Allele Dmel\Apc2^f90

General Information
Symbol	Dmel\Apc2^f90	Species	D. melanogaster
Name		FlyBase ID	FBal0141948
Feature type	allele	Associated gene	Dmel\Apc2

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Akong et al., 2002)
Mutagen	ethyl methanesulfonate (Akong et al., 2002, McCartney et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q406Q. (McCartney et al., 2006) Amino acid replacement: ??@. Stop codon in Arm repeat. (Akong et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| rescuable maternal effect (McCartney et al., 2006) viable (Akong et al., 2002)
Phenotype Manifest In
	denticle belt \| maternal effect (McCartney et al., 2006) embryonic/first instar larval cuticle \| maternal effect (McCartney et al., 2006)
Detailed Description
	Statement Reference The cuticle of an average Apc2^g41 maternal/zygotic embryo shows an anterior hole, is 50-60% the length of wild-type and has at most 2 patches of denticles remaining. (McCartney et al., 2006) Asymmetric division of neuroblasts appear to be unaffected in the brains of Apc2^g10/Apc2^f90 larvae. Apc2^f90 has a more severe maternal effect embryonic phenotype than either Apc2^Σ or Apc2^d40. (Akong et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement Reference Apc2^f90, Apc^Q8 has embryonic/larval cuticle \| germline clone \| rescuable maternal effect \| embryonic stage phenotype, non-suppressible by Axn^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^arm.PS (Mendoza-Topaz et al., 2011)
Other
Statement Reference Apc2^f90, Apc^Q8 has embryonic/larval cuticle \| germline clone \| rescuable maternal effect \| embryonic stage phenotype (Mendoza-Topaz et al., 2011) Apc2^f90/Apc2[+], Apc^Q8 has embryonic/larval cuticle \| germline clone \| rescuable maternal effect \| embryonic stage phenotype (Mendoza-Topaz et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Embryos maternally and zygotically double mutant for Apc[Q8] and Apc2[f90] display a naked cuticle phenotype. Expression of Axn[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[arm.PS] is unable to restore normal-looking denticle belts in embryos that are maternally and zygotically mutant for Apc[Q8] and Apc2[f90]. Every single embryo exhibits a completely naked cuticle, without any sign of denticle restoration. Expression of Axn[Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[arm.PS] restores normal-looking denticle belts in embryos derived from females containing Apc[Q8] Apc2[f90] double mutant germline clones crossed with wild type males. Expressing Axn[FGmut.Scer\UAS.T:Avic\GFP] under the control of Scer\GAL4[arm.PS] does not suppress the naked cuticle phenotype seen in Apc[Q8] Apc2[f90] double mutant embryos. Only occasional individual denticles are seen. (Mendoza-Topaz et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Apc2^ΔS (Akong et al., 2002)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Apc2 alleles can be divided into three categories based on their embryonic cuticle phenotypes, from weak to moderate to strong: Apc2^e90 = Apc2^b5 = Apc2^N175K < Apc2^c9 = Apc2^ΔS = Apc2^d40 < Apc2^g41 = Apc2^f90 = Apc2^g10. (McCartney et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	APC2^f90 (Akong et al., 2002, McCartney et al., 2006) Apc2^f90 (Takacs et al., 2008) e-apc^f90 (Mendoza-Topaz et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Mendoza-Topaz et al., 2011, Open Biol. 1(3): 110013 The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled. [FBrf0218486] McCartney et al., 2006, Development 133(12): 2407--2418 Testing hypotheses for the functions of APC family proteins using null and truncation alleles in Drosophila. [FBrf0192526] Akong et al., 2002, Dev. Biol. 250(1): 71--90 Drosophila APC2 and APC1 have overlapping roles in the larval brain despite their distinct intracellular localizations. [FBrf0151885]
Supplementary material	Takacs et al., 2008, Science 319(5861): Supporting online material. [FBrf0202572]

Allele Dmel\Apc2f90

Allele Dmel\Apc2^f90