic\GFP-expressing flies.

Co-expression of Prosβ21.Scer\UAS with either Zzzz\CGG100.5'Stop.Scer\UAS.T:Avic\GFP or Zzzz\CGG100.5'ATG.Scer\UAS.T:Avic\GFP also leads to mutual enhancement of the rough eye phenotype (of varied severity) each of the transgene produces on its own - combination with Zzzz\CGG100.5'Stop.Scer\UAS.T:Avic\GFP produces rather weak while combination with Zzzz\CGG100.5'ATG.Scer\UAS.T:Avic\GFP very strong phenotype enhancement (all ommatidia fused along with necrotic lesions).

Co-expression of the temperature sensitive Prosβ21.Scer\UAS allele together with Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 has no adverse effect on the morphology and structure of the adult thorax or the thoracic somatic muscles below at either the non-permissive (28[o]C) or the permissive (22[o]C) temperatures.

Simultaneous co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Simultaneous co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS leads to lethality in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies; extra co-expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 suppresses this lethality.

Expression of Prosβ21.Scer\UAS enhances the eye pigmentation phenotype seen when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed under the control of Scer\GAL4GMR.PF. This enhancement is seen throughout adult life at 25[o]C.

Expression of Prosβ21.Scer\UAS enhances the eye pigmentation phenotype seen when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed under the control of Scer\GAL4GMR.PF. This enhancement is seen throughout adult life at 25[o]C.

The eye damage caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is enhanced by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eyes.

The increase in inclusion bodies in larval eye discs caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is further increased by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. However, co-expression of P{Sym-UAS-Hsrω} fails to reduce the number of inclusion bodies in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS background.

The damaged eye phenotype that results from Scer\GAL4GMR.PF-mediated expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 is not further enhanced by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 eyes.

The retinal degeneration seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of Hsap\HDAC6Scer\UAS.cPa, but not by co-expression of Hsap\HDAC6H216A.H611A.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Prosβ21.Scer\UAS
Prosβ21.UAS
Name Synonyms
Secondary FlyBase IDs
    References (25)