FlyBase Allele Report: Dmel\Akap200[k07118a]

General Information

Symbol

Dmel\Akap200^k07118a

Species

D. melanogaster

Name

FlyBase ID

FBal0145228

Feature type

allele

Associated gene

Dmel\Akap200

Associated Insertion(s)

P{lacW}Akap200^k07118a

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Jackson and Berg, 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}Akap200^k07118a

Cytology

Description

P{lacW} insertion in the first intron.

(Jackson and Berg, 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:8,416,613..8,416,613 [+]

(Jackson and Berg, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal

(Jackson and Berg, 2002)

partially lethal (with Df(2L)N22-14)

(Jackson and Berg, 2002)

Phenotype Manifest In

egg chamber (with Df(2L)N22-14)

(Jackson and Berg, 2002)

inner nurse cell ring canal rim (with Df(2L)N22-14)

(Jackson and Berg, 2002)

mesothoracic tergum & macrochaeta | supernumerary (with Df(2L)N22-14)

(Jackson and Berg, 2002)

nurse cell (with Df(2L)N22-14)

(Jackson and Berg, 2002)

outer nurse cell ring canal rim (with Df(2L)N22-14)

(Jackson and Berg, 2002)

Detailed Description

Statement

Reference

Homozygotes are recovered at slightly less than the expected frequency. Akap200^k07118a/Df(2L)N22-14 animals show 81% viability. 19% of Akap200^k07118a/Df(2L)N22-14 adults have at least one extra notal bristle. 11% of Akap200^k07118a/Df(2L)N22-14 egg chambers have binucleate nurse cells with ring canal remnants and 3.2% have other defects. The diameters of the inner and outer rims of the ring canals are larger than normal.

(Jackson and Berg, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Akap200^k07118a/Df(2L)N22-14 is an enhancer of nurse cell phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^{VP16.nanos.UTR}

(Jackson and Berg, 2002)

Suppressor of

Statement

Reference

Akap200^k07118a/Akap200[+] is a suppressor of outer nurse cell ring canal rim phenotype of Src64B^PI

(Jackson and Berg, 2002)

Akap200^k07118a/Akap200[+] is a suppressor of inner nurse cell ring canal rim phenotype of Src64B^PI

(Jackson and Berg, 2002)

Other

Statement

Reference

Akap200^k07118a/Df(2L)N22-14, Mmus\Prkaca^mC.UAS, Scer\GAL4^{VP16.nanos.UTR} has nurse cell phenotype

(Jackson and Berg, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The small ring canal phenotype see in Src64B^PI homozygous females is dominantly suppressed by Akap200^k07118a.

The small ring canal phenotype seen in Src64B^PI homozygous females is dominantly suppressed by Akap200^k07118a.

(Jackson and Berg, 2002)

Xenogenetic Interactions

Statement

Reference

30% of egg chambers have at least one binucleate nurse cell in Akap200^k07118a/Df(2L)N22-14 females expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Jackson and Berg, 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10656

y¹ w^67c23; P{lacW}Akap200^k07118a, l(2)k07118b^k07118b/CyO

Kyoto

111209

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Akap200^k07118a P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

Separable from: l(2)k07118b^k07118b. The majority of the lethality of the original "l(2)k07118" chromosome is not due to the P{lacW}Akap200^k07118a insertion, but is due to an unrelated background mutation (l(2)k07118b^k07118b).

(Jackson and Berg, 2002)

Comments

Precise excision of the P{lacW} element reverts the Akap200^k07118a mutant phenotypes.

(Jackson and Berg, 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Akap200^k07118

(Jackson and Berg, 2002)

Akap200^k07118a

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close

General Information
Nature of the Allele
Expression Data
Human Disease Associations
Phenotypic Data
Interactions
Complementation and Rescue Data
Images
Stocks
Notes On Origin
Comments
Crossreferences
Synonyms and Secondary IDs
References