Open Close
General Information
Symbol
Dmel\bru1UASp.cFa
Species
D. melanogaster
Name
FlyBase ID
FBal0144449
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of the bru1 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mis-expression of aretScer\UAS.P\T.cFa in female germline stem cells (GSCs) and their immediate descendants under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in a progressive decline in GSC numbers per germarium within two weeks after eclosion.

In wild-type animals, all the primordial germ cells (PGCs) carry a single spherical fusome (spectrosome) or two dividing spherical fusomes at late third instar. However, all the Scer\GAL4nos.UTR.T:Hsim\VP16>aretScer\UAS.P\T.cFa-overexpressing gonads contain PGCs with branched fusomes indicative of precocious cell differentiation.

Ovarioles of aretScer\UAS.P\T.cFa; Scer\GAL4nos.UTR.T:Hsim\VP16 flies have a range of germ cell replication defects: In some cases no oocyte develops and endoreplication of the nurse cells fails. In other cases egg chambers have 31 nurse cells, and one oocyte with 5 ring canals instead of 4. In rare cases (<5%) these egg chambers form with a mislocalised oocyte, or with two oocytes. Fused egg chambers also occasionally form. aretScer\UAS.P\T.cFa; Scer\GAL4nos.UTR.T:Hsim\VP16 flies lay fewer eggs than wild-type. Of the eggs that are laid, approximately 5% are clearly ventralised, as assessed by absence or fusion of dorsal appendages. Eggs also occasionally form a posterior micropyle. About 15% of the resulting embryos have cuticles with a typical posterior group phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity for enokK1293 exacerbates the female germline stem cell loss elicited by Scer\GAL4nos.UTR.T:Hsim\VP16>aretScer\UAS.P\T.cFa overexpression.

aptScer\UAS.P\T.cFa or vasαTub67C.T:Avic\GFP do not suppress the germ cell replication defects seen in Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa ovarioles. In contrast oskScer\UAS.P\T.cFa or osk3'UTR.Scer\UAS.P\T can fully suppress these phenotypes, although oskfs(1)K10.3'UTR.Scer\UAS can not. grk3'UTR.Scer\UAS.P\T partially suppresses these phenotypes. Heterozygosity for grk2B6 enhances the penetrance of dorsal appendage loss or fusion phenotypes in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa females from 5% to 90%. This phenotype still shows over 90% penetrance in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa; grk2B6/+; vasαTub67C.T:Avic\GFP flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The early arrest phenotype seen in aretQB mutant ovaries is rescued by aretScer\UAS.P\T.cFa; Scer\GAL4nos.UTR.T:Hsim\VP16.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
aretScer\UAS.P\T.cFa
bru1Scer\UAS.P\T.cFa
bru1UASp.cFa
Name Synonyms
Secondary FlyBase IDs
    References (3)