FlyBase Allele Report: Dmel\SNF4Aγ[loe]

General Information

Symbol

Dmel\SNF4Aγ^loe

Species

D. melanogaster

Name

FlyBase ID

FBal0144604

Feature type

allele

Associated gene

Dmel\SNF4Aγ

Associated Insertion(s)

P{lacW}SNF4Aγ^loe

Carried in Construct

Also Known As

loe

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Tschäpe et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}SNF4Aγ^loe

Cytology

Description

P{lacW} insertion is within exon 7 of the loeII transcript.

(Tschäpe et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Tschäpe et al., 2002)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is ameliorated by Hmgcr^clb2

(Tschäpe et al., 2002)

is exacerbated by Hmgcr^UAS.cvDa

(Tschäpe et al., 2002)

is ameliorated by Hmgcr^clb1

(Tschäpe et al., 2002)

is exacerbated by Appl^d

(Tschäpe et al., 2002)

is ameliorated by Rho1^72F

(Cook et al., 2012)

is exacerbated by Rho1^V14.UAS

(Cook et al., 2012)

is ameliorated by Fpps^k03514

(Cook et al., 2012)

is ameliorated by Fpps^k06103

(Cook et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Cook et al., 2012)

abnormal neuroanatomy | adult stage

(Wentzell et al., 2012)

abnormal neuroanatomy | third instar larval stage

(Wentzell et al., 2012)

abnormal phototaxis

(Cook et al., 2012)

Phenotype Manifest In

adult brain

(Wentzell et al., 2012, Tschäpe et al., 2002)

adult central complex

(Tschäpe et al., 2002)

adult central nervous system

(Tschäpe et al., 2002)

eye

(Cook et al., 2012)

larval brain | third instar larval stage

(Wentzell et al., 2012)

vacuole

(Cook et al., 2012)

Detailed Description

Statement

Reference

SNF4Aγ^loe homo- and heterozygous mutants exhibit age-dependent degeneration of the nervous system.

SNF4Aγ^loe exhibit a mild rough eye phenotype.

(Cook et al., 2012)

SNF4Aγ^loe mutant third instar larval brains show increased vacuolisation compared to controls. The phenotype is more severe in males than in females. An increased number of vacuoles is also seen in 4 day old adult flies.

(Wentzell et al., 2012)

Mutants exhibit severe vacuolisation of the central nervous system. The vacuolar pathology is most prominent around the central complex and the central parts of the brain. Neurons appear to undergo necrotic cell death. Mutants also have ~40% reduced levels of cholesterol ester.

(Tschäpe et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

SNF4Aγ^loe has abnormal neuroanatomy phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^Appl.G1a

(Cook et al., 2012)

SNF4Aγ^loe has abnormal phototaxis phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^Appl.G1a

(Cook et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | adult stage phenotype, enhanceable by Appl^d

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Scer\GAL4^Appl.G1a/Appl^sd.UAS

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Scer\GAL4^Appl.G1a/Bace^UAS.cCSa

(Wentzell et al., 2012)

NOT Enhanced by

Statement

Reference

SNF4Aγ^loe has abnormal neuroanatomy phenotype, non-enhanceable by Rab5^k08230

(Cook et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy phenotype, non-enhanceable by Cdc42³

(Cook et al., 2012)

Suppressed by

Statement

Reference

SNF4Aγ^loe has abnormal neuroanatomy phenotype, suppressible by Fpps[+]/Fpps^k06103

(Cook et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy phenotype, suppressible by Rho1^72F/Rho1[+]

(Cook et al., 2012)

SNF4Aγ^loe has abnormal phototaxis phenotype, suppressible by Rho1^72F/Rho1[+]

(Cook et al., 2012)

Appl^d, SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Appl.G1a/Appl^UAS.cCSa

(Wentzell et al., 2012)

Hsap\APP^{s.β.UAS.Tag:HA}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\BACE1^UAS.cGa

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^Appl.G1a/Appl^UAS.cCSa

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by kuz^UAS.cFa/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\APP^695.UAS.Exel/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\APP^{s.α.UAS.Tag:HA}/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^Appl.G1a/Hsap\APP^{s.β.UAS.Tag:HA}

(Wentzell et al., 2012)

NOT suppressed by

Statement

Reference

SNF4Aγ^loe has abnormal neuroanatomy phenotype, non-suppressible by Rab5^k08230

(Cook et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy phenotype, non-suppressible by Cdc42³

(Cook et al., 2012)

Appl^d, SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Appl^{dAICD.UAS.Tag:HA}/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

Other

Statement

Reference

Rho1^72F/Rho1[+], SNF4Aγ^loe has long lived phenotype

(Cook et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

SNF4Aγ^loe has vacuole phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^Appl.G1a

(Cook et al., 2012)

SNF4Aγ^loe has adult brain phenotype, enhanceable by Appl^d

(Wentzell et al., 2012, Tschäpe et al., 2002)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, enhanceable by Scer\GAL4^Appl.G1a/Appl^sd.UAS

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, enhanceable by Scer\GAL4^Appl.G1a/Bace^UAS.cCSa

(Wentzell et al., 2012)

SNF4Aγ^loe has adult brain phenotype, enhanceable by Scer\GAL4^Appl.G1a/Hmgcr^UAS.cvDa

(Tschäpe et al., 2002)

Suppressed by

Statement

Reference

SNF4Aγ^loe has vacuole phenotype, suppressible by Fpps[+]/Fpps^k03514

(Cook et al., 2012)

SNF4Aγ^loe has vacuole phenotype, suppressible by Rho1^72F/Rho1[+]

(Cook et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^Appl.G1a/Appl^UAS.cCSa

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by kuz^UAS.cFa/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by Hsap\APP^695.UAS.Exel/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by Hsap\APP^{s.α.UAS.Tag:HA}/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^Appl.G1a/Hsap\APP^{s.β.UAS.Tag:HA}

(Wentzell et al., 2012)

Appl^d, SNF4Aγ^loe has larval brain | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Appl.G1a/Appl^UAS.cCSa

(Wentzell et al., 2012)

Hsap\APP^{s.β.UAS.Tag:HA}, SNF4Aγ^loe, Scer\GAL4^Appl.G1a has larval brain | third instar larval stage phenotype, suppressible by Hsap\BACE1^UAS.cGa

(Wentzell et al., 2012)

SNF4Aγ^loe has adult brain phenotype, suppressible by Hmgcr^clb2

(Tschäpe et al., 2002)

SNF4Aγ^loe has adult brain phenotype, suppressible by Hmgcr^clb1

(Tschäpe et al., 2002)

NOT suppressed by

Statement

Reference

Appl^d, SNF4Aγ^loe has larval brain | third instar larval stage phenotype, non-suppressible by Appl^s.UAS.Tag:HA/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

SNF4Aγ^loe has larval brain | third instar larval stage phenotype, non-suppressible by Appl^{dAICD.UAS.Tag:HA}/Scer\GAL4^Appl.G1a

(Wentzell et al., 2012)

NOT Enhancer of

Statement

Reference

SNF4Aγ^loe is a non-enhancer of eye phenotype of Rho1^V14.UAS, Scer\GAL4^GMR.PF

(Cook et al., 2012)

SNF4Aγ^loe is a non-enhancer of retina phenotype of Rho1^V14.UAS, Scer\GAL4^GMR.PF

(Cook et al., 2012)

SNF4Agamma[+]/SNF4Aγ^loe is a non-enhancer of eye phenotype of cathD¹

(Kuronen et al., 2009)

NOT Suppressor of

Statement

Reference

SNF4Aγ^loe is a non-suppressor of retina phenotype of Rho1^V14.UAS, Scer\GAL4^GMR.PF

(Cook et al., 2012)

SNF4Aγ^loe is a non-suppressor of eye phenotype of Rho1^V14.UAS, Scer\GAL4^GMR.PF

(Cook et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

A Fpps^k06103 heterozygous background significantly suppresses the degenerative nervous sytem phenotype found in 5 day old SNF4Aγ^loe mutants. These flies exhibit a reduction in the area of vacuoles in the optic system, from approximately 163 υm[2] to 33υm[2] in a Fpps^k03514/+ background and 25υm[2] in a Fpps^k06103/+ background. There is a similar reduction in the number of vacuoles present, confirming an involvement of the isoprenoid pathway in the degenerative phenotype of SNF4Aγ^loe flies.

There is no significant difference in the nervous system degeneration seen in SNF4Aγ^loe mutants in a Cdc42³ or Rab5^k08230 heterozygous background.

A heterozygous Rho1^72F background suppresses the neuronal vacuolization seen in SNF4Aγ^loe mutants to approximately half the level in single mutants. The number of vacuoles is also reduced, compared to SNF4Aγ^loe single mutants.

Rho1^72F heterozygosity increases the performance index of SNF4Aγ^loe flies in a fast phototaxis assay.

Expression of constitutively-active Rho1^V14.Scer\UAS pan-neuronally, under the control of Scer\GAL4^Appl.G1a, enhances the neural degeneration and vacuolization seen in SNF4Aγ^loe mutants.

Expression of Rho1^V14.Scer\UAS under the control of Scer\GAL4^Appl.G1a decreases the performance index of SNF4Aγ^loe flies in a fast phototaxis assay.

A SNF4Aγ^loe mutant background does not affect the external or internal eye phenotype caused by overexpression of Rho1^V14.Scer\UAS under the control of Scer\GAL4^GMR.PF.

Rho1^72F/+; SNF4Aγ^loe/SNF4Aγ^loe flies show a significant increase in lifespan compared to SNF4Aγ^loe mutants.

(Cook et al., 2012)

Expression of Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Appl^{dAICD.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a is unable to suppress the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Appl^sd.Scer\UAS under the control of Scer\GAL4^Appl.G1a significantly enhances the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a enhances the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of kuz^Scer\UAS.cFa under the control of Scer\GAL4^Appl.G1a suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Hemizygosity for Appl^d enhances the vacuolisation seen in 4 day old SNF4Aγ^loe mutant males.

Expression of Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a partially suppresses the vacuolisation seen in 4 day old Appl^d SNF4Aγ^loe double mutant males, returning the phenotype to the level seen in SNF4Aγ^loe single mutants.

Expression of Appl^{s.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a fails to suppress the vacuolisation seen in 4 day old Appl^d SNF4Aγ^loe double mutant males.

(Wentzell et al., 2012)

The addition of Hmgcr^clb1 or Hmgcr^clb2 to SNF4Aγ^loe animals shows a small but significant reduction in the number of holes seen in the adult brain. The addition of Hmgcr^{Scer\UAS.cvDa} (driven by Scer\GAL4^Appl.G1a) enhances the adult brain degeneration phenotype seen in SNF4Aγ^loe animals. The addition of Appl^d enhances the adult brain degeneration phenotype seen in SNF4Aγ^loe animals.

(Tschäpe et al., 2002)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\APP^{695.Scer\UAS.Exel} under the control of Scer\GAL4^Appl.G1a dramatically suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Hsap\APP^{s.α.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Hsap\APP^{s.β.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Co-expression of Hsap\BACE1^Scer\UAS.cGa suppresses the increase in vacuolisation seen when Hsap\APP^{695.Scer\UAS.Exel} is expressed in the brains of SNF4Aγ^loe mutant third instar larvae under the control of Scer\GAL4^Appl.G1a.

(Wentzell et al., 2012)

Complementation and Rescue Data

Rescued by

SNF4Aγ^loe is rescued by SNF4Aγ^loeI.UAS/Scer\GAL4^elav.PLu

(Tschäpe et al., 2002)

Partially rescued by

SNF4Aγ^loe is partially rescued by SNF4Aγ^{Δ1-319.loeI.UAS}/Scer\GAL4^elav.PLu

(Tschäpe et al., 2002)

SNF4Aγ^loe is partially rescued by SNF4Aγ^{Δ1-738.loeI.UAS}/Scer\GAL4^elav.PLu

(Tschäpe et al., 2002)

Not rescued by

SNF4Aγ^loe is not rescued by SNF4Aγ^loeII.UAS/Scer\GAL4^elav.PLu