FlyBase Allele Report: Scer\GAL4[Orco.2.642.Hsim\VP22]

General Information

Symbol

Scer\GAL4^{Orco.2.642.Hsim\VP22}

Species

S. cerevisiae

Name

FlyBase ID

FBal0144592

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Orco-GAL4.W}

Also Known As

Orco-Gal4, Or83b-Gal4, Or83bGAL4, pOrco-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Wang et al., 2003)

Progenitor genotype

Carried in construct

P{Orco-GAL4.W}

Cytology

Description

2.642kb of Orco promoter sequences drive expression of GAL4 fused to Hsim\VP22. The addition of VP22 does not affect the activity of the GAL4 protein produced.

(Wang et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

Orco

Orco_Or83bCRM

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval olfactory receptor neuron | restricted

(Masuda-Nakagawa et al., 2009)

adult stage

olfactory receptor neuron of basiconic sensillum of antennal segment 3 | subset

(Tanoue et al., 2004)

olfactory receptor neuron of maxillary palp basiconic sensillum | subset

(Tanoue et al., 2004)

maxillary palp basiconic sensillum | subset

(Tanoue et al., 2004)

olfactory receptor neuron | subset

(Root et al., 2008, Suh et al., 2004, Tanoue et al., 2004, Wang et al., 2003)

basiconic sensillum of antennal segment 3 | subset

(Tanoue et al., 2004)

adult olfactory receptor neuron | subset

(Shang et al., 2007, Suh et al., 2004)

adult olfactory receptor neuron Or22

(Ronderos et al., 2014, Shang et al., 2007)

adult olfactory receptor neuron Or47a

(Shang et al., 2007)

adult olfactory receptor neuron Or92a

(Shang et al., 2007)

adult olfactory receptor neuron

(Kain et al., 2008, Lai and Lee, 2006)

antenna | restricted

(Kain et al., 2008, Root et al., 2008)

third segment of antenna | restricted

(Kain et al., 2008)

maxillary palp | restricted

(Root et al., 2008)

adult antennal lobe | restricted

(Root et al., 2008)

adult antennal lobe glomerulus | subset

(Wang et al., 2003)

adult antennal lobe glomerulus

(Lai and Lee, 2006)

Additional Information

Statement

Reference

Drives expression in about 80% of adult olfactory receptor neurons, and in antennal glomeruli with the exception of VM1 and DP1m.

(FlyBase Curators, 2017)

ScerGAL4^{Orco.2.642.HsimVP22} drives expession in adult olfactory receptor neuron Or83c (ai2a neuron).

(Ronderos et al., 2014)

Expression is observed in about 70% of olfactory receptor neurons.

(Tanoue et al., 2004)

Expression is observed in about 80% of olfactory neurons, but not in GR21A⁺ neurons.

(Suh et al., 2004)

ScerGAL4^{Or83b.2.642.T:HsimVP22} is estimated to express in approximately 80% of all olfactory receptor neurons.

(Suh et al., 2004)

Adult expression is observed in 80% of olfactory neurons, and in antennal glomeruli with the exception of VM1 and DP1m.

(Wang et al., 2003)

Marker for

adult antennal lobe projection neuron

(Wang et al., 2003)

adult olfactory receptor neuron

(Suh et al., 2004)

Reflects expression of

Orco

(Masuda-Nakagawa et al., 2009, Kain et al., 2008, Root et al., 2008, Shang et al., 2007, Lai and Lee, 2006, Suh et al., 2004, Tanoue et al., 2004, Wang et al., 2003)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior, with Dll^JF02499

(Plavicki et al., 2012)

abnormal chemosensory behavior, with Or42b^UAS.cGMa

(Gomez-Marin and Louis, 2014)

abnormal chemosensory behavior | adult stage, with Ctet\tetX^TNT-LC.UAS

(Badel et al., 2016, Kwon et al., 2010)

abnormal chemosensory behavior | adult stage, with Gαq^JF01209

(Kwon et al., 2010)

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Kwon et al., 2010)

abnormal chemosensory behavior | adult stage, with norpA^GD10490

(Kwon et al., 2010)

abnormal chemosensory behavior | adult stage, with slo^GD244

(Kwon et al., 2010)

abnormal chemosensory behavior | adult stage | heat sensitive, with shi^1.UAS

(Gao et al., 2015)

abnormal chemosensory behavior | larval stage, with Ctet\tetX^TNT-LC.UAS

(Tastekin et al., 2015)

abnormal chemosensory behavior | third instar larval stage, with ChT^KK109385

(Hamid et al., 2021)

abnormal circadian rhythm, with Clk^{Δ.UAS.Tag:HA}

(Tanoue et al., 2004)

abnormal circadian rhythm, with cyc^{Δ.UAS.Tag:MYC}

(Tanoue et al., 2004)

abnormal courtship behavior, with fru^RNAi.M.UAS

(Manoli et al., 2005)

abnormal courtship behavior, with GABA-B-R2^RNAi.UAS

(Root et al., 2008)

abnormal feeding behavior, with Clk^DN.UAS

(Xu et al., 2008)

abnormal learning | adult stage, with Ctet\tetX^TNT-LC.UAS

(Eddison et al., 2012)

abnormal learning | adult stage, with for^UAS.T1

(Eddison et al., 2012)

abnormal locomotor behavior | adult stage | :heat sensitive | chemical conditional, with shi^1.UAS

(Gao et al., 2015)

abnormal locomotor behavior | larval stage, with Ctet\tetX^TNT-LC.UAS

(Tastekin et al., 2015)

abnormal neuroanatomy, with Cbeta\DT^A-I.UAS

(Sweeney et al., 2011)

abnormal neurophysiology, with GABA-B-R2^RNAi.UAS

(Root et al., 2008)

abnormal neurophysiology, with Gprk2^UAS.cTa

(Tanoue et al., 2008)

abnormal neurophysiology, with Gαq^RNAi.UAS.1f1

(Kain et al., 2008)

abnormal neurophysiology, with Plc21C^GD11359

(Kain et al., 2008)

abnormal neurophysiology, with rdgA^GD1518

(Kain et al., 2008)

abnormal neurophysiology, with TkR99D^RNAi.UAS

(Ignell et al., 2009)

abnormal neurophysiology | adult stage, with Orco², Orco^S289A.UAS

(Poudel et al., 2021)

abnormal neurophysiology | adult stage, with Pkc98E^FRT, Scer\FLP1^UAS.cUa

(Poudel et al., 2021)

abnormal neurophysiology | adult stage, with Pkc98E^RNAi.UAS.cUa

(Poudel et al., 2021)

abnormal neurophysiology | drug conditional, with Bper\ptxA^tetO.cCa, Tn10\tetR^{rtTA(M2-alt).UAS}

(Chatterjee et al., 2009)

abnormal neurophysiology | heat sensitive, with Bper\ptxA^act.UAS, Scer\GAL80^ts.αTub84B

(Chatterjee et al., 2009)

abnormal phototaxis, with Crei\ChR2^UAS.cSa

(Bellmann et al., 2010)

abnormal phototaxis, with Egra\PACα^UAS.cSLa

(Bellmann et al., 2010)

abnormal smell perception, with Cbeta\DT^A-I.UAS

(Fishilevich et al., 2005)

abnormal smell perception, with Or42a^UAS.cFa

(Gomez-Marin et al., 2011)

abnormal smell perception, with TkR99D^RNAi.UAS

(Ignell et al., 2009)

abnormal smell perception, with TkR99D^UAS.EGFP

(Ignell et al., 2009)

abnormal smell perception | adult stage | conditional, with Cam^KK109037

(Bahk and Jones, 2016)

abnormal smell perception | drug conditional, with Bper\ptxA^tetO.cCa, Tn10\tetR^{rtTA(M2-alt).UAS}

(Chatterjee et al., 2009)

abnormal smell perception | heat sensitive, with Bper\ptxA^act.UAS, Scer\GAL80^ts.αTub84B

(Chatterjee et al., 2009)

increased cell death, with Cbeta\DT^A-I.UAS

(Fishilevich et al., 2005)

Phenotype Manifest In

adult antennal lobe projection neuron VA1d adPN, with Cbeta\DT^A-I.UAS

(Sweeney et al., 2011)

adult olfactory receptor neuron Or22 | conditional, with Cam^KK109037

(Bahk and Jones, 2016)

adult olfactory receptor neuron Or59b, with Plc21C^GD11359

(Kain et al., 2008)

adult olfactory receptor neuron Or59b | conditional, with Cam^KK109037

(Bahk and Jones, 2016)

adult olfactory receptor neuron Or67d, with Orco², Orco^S289A.UAS

(Poudel et al., 2021)

adult olfactory receptor neuron Or67d, with Pkc98E^FRT, Scer\FLP1^UAS.cUa

(Poudel et al., 2021)

adult olfactory receptor neuron Or67d, with Pkc98E^RNAi.UAS.cUa

(Poudel et al., 2021)

antenna, with Gprk2^UAS.cTa

(Tanoue et al., 2008)

antennal basiconic sensillum ab1 | heat sensitive, with Bper\ptxA^act.UAS, Scer\GAL80^ts.αTub84B

(Chatterjee et al., 2009)

embryonic/larval olfactory receptor neuron, with Cbeta\DT^A-I.UAS

(Sweeney et al., 2011)

synaptic vesicle, with TkR99D^RNAi.UAS

(Ignell et al., 2009)

Detailed Description

Statement

Reference

Inactivation of a subset of Scer\GAL4^{Orco.2.642.Hsim\VP22} cells (using Ctet\tetX^{TNT-LC.FRT.stop.UAS} and Scer\FLP1³³⁵) in males has little effect on the frequency of successful matings, copulation duration or copulation latency, compared to controls.

(Jois et al., 2018)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^{Orco.2.642.Hsim\VP22} cells in flying adults results in small changes to odor stream avoidance and attraction, compared to controls.

(Badel et al., 2016)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^{Orco.2.642.Hsim\VP22} cells in adult females significantly reduces attraction to vinegar, with no effect on avoidance of E2-hexenal or acetic acid, compared to controls.

(Gao et al., 2015)

Ablation of Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Inhibition (using Ctet\TeTxLC^TNT.UAS) of Scer\GAL4^{Orco.2.642.Hsim\VP22} neurons in larvae results in an increase in the average distance maintained between themselves and an appetitive odor, compared to controls.

(Tastekin et al., 2015)

Inhibition (using Ctet\TeTxLC^TNT.UAS) of Scer\GAL4^{Orco.2.642.Hsim\VP22} cells does not affect the preference of larvae for larval-treated substrate, compared to controls.

(Mast et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Crei\ChR2^UAS.cSa, Scer\GAL4^{Orco.2.642.Hsim\VP22} has abnormal phototaxis phenotype, suppressible by norpA^P24

(Bellmann et al., 2010)

Egra\PACα^UAS.cSLa, Scer\GAL4^{Orco.2.642.Hsim\VP22} has abnormal phototaxis phenotype, suppressible by norpA^P24