Allele Dmel\Dg^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Dg^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0145086
Feature type	allele	Associated gene	Dmel\Dg

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Deng et al., 2003) in vitro construct - RNAi (Deng et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drives expression of a Dg hairpin loop (that should produce dsRNA when expressed). (Deng et al., 2003)
Carried in construct	P{UAS-Dg.dsRNA} (Schneider et al., 2006, Deng et al., 2003, Poulton and Deng, 2006, Haines et al., 2007, Shcherbata et al., 2007, Bogdanik et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective \| adult stage \| conditional, with Scer\GAL4^how-24B (Shcherbata et al., 2007) locomotor behavior defective \| adult stage \| conditional, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^GMR.PU (Shcherbata et al., 2007) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^repo.PU (Shcherbata et al., 2007) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) neurophysiology defective \| larval stage, with Scer\GAL4^how-24B (Bogdanik et al., 2008) partially lethal - majority die \| larval stage, with Scer\GAL4^69B (Haines et al., 2007) partially lethal - majority die \| larval stage, with Scer\GAL4^tub.PU (Haines et al., 2007) viable, with Scer\GAL4^how-24B (Haines et al., 2007)
Phenotype Manifest In
	embryonic/larval muscle system \| third instar larval stage, with Scer\GAL4^how-24B (Haines et al., 2007) embryonic/larval muscle system \| third instar larval stage, with Scer\GAL4^tub.PU (Haines et al., 2007) embryonic/larval neuromuscular junction, with Scer\GAL4^how-24B (Bogdanik et al., 2008) eye disc \| third instar larval stage, with Scer\GAL4^GMR.PU (Shcherbata et al., 2007) eye disc \| third instar larval stage, with Scer\GAL4^repo.PU (Shcherbata et al., 2007) eye disc \| third instar larval stage, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) eye photoreceptor cell, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) eye photoreceptor cell \| third instar larval stage, with Scer\GAL4^GMR.PU (Shcherbata et al., 2007) eye photoreceptor cell \| third instar larval stage, with Scer\GAL4^repo.PU (Shcherbata et al., 2007) eye photoreceptor cell \| third instar larval stage, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) follicle cell, with Scer\GAL4^αTub84B.PL (Deng et al., 2003) indirect flight muscle \| conditional, with Scer\GAL4^how-24B (Shcherbata et al., 2007) indirect flight muscle \| conditional, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) lamina plexus \| third instar larval stage, with Scer\GAL4^GMR.PU (Shcherbata et al., 2007) lamina plexus \| third instar larval stage, with Scer\GAL4^repo.PU (Shcherbata et al., 2007) lamina plexus \| third instar larval stage, with Scer\GAL4^tub.PU (Shcherbata et al., 2007) sarcomere \| third instar larval stage, with Scer\GAL4^how-24B (Haines et al., 2007) sarcomere \| third instar larval stage, with Scer\GAL4^tub.PU (Haines et al., 2007)
Detailed Description
	Statement Reference The mean amplitude of the evoked junctional current (EJC) is decreased by approximately 40% at the neuromuscular junction in larvae expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B] compared to in controls. The miniature junctional current (mEJC) amplitude is not decreased in the mutant animals. Quantal content is reduced by approximately 40% in the mutant larvae. Expression of Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[elav.PU] does not significantly affect the mean amplitude of the mEJC or the quantal content at the larval neuromuscular junction. (Bogdanik et al., 2008) Third instar larval muscle sarcomeres are significantly smaller in animals expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[tub] compared to controls. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment. Third instar larval muscle sarcomeres are much more variable in size in animals expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B] compared to controls at 25[o]C. Individual larvae show either small or larger sarcomeres in each muscle, and a combination of both small and large sarcomeres in a single muscle is not seen. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment. Third instar larval muscle sarcomeres are larger in animals expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B] compared to controls at 30[o]C. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment. There is no effect on third instar larval muscle size or muscle sarcomere size in animals expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[69B] compared to controls. Expression of Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B], Scer\GAL4[tub], or Scer\GAL4[69B] does not affect crawling behaviour of third instar larvae. There is significantly higher muscle membrane resistance in larvae expressing Dg[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B] at either 25[o]C or 30[o]C compared to controls. (Haines et al., 2007) Flies expressing Dg[dsRNA.Scer\UAS] under the control of either Scer\GAL4[tub] or the muscle-specific driver Scer\GAL4[how-24B], show comparable climbing ability at the beginning of adult life to control flies. However, the ability of these animals to climb declines significantly faster over time compared to controls. By 12 days (when expression is driven by Scer\GAL4[tub]) or 20 days (when expression is driven by Scer\GAL4[how-24B]), age-dependent muscle degeneration is observed including loss of muscle fibre organisation, vacuolisation and the absence of some muscles. Retinal photoreceptor cells are not elongated in adult eyes expressing Dg[dsRNA.Scer\UAS] under the control of either Scer\GAL4[tub]. Expression of Dg[dsRNA.Scer\UAS] in the eye disc (under the control of Scer\GAL4[GMR.PU]), or in all glial cells (under the control of Scer\GAL4[repo.PU]), both result in photoreceptor axon targeting defects. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. Expression of Dg[dsRNA.Scer\UAS] in the mesodermal tissue (under the control of Scer\GAL4[how-24B]) does not effect the axon termination process. (Shcherbata et al., 2007) Expression of Dg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in a multi-layered follicle epithelium. (Deng et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Dg^{dsRNA.Scer\UAS} is a suppressor \| cell autonomous \| partially \| somatic clone of cell polarity defective \| somatic clone \| cell autonomous phenotype of Ras85D^ΔC40B (Poulton and Deng, 2006) Scer\GAL4^Act.PU/Dg^{dsRNA.Scer\UAS} is a suppressor \| cell non-autonomous \| partially \| somatic clone of cell polarity defective \| somatic clone \| cell non-autonomous phenotype of Ras85D^ΔC40B (Poulton and Deng, 2006)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Dg^{dsRNA.Scer\UAS} is a suppressor \| cell autonomous \| partially \| somatic clone of oocyte associated follicle cell \| somatic clone \| cell autonomous phenotype of Ras85D^ΔC40B (Poulton and Deng, 2006) Scer\GAL4^Act.PU/Dg^{dsRNA.Scer\UAS} is a suppressor \| cell non-autonomous \| partially \| somatic clone of oocyte \| somatic clone \| cell non-autonomous phenotype of Ras85D^ΔC40B (Poulton and Deng, 2006)
Additional Comments
Genetic Interactions
Statement Reference The oocyte polarity defects seen when Ras85D^ΔC40B mutant clones are made in the posterior follicle cell layer are partially rescued by expression of Dg^{dsRNA.Scer\UAS} in the clone using Scer\GAL4^Act and the 'MARCM' technique. The polarity defects of posterior follicle cells (PFCs) seen in Ras85D^ΔC40B mutant PFC clones are partially rescued by expression of Dg^{dsRNA.Scer\UAS} in the clone using Scer\GAL4^Act and the 'MARCM' technique. (Poulton and Deng, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Dg^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Bogdanik et al., 2008, PLoS ONE 3(4): e2084 Muscle dystroglycan organizes the postsynapse and regulates presynaptic neurotransmitter release at the Drosophila neuromuscular junction. [FBrf0210237] Haines et al., 2007, Mol. Biol. Cell 18(12): 4721--4730 Dystroglycan and protein O-mannosyltransferases 1 and 2 are required to maintain integrity of Drosophila larval muscles. [FBrf0204214] Shcherbata et al., 2007, EMBO J. 26(2): 481--493 Dissecting muscle and neuronal disorders in a Drosophila model of muscular dystrophy. [FBrf0192541] Poulton and Deng, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(34): 12775--12780 Dystroglycan down-regulation links EGF receptor signaling and anterior-posterior polarity formation in the Drosophila oocyte. [FBrf0193906] Schneider et al., 2006, Development 133(19): 3805--3815 Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization. [FBrf0192630] Deng et al., 2003, Development 130(1): 173--184 Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. [FBrf0151949]

Allele Dmel\DgdsRNA.Scer\UAS

Allele Dmel\Dg^{dsRNA.Scer\UAS}