Lrev6-3 mutant eye clones exhibit a domain specific phenotype. Loss-of-function Lrev6-3 clones in the ventral eye result in the selective loss of eye fate. However, in the dorsal eye these clones have no effect on the eye fate.
Lrev6-3 clones in they eye disc show TUNEL-positive cells in the ventral eye (indicating cell death), while cells in the dorsal half of the eye are not affected.
Heterozygotes have normal eyes. Homozygous clones in the eye result in elimination of the ventral eye, but have no effect in the dorsal eye.
Homozygous clones in the dorsal part of the eye do not affect ommatidial development. Homozygous clones in the ventral part of the eye inhibit ommatidial development. Homozygous clones generated during the first larval instar can completely eliminate eye fate in the eye-antennal disc, whereas antennal development in the same disc is unaffected.
Homozygous embryos fail to complete germ band retraction and show no cuticle formation. Homozygous clones in the ventral part of eye disc, induced during the first larval instar, result in a large reduction of the ventral part of the eye disc in the third larval instar, with the surrounding heterozygous tissue and wild-type clonal tissue generally disappearing together with the homozygous clonal tissue. This results in adult eyes in which most of the ventral region of the eye is replaced by the cuticle. Homozygous clones of considerable size in the dorsal part of the eye disc, induced during the first larval instar, do not cause any obvious reduction in size of the dorsal part of the eye disc, and photoreceptor differentiation and polarity appears unaffected by these clones. Homozygous clones induced in ventral or dorsal regions of the eye disc during the late second and the third larval instars do not result in any obvious eye defects.