FlyBase Allele Report: Dmel\l(2)gl[3A.UAS]

General Information

Symbol

Dmel\l(2)gl^3A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0143420

Feature type

allele

Associated gene

Dmel\l(2)gl

Associated Insertion(s)

Carried in Construct

P{UAS-l(2)gl.3A}

Also Known As

lgl3A, UAS-lgl3A, UAS-Lgl^3A

Key Links

Allele class

Mutagen

in vitro construct - site directed mutagenesis

Nature of the Allele

Allele class

Mutagen

in vitro construct - site directed mutagenesis

(Betschinger et al., 2003)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

l(2)gl^UAS.cBa

(Betschinger et al., 2003)

Carried in construct

P{UAS-l(2)gl.3A}

Cytology

Nature of the lesion

Statement

Reference

All three l(2)gl phosphorylation sites have been mutated to Alanines; the changes are S656A, S660A and S664A.

(Betschinger et al., 2003)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

l(2)gl

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^wor.PA

(Atwood and Prehoda, 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of l(2)gl^3A.Scer\UAS under the control of Scer\GAL4^sca.PU in the adult thorax leads to an increase in the frequency of bristle twinning, as compared to controls.

(Karbowniczek et al., 2010)

Larval neuroblasts expressing l(2)gl^3A.Scer\UAS via Scer\GAL4^wor.PA show abnormal localization of cell polarity determinants.

(Atwood and Prehoda, 2009)

Scer\GAL4^neur-P72-mediated expression of l(2)gl^3A.Scer\UAS (controlled using Scer\GAL80^ts.αTub84B) abolishes the asymmetric targeting of Sara-positive endosomes during asymmetric cell division of SOPs.

(Coumailleau et al., 2009)

Expression of l(2)gl^3A.Scer\UAS under the control of Scer\GAL4^wor.PA has no affect on the number of neuroblasts in the larval brain.

(Lee et al., 2006)

In 6% of sensory organ precursor cells expressing l(2)gl^3A.Scer\UAS under the control of Scer\GAL4^sca-109-68, pIIb cells are transformed into pIIa cells.

(Emery et al., 2005)

Expression of l(2)gl^3A.Scer\UAS by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not affect spindle orientation of embryonic neuroblasts.

(Betschinger et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, l(2)gl^3A.UAS has abnormal cell polarity phenotype, suppressible by Scer\GAL4^wor.PA/aPKC^ΔN.UAS

(Atwood and Prehoda, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sca.PU, l(2)gl^3A.UAS has mesothoracic bristle | increased number | adult stage phenotype, enhanceable by Rheb^UAS.cPa, Scer\GAL4^sca.PU

(Karbowniczek et al., 2010)

Scer\GAL4^sca-109-68, l(2)gl^3A.UAS has external sensory organ precursor cell IIb phenotype, enhanceable by Scer\GAL4^sca-109-68/nuf^EP3324

(Emery et al., 2005)

Scer\GAL4^sca-109-68, l(2)gl^3A.UAS has external sensory organ precursor cell IIa phenotype, enhanceable by Scer\GAL4^sca-109-68/nuf^EP3324

(Emery et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, l(2)gl^3A.UAS has larval neuroblast phenotype, suppressible by Scer\GAL4^wor.PA/aPKC^ΔN.UAS

(Atwood and Prehoda, 2009)

Enhancer of

Statement

Reference

l(2)gl^3A.UAS/Scer\GAL4^sca-109-68 is an enhancer of trichogen cell phenotype of Scer\GAL4^sca-109-68, nuf^EP3324

(Emery et al., 2005)

l(2)gl^3A.UAS/Scer\GAL4^sca-109-68 is an enhancer of tormogen cell | increased number phenotype of Scer\GAL4^sca-109-68, nuf^EP3324

(Emery et al., 2005)

Suppressor of

Statement

Reference

l(2)gl^3A.UAS/Scer\GAL4^wor.PA is a suppressor of larval brain & neuroblast | supernumerary phenotype of Scer\GAL4^wor.PA, aPKC^{UAS.Tag:CAAX(Unk)}

(Lee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rheb^Scer\UAS.cPa enhances the bristle twinning phenotype seen in flies expressing l(2)gl^3A.Scer\UAS under the control of Scer\GAL4^sca.PU.

(Karbowniczek et al., 2010)

Co-expression of aPKC^ΔN.Scer\UAS results in normal localization of cell polarity determinants in larval neuroblasts expressing l(2)gl^3A.Scer\UAS via Scer\GAL4^wor.PA.

(Atwood and Prehoda, 2009)

Co-expression of l(2)gl^3A.Scer\UAS strongly suppresses the increased number of neuroblasts in the larval brain caused by expression of aPKC^{CAAXWT.Scer\UAS} under the control of Scer\GAL4^wor.PA.

(Lee et al., 2006)

Sensory organ precursor cells expressing l(2)gl^3A.Scer\UAS and nuf^EP3324 under the control of Scer\GAL4^sca-109-68 show an enhancement of the pIIb to pIIa transformation seen when only l(2)gl^3A.Scer\UAS is expressed and an enhancement of the hair to socket transformation seen when only nuf^EP3324 is expressed.

(Emery et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Lgl3A

(Zarnescu et al., 2007)

l(2)gl^3A.Scer\UAS

l(2)gl^3A.UAS

lgl3A

(Langevin et al., 2005, Mayer et al., 2005)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Betschinger

(Betschinger et al., 2003)

Secondary FlyBase IDs

References (15)

Report Sections

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