jebSH0422 mutant embryos show visceral muscle fusion defects and an absence of Fas3-positive gut structures.
R cells, target neurons and glia are normal in jebSH0422 eye mosaic clones.
Third larval instar animals with jebSH0422 clones in the eye disc show normal R cell axon targeting. Adults that contain jebSH0422 clones in the eye exhibit strong R cell projection defects. R8 axons frequently extend to the M6 layer, fail to enter the medulla or fasciculate with R8 axons in neighbouring columns. Analysis of jebSH0422 mosaics at pupal stages shows that the R cell projection errors begin in the medulla at around 55 hours APF.
jebSH0422 clones in lamina target neurons do not affect the projection of R cells.
The size, shape and array of lamina cartridges are irregular in adults that lack jeb function due to jebSH0422 clones in the eye. These lamina cartridges contain either less or more than the wild-type R cell profile. Extensions of R1-R6 axons projecting from individual ommatidia appear irregular and shortened; some R1-R6 axons appear not to defasciculate and other growth cones extend at shorter distances.