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General Information
Symbol
Dmel\Atx2X1
Species
D. melanogaster
Name
FlyBase ID
FBal0141942
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Datx2X1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{EP} element, resulting in a 1.4kb deletion that removes the first 22 codons of the Atx2 coding sequence and extends into the first intron.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Atx2X1 adult antennal lobe projection neuron clones are of comparable size to control clones, but display far less stringent targeting of dendrites, growing across glomeruli borders and innervating multiple glomeruli as opposed to just one.

Adult Atx2X1/Atx206490 transheterozygotes also bearing either Atx2ΔmIDR or Atx2ΔcIDR, but not Atx2+t10.4, exhibit significantly lower long-term olfactory habituation, as compared to controls.

Atx2X1 mutant sensory neuron clones in the anterior wing margin (generated using MARCM) are retained at a similar rate to wild type at 6 days (83% in wild type and 75% in homozygous mutants).

Adult Atx2X1 heterozygotes do not show any (age-related or otherwise) impairment in their climbing abilities compared to controls.

Hemizygotes show second instar larval lethality. Homozygous clones in the retina result in severe loss of eye tissue. Eye discs of third instar larvae carrying homozygous retina clones are smaller than normal and have a disorganised ommatidial structure. Increased apoptosis is seen in these eye discs. Females carrying homozygous germ line clones are completely sterile and fail to lay eggs. Egg chambers in these females arrest prior to the cytoplasmic transport stage. The oocyte is often found in the middle of the egg chamber, or the oocyte is not specified at all. Columnar follicle cells are present in the vicinity of misplaced oocytes and the border cells migrate towards the misplaced oocytes. Atx2X1 flies rescued by Atx2Scer\UAS.cSa (in the absence of a Scer\GAL4 driver) are uncoordinated, can have a rough eye phenotype and have bent and forked thoracic sensory bristles. Many of the bristles have highly disorganised cuticular ridges and the actin bundles are shorter and thinner than normal. Homozygous clones result in bristles with shorter and thinner actin bundles than normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

Atx2X1/Atx2[+], Fmr13 has learning defective | dominant phenotype, suppressible by Atx2+t10.4

Atx2X1/Atx2[+], Fmr13 has learning defective | dominant phenotype, suppressible by Fmr1+t14

AGO1k08121/AGO1[+], Atx2X1 has learning defective | dominant phenotype, suppressible by Atx2+t10.4

Atx2X1, me31B[+]/me31BΔ2 has learning defective | dominant phenotype, suppressible by Atx2+t10.4

Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Fmr13, Atx2X1 double heterozygotes exhibit a complete block of the long-term habituation (LTH) normally seen when flies are exposed to either ethyl butyrate (EB) or CO[[2]]] for 4 days. LTH appears normal in either heterozygote alone. This loss of EB-evoked LTH is restored by expression of either Atx2+t10.4 or Fmr1+t14. Short term habituation following one hour exposure to EB appears normal.

AGO1k08121, Atx2X1 double heterozygotes exhibit a complete block of the long-term habituation (LTH) seen when flies are exposed to either ethyl butyrate (EB) or CO[[2]]] for 4 days. LTH appears normal in either heterozygote alone. This loss of EB-evoked LTH is restored by expression of Atx2+t10.4. Short term habituation following one hour exposure to EB appears normal. LTH-associated structural plasticity is also blocked: although the V and DM5 glomeruli of Atx2X1/+ flies show the expected growth following 4d of CO[[2]] or EB exposure, respectively, both the EB-evoked increase in DM5 volume and the CO[[2]]-induced increase in V are abolished in AGO1k08121/+; Atx2X1/+ double heterozygotes. These defects in structural plasticity are restored by Atx2+t10.4.

me31BΔ2, Atx2X1 double heterozygotes exhibit a complete block of the long-term habituation (LTH) seen when flies are exposed to either ethyl butyrate (EB) or CO[[2]]] for 4 days. LTH appears normal in either heterozygote alone. This loss of EB-evoked LTH is restored by expression of Atx2+t10.4. Short term habituation following 1 hour exposure to EB appears normal. LTH-associated structural plasticity is also blocked: although the V and DM5 glomeruli of Atx2X1/+ flies show the expected growth following 4d of CO[[2]] or EB exposure, respectively, both the EB-evoked increase in DM5 volume and the CO[[2]]-induced increase in V are abolished in me31BΔ2/+; Atx2X1/+ double heterozygotes. These defects in structural plasticity are restored by Atx2+t10.4.

Xenogenetic Interactions
Statement
Reference

One copy of Atx2X1 suppresses the eye degeneration seen when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed in differentiated photoreceptor neurons under the control of Scer\GAL4ninaE.PD. On average 6.9 visible photoreceptors are seen per ommatidium in 12 day old flies, similar to the 7 seen in controls. This compares to 6.4 when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed alone.

Homozygous Atx2X1 suppresses the neuron loss seen in anterior wing margin clones induced in flies expressing Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155 (generated using MARCM). 77% of neurons are retained at 3 days, compared to only 13% when Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 is expressed alone. 40% are recovered at 6 days, in contrast to the complete loss of neurons in Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 expressing flies.

The age-progressive locomotor impairment and shorter adult life span characteristic for flies expressing Hsap\ATXN182Q.Scer\UAS under the control of Scer\GAL4nrv2.PU as well as the loss of macrochaeta observed in flies expressing Hsap\ATXN182Q.Scer\UAS under the Scer\GAL4sca-109-68 driver are significantly improved by combination with a Atx2X1 in a heterozygous state.

Neither the age-progressive retina degeneration nor the decline of climbing abilities characteristic for adult flies expressing Hsap\HTTNT.128Q.Scer\UAS under the control of the Scer\GAL4GMR.PU or the Scer\GAL4nrv2.PU driver, respectively, can be suppressed by combination with Atx2X1 in a heterozygous state.

Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments

The lethality of Atx2X1/Atx206490 mutants is rescues by Atx2+t10.4, Atx2ΔmIDR, Atx2ΔcIDR and partially rescued by Atx2ΔPam2 but is not rescued by Atx2ΔLSM-Ad or Atx2ΔLSM.

The dendrite targeting defects of Atx2X1 adult antennal lobe projection neuron clones are rescued by Atx2ΔmIDR or Atx2ΔcIDR.

Expression of Atx2+t10.4 rescues the lethality of Atx2X1/Atx206490.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (8)