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General Information
Symbol
Dmel\grhUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hemphala
FlyBase ID
FBal0144274
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-grh
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the N'-form of grh.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of grhScer\UAS.cHa in the amnioserosa under the control of Scer\GAL4c381 or Scer\GAL4332.3 is sufficient to block dorsal closure in 100% and 50% of embryos, respectively. Defects are evident from stage 14-16. The amnioserosa is less contracted than in wild-type and contains cells with abnormal morphology. The epidermal edges fail to meet at the poles during dorsal closure. In embryos over-expressing grhScer\UAS.cHa under the control of Scer\GAL4c381, there are large variations in shape and size of amnioserosa cells, and the contacts with adjacent dorsal epidermis cells is dramatically different. Some epidermal cells expand contact with an amnioserosa cell at the expense of neighbouring cells which become bunched together.

The visceral and dorsal branches of the tracheal system only form rudimentary buds in embryos expressing grhScer\UAS.cHa under the control of Scer\GAL4btl.PS, and the dorsal and lateral trunk branches in each hemisegment remain unconnected at stage 16. Fusion cell differentiation is not affected, but they fail to form interconnecting sprouts. No secondary branch lumen is formed. The basolateral projections of tracheal cells are unaffected in these embryos, but their apical surface does not elongate to form a branch. Expression of grhScer\UAS.cHa under the control of Scer\GAL4bs-23.26 prevents the formation and elongation of the intracellular lumen in terminal cells of the tracheal system.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
grhScer\UAS.cHa
grhUAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hemphala
Secondary FlyBase IDs
    References (5)