Allele Dmel\Cam^Scer\UAS.cWa

General Information
Symbol	Dmel\Cam^Scer\UAS.cWa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Wang	FlyBase ID	FBal0141860
Feature type	allele	Associated gene	Dmel\Cam

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Wang et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of wild type Cam. (Wang et al., 2002)
Carried in construct	P{UAS-Cam.W} (Wang et al., 2002, Wang et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective, with Cam⁷, Camⁿ³³⁹, Scer\GAL4^how-24B (Wang et al., 2003) behavior defective, with Cam⁷/Camⁿ³³⁹, Scer\GAL4^how-24B (Wang et al., 2003) mating defective, with Cam⁷, Camⁿ³³⁹, Scer\GAL4^how-24B (Wang et al., 2003) mating defective, with Cam⁷/Camⁿ³³⁹, Scer\GAL4^how-24B (Wang et al., 2003)
Phenotype Manifest In

Detailed Description
	Statement Reference Cam⁷/Camⁿ³³⁹ animals rescued to adulthood by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B are behaviourally abnormal; they fail to climb after being gently knocked to the bottom of a vial, and they show poor flight and a reduced ability to right themselves after a brief vortexing. The initial stages of male courtship are normal, but the rescued males cannot bend their abdomens sufficiently to achieve penetration. (Wang et al., 2003)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^how-24B, Cam^Scer\UAS.cWa, Scer\GAL4^how-24B rescues Cam⁷/Camⁿ³³⁹ (Wang et al., 2002)
Partially rescues	Cam^Scer\UAS.cWa/Scer\GAL4^elav.PLu partially rescues Camⁿ³³⁹ (Wang et al., 2003) Scer\GAL4^how-24B, Cam^Scer\UAS.cWa, Scer\GAL4^how-24B partially rescues Cam⁷/Camⁿ³³⁹ (Wang et al., 2003)
Fails to rescue	Cam^Scer\UAS.cWa/Scer\GAL4^elav.PLu fails to rescue Cam⁷/Camⁿ³³⁹ (Wang et al., 2003) Scer\GAL4^how-24B, Cam^Scer\UAS.cWa, Scer\GAL4^how-24B fails to rescue Camⁿ³³⁹ (Wang et al., 2003) Scer\GAL4^sca.PU/Cam^Scer\UAS.cWa fails to rescue Cam⁷/Camⁿ³³⁹ (Wang et al., 2003) Scer\GAL4^sca.PU/Cam^Scer\UAS.cWa fails to rescue Camⁿ³³⁹ (Wang et al., 2003)
Comments	The lethality and pupariation defects seen in Cam⁷/Camⁿ³³⁹ animals are rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B; the pupal cases are morphologically wild type and have more normal length:width ratios, almost all of the rescued pupae eclose and no aberrant pharate head structures are seen. The lethality and pupariation defects seen in Cam⁷/Camⁿ³³⁹ animals are not rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu or Scer\GAL4^sca.PU. The sluggish larval locomotion of Cam⁷/Camⁿ³³⁹ animals is incompletely rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B or Scer\GAL4^elav.PLu. The spontaneous backward movement seen in homozygous Camⁿ³³⁹ larvae is not rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B or Scer\GAL4^sca.PU. The spontaneous backward movement seen in homozygous Camⁿ³³⁹ larvae is rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu, although the larvae are still sluggish. (Wang et al., 2003) Expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B rescues the developmental defects and lethality of Cam⁷/Camⁿ³³⁹ mutants. (Wang et al., 2002)
Stocks ( 1 )
Bloomington	6809 y¹ w^*; Camⁿ³³⁹/CyO, y⁺; P{UAS-Cam.W}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Cam^Scer\UAS.cWa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Wang (Wang et al., 2002)
Secondary FlyBase IDs

References ( 2 )
Research paper	Wang et al., 2003, Genetics 165(3): 1255--1268 Drosophila calmodulin mutants with specific defects in the musculature or in the nervous system. [FBrf0167631] Wang et al., 2002, genesis 34(1-2): 86--90 Calmodulin UAS-constructs and the in vivo roles of calmodulin: analysis of a muscle-specific phenotype. [FBrf0152385]

Allele Dmel\CamScer\UAS.cWa

Allele Dmel\Cam^Scer\UAS.cWa