Allele Dmel\mts^{dn181.Scer\UAS}

General Information
Symbol	Dmel\mts^{dn181.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0143316
Feature type	allele	Associated gene	Dmel\mts

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hannus et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a dominant negative form of mts (an N-terminally truncated form starting at amino acid 181). (Hannus et al., 2002)
Carried in construct	P{UAS-mts.dn181} (Hannus et al., 2002, Sathyanarayanan et al., 2004, Viquez et al., 2006, Chabu and Doe, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number, with Scer\GAL4^Act.PU (Chabu and Doe, 2009) locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf (Sathyanarayanan et al., 2004) locomotor rhythm defective, with Scer\GAL4^tim.PE (Sathyanarayanan et al., 2004) neuroanatomy defective, with Scer\GAL4^elav.PLu (Viquez et al., 2006) planar polarity defective, with Scer\GAL4^ptc-559.1 (Hannus et al., 2002)
Phenotype Manifest In
	bouton & neuromuscular junction \| larval stage, with Scer\GAL4^elav.PLu (Viquez et al., 2006) embryonic/larval neuroblast, with Scer\GAL4^Act.PU (Chabu and Doe, 2009) microtubule & neuromuscular junction \| larval stage, with Scer\GAL4^elav.PLu (Viquez et al., 2006) wing hair, with Scer\GAL4^ptc-559.1 (Hannus et al., 2002)
Detailed Description
	Statement Reference mts[dn181.Scer\UAS] under the control of Scer\GAL4[Act] results in an increase in neuroblast numbers compared to controls. (Chabu and Doe, 2009) Larvae expressing mts[dn181.Scer\UAS] under the control of Scer\GAL4[elav.PLu] have aberrant bouton morphology and size, and disruption to the NMJ cytoskeleton, with an 88% increase in the levels of unbundled microtubules relative to wild-type. Boutons are observed filled with an unusually high number of splayed filaments. (Viquez et al., 2006) Flies expressing mts^{dn181.Scer\UAS} under the control of Scer\GAL4^tim.PE are arrhythmic with respect to locomotor activity. Flies expressing mts^{dn181.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf show an increase in the period of locomotor activity rhythms compared to controls. The rhythms appear to be unstable; some flies have long periods with interspersed days of prolonged activity and others show multiple periodicities. (Sathyanarayanan et al., 2004) Expression of mts^{dn181.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 results in a lack of wing hairs over a significant region of the anterior-posterior compartment boundary. In regions where hairs are present, they often have abnormal orientation. (Hannus et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^elav.PLu, mts^{dn181.Scer\UAS} has neuroanatomy defective phenotype, enhanceable by PP2A-B'[+]/PP2A-B'¹⁰⁴, Scer\GAL4^elav.PLu (Viquez et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^elav.PLu, mts^{dn181.Scer\UAS} has microtubule & neuromuscular junction \| larval stage phenotype, enhanceable by PP2A-B'[+]/PP2A-B'¹⁰⁴, Scer\GAL4^elav.PLu (Viquez et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference A PP2A-B'[104] heterozygote background significantly enhances the unbundling microtubule phenotype of neuronal mts[dn181.Scer\UAS] expression (under the control of Scer\GAL4[elav.PLu]), leading to a 46% increase in unbundled microtubules relative to mts[dn181.Scer\UAS] expression alone. In addition, the cytoskeletal morphology of these double mutants is more severely disrupted than in mts[dn181.Scer\UAS] mutants. Simultaneously inhibiting mts and PP2A-B' results in synapses in which microtubules are splayed or punctate through the normally stable core region of the synaptic terminal. (Viquez et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mts^{dn181.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Chabu and Doe, 2009, Dev. Biol. 330(2): 399--405 Twins/PP2A regulates aPKC to control neuroblast cell polarity and self-renewal. [FBrf0207940] Viquez et al., 2006, J. Neurosci. 26(36): 9293--9303 The B' protein phosphatase 2A regulatory subunit well-rounded regulates synaptic growth and cytoskeletal stability at the Drosophila neuromuscular junction. [FBrf0193166] Sathyanarayanan et al., 2004, Cell 116(4): 603--615 Posttranslational regulation of Drosophila PERIOD protein by protein phosphatase 2A. [FBrf0174437] Hannus et al., 2002, Development 129(14): 3493--3503 Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A. [FBrf0151263]

Allele Dmel\mtsdn181.Scer\UAS

Allele Dmel\mts^{dn181.Scer\UAS}