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General Information
Symbol
Dmel\Sod2dsRNA.UAS.cKa
Species
D. melanogaster
Name
FlyBase ID
FBal0144561
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Sod2IR, SOD2_dsRNA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Template consists of an inverted repeat of a 757bp fragment of Sod2 cDNA (from the 3' end of the cDNA (minus the PolyA signal) to a 5' EcoRI site). Expression is driven by UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Sod2dsRNA.Scer\UAS.cKa RNAi under the control of Scer\GAL4da.PU strongly reduces the survival rate of adult flies compared to controls.

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

Chronic hypoxia (5% O[[2]]) dramatically restores the abbreviated life span of flies expressing Sod2dsRNA.Scer\UAS.cKa under the control of Scer\GAL4da.G32.

Survival of flies expressing Sod2dsRNA.Scer\UAS.cKa under the control of Scer\GAL4da.G32 rapidly plummets from well over 90% to less than 25% within a day when the flies are transferred from permissive hypoxia to restrictive normoxia conditions. If flies expressing Sod2dsRNA.Scer\UAS.cKa under the control of Scer\GAL4da.G32 are transferred from normoxia to hypoxia, the high mortality of the flies rapidly arrests, and the survivors live as long as their cohorts maintained under permanent hypoxia.

Extracts from Sod2dsRNA.Scer\UAS.cKa; Scer\GAL4da.G32 flies have reduced mitochondrial aconitase activity, and reduced succinate dehydrogenase activity. aconitase activity can be recovered by treating the extract with a iron and a thiol reductant. Sod2dsRNA.Scer\UAS.cKa; Scer\GAL4da.G32 flies are hypersensitive to paraquat toxicity (survival is reduced to 2% of control levels with P{UAS-Sod2.dsRNA.K}24 and 17 % of control levels with P{UAS-Sod2.dsRNA.K}15 on exposure to 2mM paraquat. Pre-adult mortality in Sod2dsRNA.Scer\UAS.cKa; Scer\GAL4da.G32 animals is no different to wild-type, but rapid onset adult mortality in these flies leads to a severe reduction in average lifespan (86% reduction with P{UAS-Sod2.dsRNA.K}15; 76% reduction with P{UAS-Sod2.dsRNA.K}24.)

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Sod2dsRNA.Scer\UAS.cKa suppresses the lifespan extension phenotype seen when p38bScer\UAS.cAa is expressed in muscles under the control of Scer\GAL4Mef2.PR.

Expression of Sod2dsRNA.Scer\UAS.cKa does not suppress the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

dsRNA is expressed from the template to study the phenotypic consequences of dsRNA interference (RNAi) of the Sod2 gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Sod2dsRNA.Scer\UAS.cKa
Sod2dsRNA.UAS.cKa
Name Synonyms
Secondary FlyBase IDs
    References (8)