Allele Dmel\aPKC^ΔN.Scer\UAS

General Information
Symbol	Dmel\aPKC^ΔN.Scer\UAS	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Betschinger	FlyBase ID	FBal0144482
Feature type	allele	Associated gene	Dmel\aPKC
Also Known As	aPKC^ΔN

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Betschinger et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	aPKC^Scer\UAS.cBa (Betschinger et al., 2003)
Nature of the lesion	Statement Reference Construct: Scer\UAS drives expression of a truncated aPKC consisting of amino acids 180-606. (Betschinger et al., 2003)
Carried in construct	P{UAS-aPKC.ΔN} (Langevin et al., 2005, Mayer et al., 2005, Betschinger et al., 2003, Lee et al., 2006, Djiane et al., 2005, Betschinger et al., 2006, Roegiers et al., 2005, Tian and Deng, 2008, Wirtz-Peitz et al., 2008, Zeng et al., 2010, Kaplan et al., 2011, Fletcher et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage, with Scer\GAL4^{neur-GAL4-A101} (Roegiers et al., 2005) neuroanatomy defective, with Scer\GAL4^wor.PA (Lee et al., 2006) planar polarity defective, with Scer\GAL4^sev.EP (Djiane et al., 2005)
Phenotype Manifest In
	adult Malpighian tubule \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, Scer\GAL80^αTub84B.PL (Zeng et al., 2010) eye photoreceptor cell, with Scer\GAL4^sev.EP (Djiane et al., 2005) larval brain & neuroblast \| supernumerary, with Scer\GAL4^wor.PA (Lee et al., 2006) larval brain \| posterior & neuroblast \| supernumerary (Betschinger et al., 2006) ommatidium, with Scer\GAL4^sev.EP (Djiane et al., 2005) tormogen cell \| somatic clone \| supernumerary, with Scer\GAL4^{neur-GAL4-A101} (Roegiers et al., 2005)
Detailed Description
	Statement Reference Expression of aPKC[ΔN.Scer\UAS] in adult Malpighian tubule clones (using the MARCM system, under the control of Scer\GAL80[αTub84B.PL] and Scer\GAL4[Scer\FRT.Act5C]) blocks renal and nephric stem cell (RNSC) proliferation and differentiation. (Zeng et al., 2010) Expression of aPKC^ΔN.Scer\UAS in the neuroblasts, driven by Scer\GAL4¹⁴⁰⁷, results in a mild overgrowth of neuroblasts in the posterior brain cortex. (Betschinger et al., 2006) Expression of aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^wor.PA results in a slight increase in the number of neuroblasts in the larval brain. (Lee et al., 2006) The ommatidia of aPKC^ΔN.Scer\UAS; Scer\GAL4^sev.EP adults are frequently misrotated, symmetrical or lacking some photoreceptors. (Djiane et al., 2005) Clones that express aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^{neur-GAL4-A101} show an increased frequency of adult external sense organs with supernumerary socket cells. (Roegiers et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.Scer\UAS is an enhancer of cell polarity defective phenotype of sgg^M11 (Kaplan et al., 2011) arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.Scer\UAS is an enhancer of planar polarity defective phenotype of sgg^M11 (Kaplan et al., 2011) Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is an enhancer of cell polarity defective phenotype of arm^F1α, sgg^M11 (Kaplan et al., 2011) Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is an enhancer of planar polarity defective phenotype of arm^F1α, sgg^M11 (Kaplan et al., 2011)
NOT Enhancer of
Statement Reference Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-enhancer of cell polarity defective phenotype of arm^F1α (Kaplan et al., 2011) Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-enhancer of planar polarity defective phenotype of arm^F1α (Kaplan et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-suppressor of cell polarity defective phenotype of arm^F1α (Kaplan et al., 2011) Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-suppressor of planar polarity defective phenotype of arm^F1α (Kaplan et al., 2011)
Phenotype Manifest In
Enhancer of
Statement Reference arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.Scer\UAS is an enhancer of denticle belt phenotype of sgg^M11 (Kaplan et al., 2011) Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is an enhancer of denticle belt phenotype of arm^F1α, sgg^M11 (Kaplan et al., 2011)
NOT Enhancer of
Statement Reference Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-enhancer of denticle belt phenotype of arm^F1α (Kaplan et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS is a non-suppressor of denticle belt phenotype of arm^F1α (Kaplan et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^da.G32/aPKC^ΔN.Scer\UAS rescues aPKC^k06403 (Kaplan et al., 2011)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	aPKC^ΔN.Scer\UAS aPKC^ΔN (Roegiers et al., 2005, Wirtz-Peitz et al., 2008)
Name Synonym	Saccharomyces cerevisiae UAS construct a of Betschinger (Betschinger et al., 2003)
Secondary FlyBase IDs

References ( 13 )
Research paper	Fletcher et al., 2012, Curr. Biol. 22(12): 1116--1122 Positive feedback and mutual antagonism combine to polarize crumbs in the Drosophila follicle cell epithelium. [FBrf0218646] Kaplan et al., 2011, PLoS ONE 6(4): e18616 Complex Interactions between GSK3 and aPKC in Drosophila Embryonic Epithelial Morphogenesis. [FBrf0213424] Zeng et al., 2010, J. Cell. Physiol. 224(3): 766--774 Tumor suppressors Sav/scrib and oncogene ras regulate stem-cell transformation in adult Drosophila malpighian tubules. [FBrf0211181] Tian and Deng, 2008, Development 135(3): 463--471 Lgl and its phosphorylation by aPKC regulate oocyte polarity formation in Drosophila. [FBrf0203052] Wirtz-Peitz et al., 2008, Cell 135(1): 161--173 Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization. [FBrf0206088] Betschinger et al., 2006, Cell 124(6): 1241--1253 Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. [FBrf0189901] Lee et al., 2006, Nature 439(7076): 594--598 lgl, pins and aPKC regulate neuroblast self-renewal versus differentiation. [FBrf0191245] Djiane et al., 2005, Cell 121(4): 621--631 The apical determinants aPKC and dPatj regulate Frizzled-dependent planar cell polarity in the Drosophila eye. [FBrf0187224] Langevin et al., 2005, Curr. Biol. 15(10): 955--962 Lethal giant larvae controls the localization of notch-signaling regulators numb, neuralized, and Sanpodo in Drosophila sensory-organ precursor cells. [FBrf0187315] Mayer et al., 2005, Curr. Biol. 15(20): 1847--1854 Quantitative analysis of protein dynamics during asymmetric cell division. [FBrf0190032] Roegiers et al., 2005, Mol. Biol. Cell 16(8): 3480--3487 Regulation of membrane localization of Sanpodo by lethal giant larvae and neuralized in asymmetrically dividing cells of Drosophila sensory organs. [FBrf0188062] Betschinger et al., 2003, Nature 422(6929): 326--330 The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl. [FBrf0157155]
Supplementary material	Wirtz-Peitz et al., 2008, Cell 135(1): Supplemental Data. [FBrf0211096]

Allele Dmel\aPKCΔN.Scer\UAS

Allele Dmel\aPKC^ΔN.Scer\UAS