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General Information
Symbol
Dmel\mir-banEP3622
Species
D. melanogaster
Name
FlyBase ID
FBal0144429
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
banEP3622, EP(3)3622
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The "EP(3)3622" line contains two P{EP} elements (P{EP}banEP3622a and P{EP}banEP3622b) inserted in a back-to-back orientation at 61C in contig AE003469.
Insertion components
P{EP}EP3622a
Product class / Tool use(s)
Encoded product / tool
P{EP}EP3622b
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of banEP3622 under the control of Scer\GAL4ptc.PU in cells of the wing disc proper has no significant effect on the morphology or proliferation of the overlying peripodial membrane cells in third instar larvae.
Expression of banEP3622 under the control of Scer\GAL4tim.PE results in a dramatically lengthened circadian period when locomotor activity is assayed (the increase is almost 3 hours). Expression of banEP3622 under the control of Scer\GAL4P2.4.Pdf results in a lengthened circadian period when locomotor activity is assayed.
banEP3622 eye disc clones show no cell death.
banEP3622 homozygotes or banEP3622/Df(3L)banΔ1 hemizygotes survive to adulthood and show wild-type levels of apoptosis. Imaginal discs in banEP3622 homo- and hemizygotes or banEP3622/banL1170a transheterozygotes show significantly higher levels of AO staining than wild-type, indicating that these mutants undergo IR-induced apoptosis more readily than wild-type. Phenotypes for banEP3622 homozygotes are more severe than those of banEP3622/Df(3L)banΔ1 hemizygotes. banEP3622 homozygotes, banEP3622/banL1170a transheterozygotes and banEP3622/Df(3L)banΔ1 hemizygotes exposed to 0-8000 R of X-rays show decreased survival to adulthood after irradiation.
banL1170a/banEP3622 mutant germline stem cells (GSCs) show maintenance and cell division defects when clones are generated during adult stages. When banL1170a/banEP3622 clones are generated in larval and pupal stages the GSC loss is less severe. banEP3622/Df(3L)banΔ1 mutant germline stem cells (GSCs) show maintenance and cell division defects when clones are generated during adult stages. When banEP3622/Df(3L)banΔ1 clones are generated in larval and pupal stages the GSC loss is less severe.
Expression of banEP3622 in the developing eye, under the control of Scer\GAL4GMR.PF, causes the generation of extra interommatidial pigment and bristle cells.
Expression of banEP3622 in the eye, driven by Scer\GAL4GMR.PF, leads to eye overgrowth, due mainly to excess interommatidial cells. The number of cells undergoing apoptosis is reduced in these eyes, but this reduction is not great enough to account for all of the extra cells. The morphology of the eye is not affected.
Cells in the posterior compartment of the wing are 30.1% smaller than in controls in flies expressing banEP3622 under the control of Scer\GAL4en-e16E. The area of the posterior compartment is increased by 19.1% in these flies compared to controls, and the number of cells in the posterior compartment is increased by 68.7%. The number of cells in the anterior compartment in flies expressing banEP3622 under the control of Scer\GAL4en-e16E is reduced by 12.7% compared to controls. Expression of banEP3622 under the control of Scer\GAL4ap-md544 results in a large increase in notum size and reduction in cell size in the notum. The density of chaetae in the acrostical region is increased by 17.5% compared to controls and is associated with a lower number of cells between microchaetae (4.52 compared to 5.34 in wild type). The total number of chaetae per heminotum is increased by 49% compared to wild type.
The body mass of banEP3622/Df(3L)emc flies is 68% that of control flies. The wing blade surface area is 84.3% that of controls. The body mass of banEP3622/Df(3L)banΔ1 flies is 80% that of control flies. The wing blade surface area is 89.6% that of controls. Cell size in the wing blade is 99.2% of controls and cell number in the wing blade is 91.1% that of controls. Heterozygotes have a normal body mass compared to controls. The body mass of homozygotes is 82% that of control flies. The wing blade surface area is 91.2% that of controls. Cell size in the wing blade is 100% of controls and cell number in the wing blade is 91.1% that of controls. The body mass of banEP3622/banUY3207 flies is 84% that of control flies. The wing blade surface area is 92.6% that of controls. Cell size in the wing blade is 99.3% of controls and cell number in the wing blade is 92.8% that of controls.
The body mass of banEP3622/Df(3L)banΔ1 adults is only 78% of that of control flies. The body mass of homozygous adults is only 80% of that of control flies. The wing blade area of banEP3622/Df(3L)banΔ1 flies is only 90% of that of controls. This decrease is entirely due to a decrease in the number of cells, and is not due to a decrease in cell size. Expression of banEP3622 under the control of Scer\GAL4en-e16E results in a significant increase in the ratio of posterior:anterior area of the wing compared to control wings. There is a statistically significant increase in the overall size of the wing compared to controls. Expression of banEP3622 under the control of Scer\GAL4GMR.PF results in roughening and bulging of the eye, suggesting extensive outgrowth. Clones of cells expressing banEP3622 under the control of Scer\GAL4Act5C.PP show the same distribution of cells in G1, S and G2 phases compared to wild-type cells, but the cells are 3% larger than the control cells. The cell doubling time is significantly reduced compared to controls. Expression of banEP3622 under the control of Scer\GAL4Bx-MS1096 results in significant outgrowth of the entire wing, with the wings curving downwards.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
One copy of Df(3L)Exel6115 suppresses the increased sensitivity to irradiation seen in banEP3622 mutants. One copy of Df(3L)Exel6086 suppresses the increased sensitivity to irradiation seen in banEP3622 mutants.
Expression of banEP3622 in wing disc clones (under the control of Scer\GAL4tub.PU) in ykiB5 mutant animals increases clone size compared to ykiB5 alone. Co-expression of dmScer\UAS.cZa in this background fails to rescue the growth defect of ykiB5 mutant clones, and growth rates are similar to when banEP3622 is expressed alone in ykiB5 mutant discs. However, despite the small size of the clones, cell and nucleolar size are increased when dmScer\UAS.cZa and banEP3622 are expressed in ykiB5 wing discs.
A significant increase in the level of cell death is observed in Rbf15aΔ;banEP3622 double mutant eye disc clones.
Coexpression of banEP3622 with hpoScer\UAS.cUa, both driven by Scer\GAL4GMR.PF, significantly suppresses the eye defects and eliminates the apoptosis caused by hpo overexpression. Coexpression of banEP3622 with hpoScer\UAS.cUa in the wing, driven by Scer\GAL4nub-AC-62, suppresses the Scer\GAL4nub-AC-62>hpoScer\UAS.cUa small wing phenotype. Coexpression of CycEScer\UAS.cRa with banEP3622, under the control of Scer\GAL4GMR.PF, enhances the extra ommatidial cell phenotype seen in Scer\GAL4GMR.PF>banEP3622 pupal retinae. This phenotype is not enhanced by thScer\UAS.cHa. Coexpression of banEP3622, CycEScer\UAS.cRa and thScer\UAS.cHa, under the control of Scer\GAL4GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells.
Expression of banEP3622, under the control of Scer\GAL4tub, in ykiΔ.w mutant clones generated in the wing disc rescues the lethality of these clones, resulting in clones that are larger than those generated in wild-type controls.
When E2fScer\UAS.cNa and DpScer\UAS.cDa are driven by Scer\GAL4ptc-559.1 cells in the wing disc overproliferate. The addition of banEP3622 enhances this phenotype. Fewer cells show pyknotic nuclei, though many cells still drop out of the epithelial layer.
EP3219EP3219 combined with banEP3622 has little or no effect on growth. The wing overgrowth phenotype caused by expression of banEP3622 under the control of Scer\GAL4Bx-MS1096 is not altered by Cdk43/Cdk43. Co-expression of banEP3622 almost completely suppresses the eye phenotype caused by expression of exScer\UAS.cBa under the control of Scer\GAL4hs.2sev.
Xenogenetic Interactions
Statement
Reference
Reduction of ban activity through banEP3622/Df(3L)banΔ1 enhances Hsap\MJDtr.Q78.GMR.T:Ivir\HA1-induced degeneration, such that flies are now born with more neuronal loss.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (17)