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General Information
Symbol
Dmel\CkIIαTik
Species
D. melanogaster
Name
Timekeeper
FlyBase ID
FBal0141857
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
CK2αTik, Tik
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
T23102733A
Amino acid change:
M161K | CkIIalpha-PA; M161K | CkIIalpha-PB; M161K | CkIIalpha-PC; M161K | CkIIalpha-PD; M161K | CkIIalpha-PE; M161K | CkIIalpha-PF; M161K | CkIIalpha-PG
Reported amino acid change:
M161K
Comment:
One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
point mutation
Nucleotide change:
A23102746Y
Amino acid change:
E165D | CkIIalpha-PA; E165D | CkIIalpha-PB; E165D | CkIIalpha-PC; E165D | CkIIalpha-PD; E165D | CkIIalpha-PE; E165D | CkIIalpha-PF; E165D | CkIIalpha-PG
Reported amino acid change:
E165D
Comment:
One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: E165?.
Amino acid replacement: M161K.
CkIIαTik contains two coding mutations within the presumptive ATP binding site.
Amino acid replacement: M161K. Amino acid replacement: E165D.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
CkIIαP1/CkIIαTik transheterozygotes exhibit synaptic retraction in ~40% of neuromuscular junctions in muscles 1/9 and 2/10.
Approximately 20% of eggs laid by CkIIαTik/+ females have ventralised chorions.
The wings of CkIIαTik/+ flies appear wild type.
CkIIαTik/Y adults have a longer circadian periodicity of locomotor activity than wild-type controls.
Expression of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel in a CkIIαTik/+ background results in a more severe eye phenotype than expression in a wild-type background. Scer\GAL4ey.3.5.Exel>CkIIαdsRNA.Scer\UAS, CkIIαTik/+ eyes are rough in all sections, slightly reduced in size, display fused ommatidia, and have an interommatidial bristle patterning that is more defective. CkIIαTik/+ flies do not display bristle defects. Expression of one copy of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4sca-537.4 in a CkIIαTik/+ background results in split bristles and branching of the shaft cell.
Lethality acts in the third larval instar. CkIIαTik dominantly lengthens circadian period by nearly three hours. CkIIαTik heterozygotes exhibit reduced kinase activity. In CkIIαTik there are delays in the disappearance of the products of the per and tim genes, suggesting a role in clock protein turnover.
Heterozygotes have a circadian locomotor activity period of 26.4 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies).
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
CkIIαTik/CkIIalpha[+] is an enhancer of visible phenotype of Scer\GAL4C-765, smoPKA12.UAS
CkIIαTik/CkIIalpha[+] is an enhancer of locomotor rhythm defective phenotype of per01, perS149-151-153A.tG
Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Over 60% of eggs laid by CkIIαTik/orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343 females have ventralised chorions. CkIIβmbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb8E2Hd.Hsp83.T:Ecol\lacZ orbF343/+ females to approximately 35%.
CkIIαTik/+ enhances the wing phenotype seen when smoPKA12.Scer\UAS is expressed under the control of Scer\GAL4C-765.
The increased periodicity of circadian locomoter acticvity seen in CkIIαTik/Y adults is not suppressed by S6kIIign-Δ58-1/Y.
CkIIαTik, E(spl)1 double mutant flies do not show ommatidial or interommatidial bristle defects.
The longer periods of circadian locomotor activity seen in per01; perS149-151-153A.tG flies are lengthened further when these flies are CkIIαTik/+.
pers ; CkIIαTik/+ flies have a circadian locomotor activity period of 20.4 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies). perL1 ; CkIIαTik/+ flies have a circadian locomotor activity period of 32.5 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies).
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
Based on heterozygous lethality, these alleles of CkIIα can be placed in the following order, from severe to weak: CkIIαP2 = CkIIαTikR > CkIIαP1 > CkIIαTik > CkIIαH3091 > CkIIαG703.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Timekeeper
Secondary FlyBase IDs
    References (9)