All three l(2)gl phosphorylation sites have been mutated to Alanines; the changes are S656A, S660A and S664A.
Expression of l(2)gl3A.Scer\UAS under the control of Scer\GAL4sca.PU in the adult thorax leads to an increase in the frequency of bristle twinning, as compared to controls.
Larval neuroblasts expressing l(2)gl3A.Scer\UAS via Scer\GAL4wor.PA show abnormal localization of cell polarity determinants.
Scer\GAL4neur-P72-mediated expression of l(2)gl3A.Scer\UAS (controlled using Scer\GAL80ts.αTub84B) abolishes the asymmetric targeting of Sara-positive endosomes during asymmetric cell division of SOPs.
Expression of l(2)gl3A.Scer\UAS under the control of Scer\GAL4wor.PA has no affect on the number of neuroblasts in the larval brain.
In 6% of sensory organ precursor cells expressing l(2)gl3A.Scer\UAS under the control of Scer\GAL4sca-109-68, pIIb cells are transformed into pIIa cells.
Expression of l(2)gl3A.Scer\UAS by Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not affect spindle orientation of embryonic neuroblasts.
Scer\GAL4wor.PA, l(2)gl3A.UAS has abnormal cell polarity phenotype, suppressible by Scer\GAL4wor.PA/aPKCΔN.UAS
Scer\GAL4sca.PU, l(2)gl3A.UAS has mesothoracic bristle | increased number | adult stage phenotype, enhanceable by RhebUAS.cPa, Scer\GAL4sca.PU
Scer\GAL4sca-109-68, l(2)gl3A.UAS has external sensory organ precursor cell IIb phenotype, enhanceable by Scer\GAL4sca-109-68/nufEP3324
Scer\GAL4sca-109-68, l(2)gl3A.UAS has external sensory organ precursor cell IIa phenotype, enhanceable by Scer\GAL4sca-109-68/nufEP3324
Scer\GAL4wor.PA, l(2)gl3A.UAS has larval neuroblast phenotype, suppressible by Scer\GAL4wor.PA/aPKCΔN.UAS
l(2)gl3A.UAS/Scer\GAL4sca-109-68 is an enhancer of trichogen cell phenotype of Scer\GAL4sca-109-68, nufEP3324
l(2)gl3A.UAS/Scer\GAL4sca-109-68 is an enhancer of tormogen cell | increased number phenotype of Scer\GAL4sca-109-68, nufEP3324
l(2)gl3A.UAS/Scer\GAL4wor.PA is a suppressor of larval brain & neuroblast | supernumerary phenotype of Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk)
Co-expression of RhebScer\UAS.cPa enhances the bristle twinning phenotype seen in flies expressing l(2)gl3A.Scer\UAS under the control of Scer\GAL4sca.PU.
Co-expression of aPKCΔN.Scer\UAS results in normal localization of cell polarity determinants in larval neuroblasts expressing l(2)gl3A.Scer\UAS via Scer\GAL4wor.PA.
Co-expression of l(2)gl3A.Scer\UAS strongly suppresses the increased number of neuroblasts in the larval brain caused by expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA.
Sensory organ precursor cells expressing l(2)gl3A.Scer\UAS and nufEP3324 under the control of Scer\GAL4sca-109-68 show an enhancement of the pIIb to pIIa transformation seen when only l(2)gl3A.Scer\UAS is expressed and an enhancement of the hair to socket transformation seen when only nufEP3324 is expressed.