Allele Dmel\Cam^{B12Q.Scer\UAS}

General Information
Symbol	Dmel\Cam^{B12Q.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0141862
Feature type	allele	Associated gene	Dmel\Cam

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Wang et al., 2002) in vitro construct - regulatory fusion (Wang et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: E?Q. Scer\UAS regulatory sequences drive expression of a mutated form of Cam in which the two N-terminal Ca²⁺ binding sites have been inactivated. (Wang et al., 2002)
Carried in construct	P{UAS-Cam.B12Q} (Wang et al., 2002, Wang et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Cam^{B12Q.Scer\UAS} under the control of Scer\GAL4^how-24B in Cam⁷/+ animals (which are normally phenotypically wild type) has no effect on pupal case structure or survival. Expression of both Cam^{B12Q.Scer\UAS} and Cam^{B34Q.Scer\UAS} under the control of Scer\GAL4^how-24B in Cam⁷/Camⁿ³³⁹ animals results in 100% larval lethality. (Wang et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^how-24B, Scer\GAL4^how-24B, Cam^{B12Q.Scer\UAS} partially rescues Cam⁷/Camⁿ³³⁹ (Wang et al., 2002)
Fails to rescue	Cam^{B12Q.Scer\UAS}/Scer\GAL4^elav.PLu fails to rescue Camⁿ³³⁹ (Wang et al., 2003)
Comments	(with Cam^{B34Q.Scer\UAS}) Fails to rescue: Camⁿ³³⁹ { Scer\GAL4^elav.PLu } The spontaneous backward movement seen in homozygous Camⁿ³³⁹ larvae is not rescued by expression of Cam^{B12Q.Scer\UAS} under the control of Scer\GAL4^elav.PLu. The spontaneous backward movement seen in homozygous Camⁿ³³⁹ larvae is not rescued by co-expression of Cam^{B12Q.Scer\UAS} and Cam^{B34Q.Scer\UAS} under the control of Scer\GAL4^elav.PLu. (Wang et al., 2003) Expression of Cam^{B12Q.Scer\UAS} under the control of Scer\GAL4^how-24B rescues the overcontraction of the larval body seen during pupariation in Cam⁷/Camⁿ³³⁹ animals and produces smooth pupal cases, but the pupal cases are longer and narrower than normal and the anterior larval segments do not retract fully during pupariation. Most of the pupae fail to develop recognisable adult structures and only 9.4% of the pupae eclose. (Wang et al., 2002)
Stocks ( 1 )
Bloomington	6808 y¹ w^*; Camⁿ³³⁹/CyO, y⁺; P{UAS-Cam.B12Q}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Cam^{B12Q.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wang et al., 2003, Genetics 165(3): 1255--1268 Drosophila calmodulin mutants with specific defects in the musculature or in the nervous system. [FBrf0167631] Wang et al., 2002, genesis 34(1-2): 86--90 Calmodulin UAS-constructs and the in vivo roles of calmodulin: analysis of a muscle-specific phenotype. [FBrf0152385]

Allele Dmel\CamB12Q.Scer\UAS

Allele Dmel\Cam^{B12Q.Scer\UAS}