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General Information
Symbol
Dmel\Rfx253
Species
D. melanogaster
Name
FlyBase ID
FBal0144622
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C10365235T

Reported nucleotide change:

C1899T

Amino acid change:

S449F | Rfx-PC; S420F | Rfx-PF; S398F | Rfx-PG; S457F | Rfx-PH; S427F | Rfx-PI; S435F | Rfx-PJ

Reported amino acid change:

S435F

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C1899T. Amino acid replacement: S435F. Nucleotide coordinates relative to AJ133103.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

chordotonal organ & leg sensillum (with Df(3R)S143702)

chordotonal organ & leg sensillum & scolopidial cilium (with Df(3R)S143702)

Johnston's organ & scolopale (with Rfx49)

Johnston's organ & scolopidial cilium (with Rfx49)

Johnston's organ & scolopidium (with Rfx49)

sensillum campaniformium & wing sensillum (with Rfx49)

sensillum campaniformium & wing sensillum & dendrite (with Rfx49)

Detailed Description
Statement
Reference

Homozygotes die as larvae under standard rearing conditions but under uncrowded conditions, some survive to the pupal stage. Rfx49/Rfx253 larvae show no sensitivity to either an attractive (butanol) or repulsive (n-octyl-acetate) odour, in contrast to wild type. Rfx49/Rfx253 larvae do not avoid high concentrations of NaCl, in contrast to wild type. Rfx49/Rfx253 larvae show normal phototaxis behaviour. Rfx49/Rfx253 and Rfx253/Df(3R)S143702 adult escapers have a characteristic uncoordinated phenotype; the flies cannot stand or walk, legs are crossed and wings are held up. The flies react to light stimulation by moving their legs and wings in an effort to right themselves, but they are limited to a few steps or flip from one side to another. The average resting transepithelial potential (TEP) of macrochaetae (recordings have been performed on either the posterior dorsocentral or anterior scutellar bristle) in Rfx49/Rfx253 adults is significantly lower than in control siblings. No mechanically elicited changes in TEP are detected in the mutant bristles. No receptor currents are elicited by mechanical stimulation in the mutant bristles. The resistance at mutant bristles is on average, one-third of that at control bristles. No sound-elicited potentials are detected in the antennal nerve of Rfx49/Rfx253 adults, in contrast to wild type. Rfx49/Rfx253 adults show defects in the wing campaniform sensilla; the dendrite is never situated under the dome-shaped sensillum (as occurs in wild type) and sometimes ends well before the sensillum. In most cases, the cilium is shorter than normal or absent. Rfx253/Df(3R)S143702 adults show defects in the chordotonal organ of the femur; the cilium is either absent or shorter than normal and distinct subregions of the cilium cannot be distinguished, in contrast to wild type. The dendritic transition zone appears swollen. Rfx49/Rfx253 adults show no gross defects in Johnston's organ, but the ultrastructure of individual scolopidia is profoundly altered; no typical cilia are seen and the anchoring axial filament appears to be missing. The scolopale rods that enclose the cilium are not as regularly arrayed as in wild-type flies. The dendritic cap appears normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Complements: jumu06142a. Complements: jumu06439. Complements: stich1EP359. Complements: stich1D233. Complements: l(3)A143.1F3A143.1F3.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)