Allele Dmel\Sod2^{dsRNA.Scer\UAS.cKa}

General Information
Symbol	Dmel\Sod2^{dsRNA.Scer\UAS.cKa}	Species	D. melanogaster
Name		FlyBase ID	FBal0144561
Feature type	allele	Associated gene	Dmel\Sod2

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kirby et al., 2002) in vitro construct - RNAi (Kirby et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Template consists of an inverted repeat of a 757bp fragment of Sod2 cDNA (from the 3' end of the cDNA (minus the PolyA signal) to a 5' EcoRI site). Expression is driven by Scer\UAS sequences. (Kirby et al., 2002)
Carried in construct	P{UAS-Sod2.dsRNA.K} (Kirby et al., 2002, Missirlis et al., 2003, Wicks et al., 2009, Sinenko et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	short lived, with Scer\GAL4^da.G32 (Kirby et al., 2002, Wicks et al., 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Chronic hypoxia (5% O[[2]]) dramatically restores the abbreviated life span of flies expressing Sod2[dsRNA.Scer\UAS.cKa] under the control of Scer\GAL4[da.G32]. Survival of flies expressing Sod2[dsRNA.Scer\UAS.cKa] under the control of Scer\GAL4[da.G32] rapidly plummets from well over 90% to less than 25% within a day when the flies are transferred from permissive hypoxia to restrictive normoxia conditions. If flies expressing Sod2[dsRNA.Scer\UAS.cKa] under the control of Scer\GAL4[da.G32] are transferred from normoxia to hypoxia, the high mortality of the flies rapidly arrests, and the survivors live as long as their cohorts maintained under permanent hypoxia. (Wicks et al., 2009) Extracts from Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 flies have reduced mitochondrial aconitase activity, and reduced succinate dehydrogenase activity. aconitase activity can be recovered by treating the extract with a iron and a thiol reductant. Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 flies are hypersensitive to paraquat toxicity (survival is reduced to 2% of control levels with P{UAS-Sod2.dsRNA.K}24 and 17 % of control levels with P{UAS-Sod2.dsRNA.K}15 on exposure to 2mM paraquat. Pre-adult mortality in Sod2^{dsRNA.Scer\UAS.cKa}; Scer\GAL4^da.G32 animals is no different to wild-type, but rapid onset adult mortality in these flies leads to a massive reduction in average lifespan (86% reduction with P{UAS-Sod2.dsRNA.K}15; 76% reduction with P{UAS-Sod2.dsRNA.K}24.) (Kirby et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Sod2^{dsRNA.Scer\UAS.cKa}/Scer\GAL4^Mef2.PR is a suppressor of long lived phenotype of Scer\GAL4^Mef2.PR, p38b^Scer\UAS.cAa (Vrailas-Mortimer et al., 2011)
NOT Suppressor of
Statement Reference Sod2^{dsRNA.Scer\UAS.cKa}, Scer\GAL4^Hml.Δ is a non-suppressor of increased cell number \| third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa, Scer\GAL4^Hml.Δ (Sinenko et al., 2010)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Sod2^{dsRNA.Scer\UAS.cKa}, Scer\GAL4^Hml.Δ is a non-suppressor of embryonic/larval hemocyte \| third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa, Scer\GAL4^Hml.Δ (Sinenko et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of Sod2[dsRNA.Scer\UAS.cKa] suppresses the lifespan extension phenotype seen when p38b[Scer\UAS.cAa] is expressed in muscles under the control of Scer\GAL4[Mef2.PR]. (Vrailas-Mortimer et al., 2011) Expression of Sod2[dsRNA.Scer\UAS.cKa] does not suppress the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1[Scer\UAS.cSa] is expressed under the control of Scer\GAL4[Hml.Δ]. (Sinenko et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	24489 w¹; P{UAS-Sod2.dsRNA.K}15/SM5
Notes on Origin
Discoverer

Comments
	dsRNA is expressed from the template to study the phenotypic consequences of dsRNA interference (RNAi) of the Sod2 gene. (Kirby et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Sod2^{dsRNA.Scer\UAS.cKa}
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Vrailas-Mortimer et al., 2011, Dev. Cell 21(4): 783--795 A Muscle-Specific p38 MAPK/Mef2/MnSOD Pathway Regulates Stress, Motor Function, and Life Span in Drosophila. [FBrf0216446] Sinenko et al., 2010, Blood 116(22): 4612--4620 Genetic manipulation of AML1-ETO-induced expansion of hematopoietic precursors in a Drosophila model. [FBrf0212361] Wicks et al., 2009, Free Radical Biol. Med. 46(2): 176--181 Hypoxia rescues early mortality conferred by superoxide dismutase deficiency. [FBrf0206850] Missirlis et al., 2003, J. Biol. Chem. 278(48): 47365--47369 Compartment-specific protection of iron-sulfur proteins by superoxide dismutase. [FBrf0167750] Kirby et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99(25): 16162--16167 RNA interference-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress. [FBrf0156041]

Allele Dmel\Sod2dsRNA.Scer\UAS.cKa

Allele Dmel\Sod2^{dsRNA.Scer\UAS.cKa}