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General Information
Symbol
Dmel\Dg323
Species
D. melanogaster
Name
FlyBase ID
FBal0145090
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

3155bp deletion (nucleotides 32,345 to 35,669 of DS03910) resulting from the imprecise excision of P{EP}DgEP2241. A C to T change is present near the proximal breakpoint.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of genomic sequences, which affects the first exon of Dg and also affects mRpL34 (removing more than half of the coding sequence).

Imprecise excision of the P{EP} element, resulting in a 3155bp deletion (nucleotides 32,345 to 35,669 of DS03910) that removes the putative transcription start site and 5' UTR of Dg. A C to T change is present near the proximal breakpoint.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

No obvious defects in muscle morphology are seen in Dg323/+ mutant flies.

Induction of Dg323 homozygous somatic clones in the eye discs results in irregular ommatidial structure in adult flies.

Approximately 20% of DgO86/Dg323 flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, approximately 60% have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5 and the remainder if the flies have a complete crossvein as in wild type.

83% of eggs laid by Dg323/DgO86 females mated to wild-type males hatch.

Dg323 germline clones are arrested prior to stage 3-4 and have oocyte polarity defects.

Late second instar Dg323 larvae have one or more muscles frequently missing or misattached. Occasional additional muscle tissue is present.

Sarcomere size is larger and more variable in Dg323 larval muscles compared to controls.

Dg323 and Dg248/Dg323 mutant third instar larvae crawl normally.

Late second instar Dg248/Dg323 larvae have one or more muscles frequently missing or misattached. Occasional additional muscle tissue is present.

Sarcomere size is larger and more variable in Dg248/Dg323 larval muscles compared to controls.

Dg323 mutant ovaries show oocyte polarity defects.

Retinal photoreceptor cells are not elongated in Dg323 mutant adult eye clones. In third instar larval eye disc clones, neuronal projections from photoreceptor neurons to the optic lobes are disturbed. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. The gaps in the lamina plexus are occupied by mis-localised glial cells. When only some of the axons or glial cells are mutant for Dg323 both autonomous and non-autonomous defects in axon and glial cell positioning are observed. These axon targeting phenotypes are not observed in Dg323/+ mutant eye discs.

Actin fails to be enriched at the posterior of the oocyte in egg chambers containing homozygous germline clones and the "spreading" of the ring canals normally seen in stage 1-2 oocytes is not seen. The actin arrays in homozygous follicle cell clones are not oriented perpendicular to the anteroposterior axis, in contrast to wild type. The basal actin fibres in follicle cells adjacent to the mutant clone are also misoriented.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Dg Dys double heterozygous flies (Dg323/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Rack1EY00128 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

chifBG02820a Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

Nrkk14301 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

FhosA055 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

CG7845EMS-Mod4 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

dyscc03838 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

vimark16722 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

vimar09 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

Fkbp1400734 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

SP2353MB00605 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

mblE27 Dg323 double heterozygous flies exhibit indirect flight muscle degeneration.

Rack1EE Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

captE636 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

captE593 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

chifEY05746 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Lis-1k11702 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Lis-1k13209 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

FhosEY09842 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChR╬▒6EY13897 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChR╬▒6KG05852 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Camn339 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Rack11.8 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

uif2B7 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

uif1 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

gcmKG01117 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

gcmrA87 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Dmnk16109 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

POSHk15815 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

delKG10262 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

del3 Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

grhIM Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Dg323/+;rtP/+ third instar larvae show muscle attachment defects and large sarcomeres.

Double homozygous Dg323;rt2 larvae show changes in sarcomere size and muscle attachment defects, identical to those seen Dg323 homozygous animals.

tw1/Y;Dg323/Dg323 larvae show changes in sarcomere size and muscle attachment defects, identical to those seen Dg323 homozygous animals.

Dg323/+; Dys8-2/+ or Dg323/+; Df(3R)Dl-X43/+ double heterozygous oocytes show significant polarity defects indistinguishable from Dg323/Dg323 or Dys8-2/Df(3R)Dl-X43 mutants.

Dg323/+; Dys8-2/+ double heterozygous mutant third instar larval eye discs show disrupted neuronal projections from photoreceptor neurons to the brain optic lobes, showing breaks in the lamina surface.

Dg323/+; Df(3R)Dl-X43/+ double heterozygous mutant third instar larval eye discs show disrupted neuronal projections from photoreceptor neurons to the brain optic lobes. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly.

Dg323/dock13421, Dg323/dock04723, Dg323/+; InR34/+ and Dg323/+; InRex52.1/+ double heterozygous mutant third instar larval eye discs all show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

Dg323/chico1 double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The oocyte polarity and developmental arrest phenotypes shown by Dg323 germline clones are partially rescued by Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of DgFL.Scer\UAS.P\T.

The oocyte polarity and developmental arrest phenotypes shown by Dg323 germline clones are partially rescued by Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of DgPPSG.Scer\UAS.P\T.

The oocyte polarity and developmental arrest phenotypes shown by Dg323 germline clones are not rescued by Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of Dg2WW.Scer\UAS.P\T.

The oocyte polarity and developmental arrest phenotypes shown by Dg323 germline clones are not rescued by Scer\GAL4nos.UTR.T:Hsim\VP16-mediated expression of DgC1.Scer\UAS.P\T.

Expression of either DgFL.Scer\UAS.P\T, DgDC2.Scer\UAS.P\T or Dg4P.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 partially rescues the oocyte polarity and egg chamber growth defects of Dg323 mutants.

Expression of DgAATA.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 partially rescues the oocyte polarity defects of Dg323 mutants.

Expression of either DgPA.Scer\UAS.P\T, DgC1.Scer\UAS.P\T or DgC2.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the oocyte polarity and egg chamber growth defects of Dg323 mutants.

Expression of DgALLP.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the oocyte polarity defects of Dg323 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

One copy of P{mRpL34+t1.3} completely rescues the lethality and sterility of Df(2R)Dg323/Df(2R)Dg248 transheterozygotes. Thus the lethality of the Df(2R)Dg323 chromosome is not due to an effect on Dg, but is due to disruption of mRpL34.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (12)