Allele Mmus\Ppp3ca^Act.Scer\UAS

General Information
Symbol	Mmus\Ppp3ca^Act.Scer\UAS	Species	M. musculus
Name		FlyBase ID	FBal0144735
Feature type	allele	Associated gene	Mmus\Ppp3ca

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Gajewski et al., 2003) in vitro construct - regulatory fusion (Gajewski et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of amino acids 1-398 of Mmus\Ppp3ca is under the control of Scer\UAS sequences. This truncation deletes the autoinhibitory and calmodulin-binding domains, making the phosphatase activity constitutive. (Gajewski et al., 2003)
Carried in construct	P{UAS-Mmus\Ppp3ca.act} (Gajewski et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^how-24B (Gajewski et al., 2003)
Phenotype Manifest In
	eye \| P-stage, with Scer\GAL4^how-24B (Gajewski et al., 2003) pupal cuticle, with Scer\GAL4^how-24B (Gajewski et al., 2003) pupal epidermis, with Scer\GAL4^how-24B (Gajewski et al., 2003) pupal thorax, with Scer\GAL4^how-24B (Gajewski et al., 2003)
Detailed Description
	Statement Reference At the beginning of pupariation about 25% of Mmus\Ppp3ca^Act.Scer\UAS; Mmus\Ppp3ca^Act.Scer\UAS animals fail to completely evert their anterior spiracles and retain an elongated and sometimes curved larval shape, with abdominal contraction away from the pupal case. Most other animals initiate prepupal or pupal development, with the latter everting their head sacs and evaginating wing and leg discs during day 1. However, these animals often show a cleft thorax phenotype because of an incomplete fusion of the epidermis. Externally, these animals fail to progress in developmental terms by day 4 or later as no eye, cuticle, or bristle pigmentation is observed. (Gajewski et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Mmus\Ppp3ca^Act.Scer\UAS, Scer\GAL4^how-24B/Scer\GAL4^how-24B has lethal \| pupal stage phenotype, suppressible by CanB2^EP774/CanB2[+], Scer\GAL4^how-24B/Scer\GAL4^how-24B (Gajewski et al., 2003)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference CanB2^EP774/+ can recue the pupal lethality of Mmus\Ppp3ca^Act.Scer\UAS; Mmus\Ppp3ca^Act.Scer\UAS animals, resulting in the emergence of adults that are able to fly. (Gajewski et al., 2003)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Mmus\Ppp3ca^Act.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Gajewski et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(3): 1040--1045 Requirement of the calcineurin subunit gene canB2 for indirect flight muscle formation in Drosophila. [FBrf0156033]

Allele Mmus\Ppp3caAct.Scer\UAS

Allele Mmus\Ppp3ca^Act.Scer\UAS