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General Information
Symbol
Dmel\DCTN2-p50hs.PD
Species
D. melanogaster
Name
heat shock construct of Duncan
FlyBase ID
FBal0145072
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of full length DCTN2-p50 ORF (from cDNA LD07994) is under the control of an Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eggs from Dmnhs.PD females heat shocked for 1 hour have dorsal patterning defects of varying severity. In milder cases the dorsal appendages are positioned correctly along the anterior-posterior axis, but are fused at the base or along their entire length. In more severe cases the dorsal appendages are highly reduced or absent, and their rudiments are displaced posteriorly. The highest frequency of dorsal appendage defects occurs in eggs collected 20-25 hours post heat-shock (45%) and 25-30 hours after heat shock (33%), approximately corresponding to heat shock at stage 8-9 of oogenesis. However, the proportion showing posterior displacement of dorsal appendage rudiments never exceeds 5%. The incidence of dorsal appendage fusion is significantly increased in flies carrying 4 copies of Dmnhs.PD heat shocked for 30 minutes 5 times over a 5 hour period, but there is no increase in the numbers of eggs with posteriorly displaced dorsal appendages. 12 hours after a 1 hour heat shock, a small but significant proportion of stage 9 Dmnhs.PD oocytes (4-9%) have a severely mis-positioned nucleus. In these cases the nucleus may remain associated with the cortex, or detach completely from it: about half of misplaced nuclei are clearly separated from the cortical layer of microfilaments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Additional Comments
Genetic Interactions
Statement
Reference

The percentage of eggs with fused dorsal appendage laid by heat shocked Dmnhs.PD flies is increased by heterozygosity for Df(3L)10H or by the presence of Glt.hs. However, there is no increase in the percentage of eggs with posterior shifting of the dorsal appendage rudiments, or of stage 10 oocytes with misplaced nuclei in either case. Heterozygosity for Khc8 or Klc8ex94 dramatically increases the percentage of eggs laid by heat shocked Dmnhs.PD flies with displaces and/or highly reduced dorsal appendages.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
DCTN2-p50hs.PD
DmnHsp70.PD
Dmnhs.PD
Name Synonyms
heat shock construct of Duncan
Secondary FlyBase IDs
    References (2)