FlyBase Allele Report: Dmel\Clk[ar]

General Information

Symbol

Dmel\Clk^ar

Species

D. melanogaster

Name

FlyBase ID

FBal0145123

Feature type

allele

Associated gene

Dmel\Clk

Associated Insertion(s)

Carried in Construct

Also Known As

ClkAR

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Allada et al., 2003, Stanewsky, 2003)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. The mutation destroys the GT dinucleotide required for efficient splicing at the exon 2/intron 2 splice site.

(Allada et al., 2003)

Missense mutation destroying a splicing site.

(Stanewsky, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:7,770,022..7,770,022 [-]

Nucleotide change:

G7770022A

Reported nucleotide change:

G?A

Comment:

G to A mutation in the GT splice donor sequence

(Allada et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm

(Stanewsky, 2003)

abnormal circadian rhythm | adult stage

(Lee et al., 2016)

abnormal locomotor rhythm

(Kadener et al., 2009, Kadener et al., 2008)

abnormal locomotor rhythm (with Clk^Jrk)

(Allada et al., 2003)

abnormal locomotor rhythm (with Df(3L)HnD-1)

(Allada et al., 2003)

abnormal locomotor rhythm | recessive

(Allada et al., 2003)

Phenotype Manifest In

adult heart

(Gill et al., 2015)

Detailed Description

Statement

Reference

Clk^ar homozygous adults flies either entrained to 12h:12h light:dark cycles and then moved to constant darkness or entrained to 12h:12h 24[o] C:29 [o]C temperature cycles and then moved to constant 24[o]C show arrhythmicity under both entrainment condition; temperature entrained individuals fail to show anticipatory activity to temperature transitions.

(Lee et al., 2016)

The heart period in 5-week old Clk^ar mutant flies subjected to ad libitum feeding (ARF) is similar to controls. The heart period increases on 12-hour daytime-restricted feeding (TRF). The significant reduction in arrhythmia on TRF seen in wild type is not observed in Clk^ar mutant flies.

(Gill et al., 2015)

Homozygous flies are arrhythmic for locomotor activity.

(Kadener et al., 2009)

Heterozygous animals exhibit a wild-type circadian locomotor rhythm. Homozygotes are arrhythmic. Clk^ar/Df(3L)HnD-1 and Clk^Jrk/Clk^ar animals are also arrhythmic. Under light:dark conditions homozygotes manifest a highly abnormal activity pattern: no lights-on startle response but a robust lights-off startle response. No measurable rhythmic activity is seen in constant darkness (DD), even during the first two days of DD.

(Allada et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Clk^ar has abnormal locomotor rhythm phenotype, suppressible by cyc^VP16.UAS/Scer\GAL4^tim.PE

(Kadener et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of cyc^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^tim.PE fails to rescue circadian locomotor activity rhythms in Clk^ar flies under constant darkness conditions, but in light-dark conditions most of the abnormal features of locomotor activity seen in Clk^ar flies are rescued (higher diurnal rather than night activity as well as the lights-on startle response is restored).

(Kadener et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Clk^ar is partially rescued by Clk^Tag:V5

(Kadener et al., 2009)

Clk^ar is partially rescued by Clk^Δ1-3.Tag:V5

(Kadener et al., 2009)

Clk^ar is partially rescued by Clk^Δ1-4.Tag:V5

(Kadener et al., 2009)

Clk^ar is partially rescued by Clk^UAS.Tag:HA/Scer\GAL4^cry.PE

(Allada et al., 2003)

Not rescued by

Clk^ar is not rescued by Clk^UAS.Tag:HA/Scer\GAL4^P2.4.Pdf

(Allada et al., 2003)

Comments

Clk^T:SV5\V5 significantly rescues the arrhythmic locomotor behaviour of Clk^ar flies.

Clk^{Δ1-3.T:SV5\V5} poorly rescues the arrhythmic locomotor behaviour of Clk^ar flies.

Clk^{Δ1-4.T:SV5\V5} poorly rescues the arrhythmic locomotor behaviour of Clk^ar flies.

(Kadener et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24513

y¹ w^*; Clk^ar

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

1(7)

(Allada et al., 2003)

Clk^AR

(Lerner et al., 2015, Hermann-Luibl et al., 2014)

Clk^ar

(Elya et al., 2023, Lee et al., 2016, Blanchard et al., 2010)

ClkAR

(Kadener et al., 2008)

clk^AR

(Lerner et al., 2015, Kadener et al., 2009)

clk^ar

(Gill et al., 2015)

clkAR

(Vodala et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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