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General Information
Symbol
Dmel\SNF4Aγloe
Species
D. melanogaster
Name
FlyBase ID
FBal0144604
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
loe
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
P{lacW} insertion is within exon 7 of the loeII transcript.
Insertion components
P{lacW}SNF4Aγloe
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is ameliorated by Hmgcrclb2
is ameliorated by Hmgcrclb1
is exacerbated by Appld
is ameliorated by Rho172F
is exacerbated by Rho1V14.UAS
is ameliorated by Fppsk03514
is ameliorated by Fppsk06103
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
SNF4Aγloe homo- and heterozygous mutants exhibit age-dependent degeneration of the nervous system. SNF4Aγloe exhibit a mild rough eye phenotype.
SNF4Aγloe mutant third instar larval brains show increased vacuolisation compared to controls. The phenotype is more severe in males than in females. An increased number of vacuoles is also seen in 4 day old adult flies.
Mutants exhibit severe vacuolisation of the central nervous system. The vacuolar pathology is most prominent around the central complex and the central parts of the brain. Neurons appear to undergo necrotic cell death. Mutants also have ~40% reduced levels of cholesterol ester.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
SNF4Aγloe has neuroanatomy defective phenotype, non-enhanceable by Cdc423
Suppressed by
Statement
Reference
SNF4Aγloe has neuroanatomy defective phenotype, suppressible by Rho172F/Rho1[+]
SNF4Aγloe has phototaxis defective phenotype, suppressible by Rho172F/Rho1[+]
SNF4Aγloe has neuroanatomy defective phenotype, suppressible by Fpps[+]/Fppsk06103
NOT suppressed by
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
SNF4Aγloe has vacuole phenotype, suppressible by Fpps[+]/Fppsk03514
SNF4Aγloe has vacuole phenotype, suppressible by Rho172F/Rho1[+]
SNF4Aγloe has adult brain phenotype, suppressible by Hmgcrclb2
SNF4Aγloe has adult brain phenotype, suppressible by Hmgcrclb1
NOT suppressed by
NOT Enhancer of
Statement
Reference
SNF4Aγloe is a non-enhancer of eye phenotype of Rho1V14.UAS, Scer\GAL4GMR.PF
SNF4Aγloe is a non-enhancer of retina phenotype of Rho1V14.UAS, Scer\GAL4GMR.PF
SNF4Agamma[+]/SNF4Aγloe is a non-enhancer of eye phenotype of cathD1
NOT Suppressor of
Statement
Reference
SNF4Aγloe is a non-suppressor of retina phenotype of Rho1V14.UAS, Scer\GAL4GMR.PF
SNF4Aγloe is a non-suppressor of eye phenotype of Rho1V14.UAS, Scer\GAL4GMR.PF
Additional Comments
Genetic Interactions
Statement
Reference
A Fppsk06103 heterozygous background significantly suppresses the degenerative nervous sytem phenotype found in 5 day old SNF4Aγloe mutants. These flies exhibit a reduction in the area of vacuoles in the optic system, from approximately 163 υm[2] to 33υm[2] in a Fppsk03514/+ background and 25υm[2] in a Fppsk06103/+ background. There is a similar reduction in the number of vacuoles present, confirming an involvement of the isoprenoid pathway in the degenerative phenotype of SNF4Aγloe flies. There is no significant difference in the nervous system degeneration seen in SNF4Aγloe mutants in a Cdc423 or Rab5k08230 heterozygous background. A heterozygous Rho172F background suppresses the neuronal vacuolization seen in SNF4Aγloe mutants to approximately half the level in single mutants. The number of vacuoles is also reduced, compared to SNF4Aγloe single mutants. Rho172F heterozygosity increases the performance index of SNF4Aγloe flies in a fast phototaxis assay. Expression of constitutively-active Rho1V14.Scer\UAS pan-neuronally, under the control of Scer\GAL4Appl.G1a, enhances the neural degeneration and vacuolization seen in SNF4Aγloe mutants. Expression of Rho1V14.Scer\UAS under the control of Scer\GAL4Appl.G1a decreases the performance index of SNF4Aγloe flies in a fast phototaxis assay. A SNF4Aγloe mutant background does not affect the external or internal eye phenotype caused by overexpression of Rho1V14.Scer\UAS under the control of Scer\GAL4GMR.PF. Rho172F/+; SNF4Aγloe/SNF4Aγloe flies show a significant increase in lifespan compared to SNF4Aγloe mutants.
Expression of ApplScer\UAS.cCSa under the control of Scer\GAL4Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of Appls.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of AppldAICD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a is unable to suppress the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of Applsd.Scer\UAS under the control of Scer\GAL4Appl.G1a significantly enhances the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of BaceScer\UAS.cCSa under the control of Scer\GAL4Appl.G1a enhances the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of kuzScer\UAS.cFa under the control of Scer\GAL4Appl.G1a suppresses the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Hemizygosity for Appld enhances the vacuolisation seen in 4 day old SNF4Aγloe mutant males. Expression of ApplScer\UAS.cCSa under the control of Scer\GAL4Appl.G1a partially suppresses the vacuolisation seen in 4 day old Appld SNF4Aγloe double mutant males, returning the phenotype to the level seen in SNF4Aγloe single mutants. Expression of Appls.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a fails to suppress the vacuolisation seen in 4 day old Appld SNF4Aγloe double mutant males.
The addition of Hmgcrclb1 or Hmgcrclb2 to SNF4Aγloe animals shows a small but significant reduction in the number of holes seen in the adult brain. The addition of HmgcrScer\UAS.cvDa (driven by Scer\GAL4Appl.G1a) enhances the adult brain degeneration phenotype seen in SNF4Aγloe animals. The addition of Appld enhances the adult brain degeneration phenotype seen in SNF4Aγloe animals.
Xenogenetic Interactions
Statement
Reference
Expression of Hsap\APP695.Scer\UAS.Exel under the control of Scer\GAL4Appl.G1a dramatically suppresses the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of Hsap\APPs.α.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Expression of Hsap\APPs.β.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγloe mutant third instar larvae. Co-expression of Hsap\BACE1Scer\UAS.cGa suppresses the increase in vacuolisation seen when Hsap\APP695.Scer\UAS.Exel is expressed in the brains of SNF4Aγloe mutant third instar larvae under the control of Scer\GAL4Appl.G1a.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)