Allele Dmel\Mekk1^Ur36

General Information
Symbol	Dmel\Mekk1^Ur36	Species	D. melanogaster
Name		FlyBase ID	FBal0141666
Feature type	allele	Associated gene	Dmel\Mekk1
Also Known As	dMekk1^Ur36

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Inoue et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Mekk1^S028102a (Inoue et al., 2001)
Nature of the lesion	Statement Reference Deletion of the P{lacW} of the progenitor allele and 868 bp of flanking DNA, removing amino acids 1194-1483. The deleted section includes the kinase subdomain I-IX. (Inoue et al., 2001)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant (Kang et al., 2012) chemical sensitive (Brun et al., 2006) enhancer of variegation (Seong et al., 2011) heat stress response defective (Inoue et al., 2001, Seong et al., 2011) lethal \| conditional (Inoue et al., 2001) melanotic mass phenotype \| larval stage (Seisenbacher et al., 2011) melanotic mass phenotype \| larval stage \| nutrition conditional (Seisenbacher et al., 2011) osmotic stress response defective (Inoue et al., 2001) stress response defective (Brun et al., 2006) stress response defective \| recessive (Craig et al., 2004) viable (Inoue et al., 2001)
Phenotype Manifest In
	embryonic/larval hindgut \| larval stage \| posterior (Seisenbacher et al., 2011) embryonic/larval hindgut \| larval stage \| posterior \| nutrition conditional (Seisenbacher et al., 2011)
Detailed Description
	Statement Reference Mutant flies are more resistant to tunicamycin compared to controls. (Kang et al., 2012) Mekk1[Ur36] mutant larvae exhibit a melanotic phenotype under normal food conditions. A "black dot" is seen in the posterior hindgut. The phenotype is exacerbated in response to salt stress. (Seisenbacher et al., 2011) Mekk1[Ur36] heterozygotes show enhanced w silencing in the In(1)w[m4] line. In contrast to controls, Mekk1[Ur36] mutant flies do not show relieved w silencing in the In(1)w[m4] line when embryos are exposed to heat-shock 0-3 hours after egg laying. (Seong et al., 2011) Mutant flies show normal levels of survival after infection after infection with E.coli, E.faecalis or A.fumigatus. Homozygous flies show a strong increase in sensitivity to oxidative stress induced by paraquat compared to controls. (Brun et al., 2006) Shows increased susceptibility to high osmolarity (0.2M NaCl). Effect less pronounced than reported in FBrf0139644. No immune reponse deficiency could be detected. Response to bacterial infection was not compromised. Wound healing appears normal. (Craig et al., 2004) Homozygotes and hemizygotes show no obvious morphological abnormalities. Response to heat stress and osmotic stress is defective. Viability of mutants relative to wild type is reduced by 20% at 30^oC. Mutants die before eclosion when raised on 0.2M NaCl. (Inoue et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Mekk1^Ur36/Mekk1[+] is an enhancer of visible phenotype of Rala^35d (Balakireva et al., 2006)
Suppressor of
Statement Reference Mekk1^Ur36/Mekk1^Ur36 is a suppressor \| partially of visible phenotype of Scer\GAL4^GMR.PF, ninaE^{G69D.Scer\UAS} (Kang et al., 2012, Kang et al., 2012) Mekk1^Ur36/Mekk1^Ur36 is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, ninaE^{G69D.Scer\UAS} (Kang et al., 2012, Kang et al., 2012)
Phenotype Manifest In
Enhancer of
Statement Reference Mekk1^Ur36/Mekk1[+] is an enhancer of macrochaeta phenotype of Rala^35d (Balakireva et al., 2006) Mekk1^Ur36/Mekk1[+] is an enhancer of microchaeta phenotype of Rala^35d (Balakireva et al., 2006)
Suppressor of
Statement Reference Mekk1^Ur36/Mekk1^Ur36 is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PF, ninaE^{G69D.Scer\UAS} (Kang et al., 2012, Kang et al., 2012) Mekk1^Ur36/Mekk1^Ur36 is a suppressor \| partially of retina \| conditional phenotype of ninaE^G69D (Kang et al., 2012, Kang et al., 2012) Mekk1^Ur36/Mekk1^Ur36 is a suppressor of eye disc phenotype of Scer\GAL4^GMR.PF, ninaE^{G69D.Scer\UAS} (Kang et al., 2012, Kang et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Mekk1[Ur36]/Mekk1[Ur36] partially suppresses the eye defects caused by expression of ninaE[G69D.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. The massive apoptosis seen in the larval eye discs of animals expressing ninaE[G69D.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is suppressed by Mekk1[Ur36]/Mekk1[Ur36]. The time course of retinal degeneration seen in ninaE[G69D]/+ flies is delayed in a Mekk1[Ur36]/Mekk1[Ur36] background. (Kang et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Mekk1^Ur36 is rescued by Mekk1^hs.PI (Inoue et al., 2001)
Partially rescued by	Mekk1^Ur36 is partially rescued by Mekk1^hs.PI (Brun et al., 2006)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	dMEKK1^Ur3 (Chakrabarti et al., 2012) D-MEKK1^Ur36 (Craig et al., 2004) dMekk1^Ur36 (Shimizu et al., 2008, Seong et al., 2011) dMekk1^ur36 (Seong et al., 2011) Mekk1^Ur36 (Brun et al., 2006) mekk1^ur-36 (Kang et al., 2012) MEKK1^Ur36 (Ha et al., 2009, Seisenbacher et al., 2011) mekk1^Ur36 (Balakireva et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Kang et al., 2012, Nat. Cell Biol. 14(4): 409--415 CDK5 and MEKK1 mediate pro-apoptotic signalling following endoplasmic reticulum stress in an autosomal dominant retinitis pigmentosa model. [FBrf0217924] Seisenbacher et al., 2011, PLoS Genet. 7(8): e1002168 MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic Stress. [FBrf0214607] Seong et al., 2011, Cell 145(7): 1049--1061 Inheritance of Stress-Induced, ATF-2-Dependent Epigenetic Change. [FBrf0214019] Ha et al., 2009, Nat. Immunol. 10(9): 949--957 Coordination of multiple dual oxidase-regulatory pathways in responses to commensal and infectious microbes in drosophila gut. [FBrf0208708] Shimizu et al., 2008, Mol. Cell. Biol. 28(20): 6278--6289 Drosophila ATF-2 regulates sleep and locomotor activity in pacemaker neurons. [FBrf0207384] Balakireva et al., 2006, Mol. Cell. Biol. 26(23): 8953--8963 The Ral/exocyst effector complex counters c-Jun N-terminal kinase-dependent apoptosis in Drosophila melanogaster. [FBrf0194568] Brun et al., 2006, Genes Cells 11(4): 397--407 The MAPKKK Mekk1 regulates the expression of Turandot stress genes in response to septic injury in Drosophila. [FBrf0190571] Craig et al., 2004, EMBO Rep. 5(11): 1058--1063 A Drosophila p38 orthologue is required for environmental stress responses. [FBrf0180205] Inoue et al., 2001, EMBO J. 20(19): 5421--5430 A Drosophila MAPKKK, D-MEKK1, mediates stress responses through activation of p38 MAPK. [FBrf0139644]
Supplementary material	Ha et al., 2009, Nat. Immunol. 10(9): Online methods. [FBrf0209482]

Allele Dmel\Mekk1Ur36

Allele Dmel\Mekk1^Ur36