Allele Dmel\E2f2^dsRNA.cSa

General Information
Symbol	Dmel\E2f2^dsRNA.cSa	Species	D. melanogaster
Name		FlyBase ID	FBal0144988
Feature type	allele	Associated gene	Dmel\E2f2

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Stevaux et al., 2002, Frolov et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Template corresponds to E2f2 sequences. (Stevaux et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference SL2 cells treated with E2f2^dsRNA.cSa dsRNA have a similar cell cycle distribution profile to control treated cells. (Dimova et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference E2f2^dsRNA.cSa is a suppressor \| partially of mitotic cell cycle defective phenotype of E2f^dsRNA.cFa (Frolov et al., 2003)
Phenotype Manifest In
Suppressor of
Statement Reference E2f2^dsRNA.cSa is a suppressor \| partially of mitotic cell cycle phenotype of E2f^dsRNA.cFa (Frolov et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The addition of E2f2^dsRNA.cSa dsRNA to SL2 cells treated with E2f^dsRNA.cFa dsRNA allows the E2f2-deficient cells to enter S phase, but does not rescue the cell cycle phasing defects. Although some SL2 cells treated with both E2f^dsRNA.cFa and E2f2^dsRNA.cSa dsRNAs can enter S phase, these cells fail to proliferate. No increase in apoptosis is evident, suggesting that this failure to proliferate is not due to cell death. The proportion of G2-phase cells remains low, indicating that few of the BrdU-positive cells fully complete S phase. SL2 cells treated with E2f^dsRNA.cFa, E2f2^dsRNA.cSa and dap^dsRNA.cFa dsRNAs show a similar cell cycle distribution as wild-type cells and these cell cultures increase in number, although at a rate that is approximately half that of control cells. (Frolov et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	dsRNA transcribed from the template was transfected into S2 cells to study the phenotypic consequences of dsRNA interference (RNAi) of the E2f2 gene. (Moon et al., 2005) dsRNA has been synthesised and used to treat SL2 cells, to study the effects of dsRNA interference (RNAi) of the E2f2 gene. (Frolov et al., 2003, Dimova et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	E2f2^dsRNA.cSa
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Moon et al., 2005, Dev. Cell 9(4): 463--475 Drosophila E2F1 has context-specific pro- and antiapoptotic properties during development. [FBrf0190216] Dimova et al., 2003, Genes Dev. 17(18): 2308--2320 Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway. [FBrf0162136] Frolov et al., 2003, Genes Dev. 17(6): 723--728 G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression. [FBrf0158944] Stevaux et al., 2002, EMBO J. 21(18): 4927--4937 Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2. [FBrf0151952]

Allele Dmel\E2f2dsRNA.cSa

Allele Dmel\E2f2^dsRNA.cSa