Allele Dmel\N^{dsRNA.P.Scer\UAS}

General Information
Symbol	Dmel\N^{dsRNA.P.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0141643
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\N

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| RNAi
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Presente et al., 2002) in vitro construct \| RNAi (Presente et al., 2002)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of N sequences arranged in an inverted repeat (each copy of the repeat is a 1001bp fragment from N exon 6). (Presente et al., 2002)
Assay mode	In transgenic Drosophila (intraspecific) (Presente et al., 2002)
Carried in construct	P{UAS-N.dsRNA.P} (Micchelli and Perrimon, 2006, Presente et al., 2002, Presente et al., 2004, Assa-Kunik et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^hs.2sev (Presente et al., 2002) visible, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) visible, with Scer\GAL4^GMR.PF (Presente et al., 2002) visible, with Scer\GAL4^bbg-C96 (Presente et al., 2002) memory defective, with Scer\GAL4^c747 (Presente et al., 2004) chemosensitive behavior defective, with Scer\GAL4^A307 (Presente et al., 2002)
Phenotype Manifest In
	adult midgut \| conditional ts, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Micchelli and Perrimon, 2006) wing margin, with Scer\GAL4^bbg-C96 (Presente et al., 2002) wing, with Scer\GAL4^bbg-C96 (Presente et al., 2002) eye, with Scer\GAL4^hs.2sev (Presente et al., 2002) ommatidium, with Scer\GAL4^hs.2sev (Presente et al., 2002) eye, with Scer\GAL4^GMR.PF (Presente et al., 2002) ommatidium, with Scer\GAL4^GMR.PF (Presente et al., 2002) adult thorax, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) macrochaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Presente et al., 2002) macrochaeta, with Scer\GAL4^pnr-MD237 (Presente et al., 2002)
Detailed Description
	Statement Reference Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^C96 results in a number of wing phenotypes depending on the P{UAS-N.dsRNA.P} insertion line used, ranging from no defects in wing margin formation, through subtle bristle defects, a consistent notched phenotype to extreme loss of dorsal and ventral wing tissue. Expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF results in a rough eye phenotype. Animals expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 show balding of the thorax and either loss of notal macrochaetae or multiplication of macrochaetae (depending on the P{UAS-N.dsRNA.P} insertion line used). Animals expressing N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^A307 have a markedly impaired startle response to proprionic acid vapour compared to controls. (Presente et al., 2002) When N^{dsRNA.P.Scer\UAS} is driven by Scer\GAL4^c747, short term memory is normal, however long term memory is impaired. (Presente et al., 2004) Adults carrying N^{dsRNA.P.Scer\UAS}, Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B and raised at the permissive temperature have wild-type midguts (at this temperature, the Scer\GAL80^ts.αTub84B protein inhibits Scer\GAL4^esg-NP5130). When these flies are shifted to the non-permissive temperature (which inactivates Scer\GAL80^ts.αTub84B, allowing expression of N^{dsRNA.P.Scer\UAS} under the control of Scer\GAL4^esg-NP5130), there is an increase in the number of small cells in the adult midgut epithelium. (Micchelli and Perrimon, 2006)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	7077 w^; P{UAS-N.dsRNA.P}9G 7076 w^; P{UAS-N.dsRNA.P}14A 7078 P{UAS-N.dsRNA.P}14E, w^*
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	N^{dsRNA.P.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Assa-Kunik et al., 2007, Development 134(6): 1161--1169 Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways. [FBrf0195377] Micchelli and Perrimon, 2006, Nature 439(7075): 475--479 Evidence that stem cells reside in the adult Drosophila midgut epithelium. [FBrf0191249] Presente et al., 2004, Proc. Natl. Acad. Sci. USA 101(6): 1764--1768 Notch is required for long-term memory in Drosophila. [FBrf0175078] Presente et al., 2002, genesis 34(1-2): 165--169 Transgene-mediated RNA interference defines a novel role for Notch in chemosensory startle behavior. [FBrf0152371]

Allele Dmel\NdsRNA.P.Scer\UAS

Allele Dmel\N^{dsRNA.P.Scer\UAS}