A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\SmBSH0509

General Information
SymbolDmel\SmBSH0509SpeciesD. melanogaster
NameFlyBase IDFBal0143984
Feature typealleleAssociated geneDmel\SmB
Map ( GBrowse ) Untitled Document detailed view FBti0026206 FBti0021324 FBti0151519
Allele class
MutagenP-element activity
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Description
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Nature of the Allele
Allele class
Mutagen
P-element activity
(Oh et al., 2003)
Mutations Mapped to the Genome
Type
Location
Additional Notes
References
transposable element insertion site
2L:10,426,039..10,426,225
 
(Oh et al., 2003, Anne, 2010)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion
Statement
Reference
 
 
Caused by insertion
P{lacW}SmBSH0509
(Oh et al., 2003, Anne, 2010)
Cytology
hide Phenotypic Data
hide Phenotypic Class
lethal | recessive
(Oh et al., 2003)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
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Linkouts
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hide Phenotypic Class
hide Phenotype Manifest In
hide Additional Comments
hide Genetic Interactions
Statement
Reference
hide Xenogenetic Interactions
Statement
Reference
hide Complementation & Rescue Data
Partially rescued by
SmBSH0509/SmBBG02775 is partially rescued by Scer\GAL4tub.PU/SmBR1-3K.Scer\UAS.P\T
(Anne, 2010)
SmBSH0509/SmBBG02775 is partially rescued by Scer\GAL4tub.PU/SmBR4-7.Scer\UAS.P\T
(Anne, 2010)
SmBSH0509/SmBBG02775 is partially rescued by Scer\GAL4tub.PU/SmBScer\UAS.P\T.cAa
(Anne, 2010)
SmBSH0509/SmBBG02775 is partially rescued by SmBR1-7.Scer\UAS.P\T/Scer\GAL4tub.PU
(Anne, 2010)
SmBSH0509/SmBBG02775 is partially rescued by SmBR1-7K.Scer\UAS.P\T/Scer\GAL4tub.PU
(Anne, 2010)
SmBSH0509/SmBBG02775 is partially rescued by SmBR4-7K.Scer\UAS.P\T/Scer\GAL4tub.PU
(Anne, 2010)
Comments
Expression of SmB[Scer\UAS.P\T.cAa] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 61%. Expression of SmB[R1-7.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 32%. However, all the rescued flies are completely agametic. Although germ cells can migrate through the midgut epithelium in these embryos, they are unable to coalesce with the gonadal mesoderm. Expression of SmB[R1-7K.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 28%. However, all the rescued flies are completely agametic. Although germ cells can migrate through the midgut epithelium in these embryos, they are unable to coalesce with the gonadal mesoderm. Expression of SmB[R4-7.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 37%. Rescued females have smaller ovaries than normal and contain mostly previtellogenic egg chambers. Most egg chambers that have reached the end of previtellogenic development show signs of degeneration, including a condensed nuclear morphology. Embryos derived from the few eggs laid by the rescued females build a normal layer of blastoderm cells, but fail to form pole cells. Expression of SmB[R4-7K.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 30%. Rescued females have smaller ovaries than normal and contain mostly previtellogenic egg chambers. Most egg chambers that have reached the end of previtellogenic development show signs of degeneration, including a condensed nuclear morphology. Expression of SmB[R1-3K.Scer\UAS.P\T] under the control of Scer\GAL4[tub.PU] rescues the lethality of SmB[BG02775]/SmB[SH0509] with an efficiency of 27%. The rescued animals have no gametes.
(Anne, 2010)
hide Stocks ( 1 )
Kyoto
122076
w*; P{lacW}SmBSH0509
hide Notes on Origin
Discoverer
Separable from: a second-site lethal mutation.
(Anne, 2010)
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Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym
l(2)SH0509SH0509
 
SmBl(2)SH0509
(Anne, 2010)
SmBSH0509
 
Name Synonym
Secondary FlyBase IDs
hide References ( 2 )
Research paper
Anne, 2010, Development 137(17): 2819--2828
Arginine methylation of SmB is required for Drosophila germ cell development. [FBrf0211523]
Supplementary material
Oh et al., 2003, Genetics 163(1):
Table S1. [FBrf0157097]