FB2025_01 , released February 20, 2025
Allele: Dmel\Syt1UAS.Tag:CALI(4C)
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General Information
Symbol
Dmel\Syt1UAS.Tag:CALI(4C)
Species
D. melanogaster
Name
FlyBase ID
FBal0146764
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-syt.4C}
Key Links
Transgenic product class
wild_type
(Marek and Davis, 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Marek and Davis, 2002)
Mutagen
in vitro construct
(Marek and Davis, 2002)
Progenitor genotype
Carried in construct
P{UAS-syt.4C}
Cytology
Description

UAS regulatory sequences drive expression of Syt1, which is tagged at the C-terminal end with a Tag:CALI(4C) tag.

(Marek and Davis, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Tagged with
Tag:CALI(4C)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vesicles at synapses in which Syt1Scer\UAS.T:Zzzz\4C (under the control of Scer\GAL4elav.PLu) is specifically photoactivated in the endocytic phase of the vesicle cycle are significantly larger than controls. Dramatically enlarged vesicular structures that often exceed 100nm are observed throughout the vesicle pool.

      (Poskanzer et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The T:Zzzz\4C tag binds the fluorescein derivative 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH). The T:Zzzz\4C tag can be used to visualise the localisation of the sytScer\UAS.T:Zzzz\4C protein after labelling with the FlAsH ligand. The binding of the FlAsH ligand to the T:Zzzz\4C tag also allows the sytScer\UAS.T:Zzzz\4C protein to be photoinactivated in vivo using the "fluorophore-assisted light inactivation" (FALI) technique, by illumination at 488nm. The inactivation of the protein is proportional to the duration of illumination, follows first-order kinetics and is specific (it does not impair syt-independent vesicle fusion).

      (Marek and Davis, 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syt1Scer\UAS.T:Zzzz\4C
      Syt1UAS.Tag:CALI(4C)
      sytScer\UAS.T:Zzzz\4C
      Name Synonyms
      Secondary FlyBase IDs
        References (3)