A Fppsk06103 heterozygous background significantly suppresses the degenerative nervous sytem phenotype found in 5 day old SNF4Aγloe mutants. These flies exhibit a reduction in the area of vacuoles in the optic system, from approximately 163 υm to 33υm in a Fppsk03514/+ background and 25υm in a Fppsk06103/+ background. There is a similar reduction in the number of vacuoles present, confirming an involvement of the isoprenoid pathway in the degenerative phenotype of SNF4Aγloe flies.
A heterozygous Rho172F background suppresses the neuronal vacuolization seen in SNF4Aγloe mutants to approximately half the level in single mutants. The number of vacuoles is also reduced, compared to SNF4Aγloe single mutants.
Expression of ApplScer\UAS.cCSa under the control of Scer\GAL4Appl.G1a partially suppresses the vacuolisation seen in 4 day old Appld SNF4Aγloe double mutant males, returning the phenotype to the level seen in SNF4Aγloe single mutants.
The addition of Hmgcrclb1 or Hmgcrclb2 to SNF4Aγloe animals shows a small but significant reduction in the number of holes seen in the adult brain. The addition of HmgcrScer\UAS.cvDa (driven by Scer\GAL4Appl.G1a) enhances the adult brain degeneration phenotype seen in SNF4Aγloe animals. The addition of Appld enhances the adult brain degeneration phenotype seen in SNF4Aγloe animals.
Co-expression of Hsap\BACE1Scer\UAS.cGa suppresses the increase in vacuolisation seen when Hsap\APP695.Scer\UAS.Exel is expressed in the brains of SNF4Aγloe mutant third instar larvae under the control of Scer\GAL4Appl.G1a.