UASt sequences drive expression of Spn27A.
embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4da.G32
embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4lz-gal4
Flies expressing Spn27AScer\UAS.cLa under the control of Scer\GAL4Act5C show a strongly reduced melanisation reaction at a wound site 16 hours after septic injury compared to control flies.
Expression of Spn27AScer\UAS.cLa ubiquitously under the control of Scer\GAL4da.G32, or in crystal cells under the control of Scer\GAL4lz-gal4, completely inhibits melanization of hemolymph clots.
Scer\GAL4btl.PS/Spn27AUAS.cLa is a suppressor of lethal phenotype of Scer\GAL4btl.PS, Spn77BadsRNA.WIZ.UAS
Scer\GAL4btl.PS/Spn27AUAS.cLa is a suppressor of adult tracheal system phenotype of Scer\GAL4btl.PS, Spn77BadsRNA.WIZ.UAS
Spn27AUAS.cLa/Scer\GAL4He.PZ is a suppressor of embryonic/larval hemolymph | third instar larval stage phenotype of Scer\GAL4He.PZ, grimUAS.cNa
Co-expression of Spn27AScer\UAS.cLa suppresses the lethality and tracheal melanisation caused by expression of Spn77BadsRNA.WIZ.Scer\UAS under the control of Scer\GAL4btl.PS.
Expression of Spn27AScer\UAS.cLa in larval hemocytes expressing grimScer\UAS.cNa under the control of Scer\GAL4He.PZ results in almost no melanization.
Scer\GAL4da.G32/Spn27AUAS.cLa rescues Spn27Aex32
The addition of Spn27AScer\UAS.cLa (driven by Scer\GAL4da.G32) suppresses both lethality and melanisation phenotypes seen in Spn27Aex32.